Chinese Journal of Tissue Engineering Research ›› 2014, Vol. 18 ›› Issue (8): 1244-1249.doi: 10.3969/j.issn.2095-4344.2014.08.016

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Preparation and biocompatibility of gamma-aminopropyl triethoxysilane/polypyrrole/ polyester monofilament

Wang Ying1, Chen Ying2, Yue Bing-fei1, 3, Tang Jin-tian1, 2, Li Ying1, Zhang Xiao-na1   

  1. 1Department of Biopharmaceutical Sciences, Beijing University of Chinese Medicine, Beijing 100102, China; 2Institute of Medical Physics and Engineering, Department of Engineering Physics, Tsinghua University, Beijing 100084, China; 3Department of Laboratory Animal Quality Testing, National Institutes for Food and Drug Control, Beijing 100050, China
  • Received:2013-12-21 Online:2014-02-19 Published:2014-02-19
  • Contact: Yue Bing-fei, Investigator, Doctoral supervisor, Department of Biopharmaceutical Sciences, Beijing University of Chinese Medicine, Beijing 100102, China; Department of Laboratory Animal Quality Testing, National Institutes for Food and Drug Control, Beijing 100050, China Corresponding author: Tang Jin-tian, Investigator, Doctoral supervisor, Department of Biopharmaceutical Sciences, Beijing University of Chinese Medicine, Beijing 100102, China; Institute of Medical Physics and Engineering, Department of Engineering Physics, Tsinghua University, Beijing 100084, China
  • About author:Wang Ying, Studying for master’s degree, Department of Biopharmaceutical Sciences, Beijing University of Chinese Medicine, Beijing 100102, China
  • Supported by:

    China Postdoctoral Science Foundation, No. 2013M530637

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Abstract:

BACKGROUND: Now most of the researches on transplant hair are based on the patients with enough hair, but there is no apparent corrective effect for large area of alopecia. Implanted artificial hairs can solve this problem.
OBJECTIVE: To make and evaluate the γ-aminopropyl triethoxysilane/polypyrrole/polyester (KH-550/PPy/PET) composite monofilament as implanted artificial hairs, and to carry out cytotoxicity tests using NCTC clone 929 cells with composite monofilament.
METHODS: KH-550/PPy/PET composite monofilament was prepared in a series of steps, including pre-spotting, alkali treatment, silane coupling agent treatment and polypyrrole coating. The viability of NCTC clone 929 cells were detected after 1, 2, 3, 5, 7 days of co-culture with composite monofilament by using cell counting kit-8.
RESULTS AND CONCLUSION: KH-550/PPy/PET composite monofilament had a smooth surface without crack. The PPy films did not come off accidentally and had good wearability. After alkali treatment, PPy quality on the surface of monofilament was significantly heavier than before. Using silane coupling agent (KH-550) could effectively enhance biocompatibility and binding force of polyester monofilaments. After co-cultured with composite monofilaments, the viability of NCTC clone 929 cells was 100%, 80.37%, 73.26%, 81.96%, 77.50% at days 1, 2, 3, 5, 7 respectively. The level of cytotoxicity was grade 1. The results show that KH-550 can effectively enhance the binding force between PPy and PET monofilament, and the prepared KH-550/PPy/PET composite monofilament has good biocompatibility and no acute toxicity.


中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程


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Key words: biocompatible materials, transplantation, hair, scalp, alopecia

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