Biocompatibility of a hydrogel skirt scaffold in artificial cornea

doi:10.3969/j.issn.2095-4344.2013.03.011

Abstract

Abstract:

BACKGROUND: Beta-tricalcium phosphate/poly (vinyl alcohol) hydrogel (β-TCP/PVA-H) composite is a material with high water content, good softness, which is beneficial to the fibroblasts growth and collagen deposition.

OBJECTIVE: To study the biocompatibility of β-TCP/PVA-H composite as an artificial cornea skirt material.

METHODS: Delayed-type hypersensitivity: Both sides of the spinal skin of the guinea pig from beginning to end were injected with A solution (the mixture of complete Freund’s adjuvant and normal saline), B solution (extraction of β-TCP/PVA-H composite or normal saline or 2-Mercaptobenzothiazole), C solution (mixture of A and B solutions at equal volume), followed by local induction and inspire. The acute systemic toxicity test: Kunming mouse tail vein was injected with normal saline and β-TCP/PVA-H composite extraction. In vitro cytotoxicity assays: MRC-5 cells were cultured by β-TCP/PVA-H composite extraction, cell culture medium and phenol-containing cell culture medium. Intradermal reaction: The extracts of β-TCP/PVA-H composite, normal saline was injected intradermally into the rabbit spine by normal saline (or sesame oil), normal saline and sesame oil.

RESULTS AND CONCLUSION: The biocompatibility experiments of β-TCP/PVA-H composite showed that delayed-type hypersensitivity was graded 0-1, acute systemic toxicity test showed no symptoms, in vitro cytotoxicity was graded 0 or 1, and skin irritations were very slight. The experiments are agreed with the relative national standards, and β-TCP/PVA-H composite skirt as artificial cornea scaffold is a qualified medical material.
Key Words: biomaterials; material biocompatibility; beta-tricalcium phosphate; poly (vinyl alcohol); hydrogel; artificial cornea; in vitro cytotoxicity; acute systemic toxicity; intradermal reaction; delayed-type hypersensitivity; other grants-supported paper

Key words: biomaterials, material biocompatibility, beta-tricalcium phosphate, poly (vinyl alcohol), hydrogel, artificial cornea, in vitro cytotoxicity, acute systemic toxicity, intradermal reaction, delayed-type hypersensitivity, other grants-supported paper

CLC Number:

R318

Qiu Gang-feng, Shi Chun-zhi, Zhou Jin-sheng, Mai Wei-xin, Ouyang Jun-jun, Wu San-qiang, Qiu Wei-yao. Biocompatibility of a hydrogel skirt scaffold in artificial cornea[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(3): 445-451.

Figures/Tables 4

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