Effects of total flavone of Rhizoma Drynariae on the coupling of angiogenesis and osteogenesis in bone remodeling through notch signaling pathway

doi:10.12307/2021.213

Abstract

Abstract: BACKGROUND: Bone defect is a clinical difficulty in orthopedics. Total flavone Rhizoma Drynariae has a definite therapeutic effect on bone defects, but the specific mechanism is not clear.
OBJECTIVE: To explore the effects of the total flavone of Rhizoma Drynariae on angiogenesis and bone formation during bone remodeling.
METHODS: Forty-eight Sprague-Dawley rats were divided into four groups, 12 in each group, including model group, traditional Chinese medicine (TCM) group, TCM + DAPT group, and DAPT group. femoral bone defect model was established in each rat and interventions with the total flavone of Rhizoma Drynariae and the Notch signaling pathway blocker DAPT were given in corresponding groups. Osteogenesis was evaluated by X-ray, Micro-CT, and hematoxylin-eosin staining at 12 weeks of interventions. The levels of vascular endothelial growth factor, CD31, Hes1, bone morphogenetic protein 2, and Notch1 in bone tissue were detected by immunohistochemistry.
RESULTS AND CONCLUSION: X-ray, Micro-CT and hematoxylin-eosin staining showed that TCM group showed significantly better osteogenesis than the other three groups. Immunohistochemistry detection indicated that compared with the model group, the average absorbance values of vascular endothelial growth factor, CD31, Hes1, bone morphogenetic protein 2, and Notch1 were significantly higher in the TCM group (P < 0.05). Compared with the DAPT group, the average absorbance values of vascular endothelial growth factor, Hes1, and bone morphogenetic protein 2 were significantly increased in the TCM+DAPT group, while there was no significant difference in the expression of CD31 and Notch1 between the two groups. These findings indicate that the total flavone of Rhizoma Drynariae can promote the coupling of angiogenesis-osteogenesis in bone remodeling by activating the Notch pathway, thereby promoting bone repair.

Key words: bone defect, total flavone of Rhizoma Drynariae, angiogenesis, osteogenesis, Notch, signaling pathway, rat

CLC Number:

Huang Minling, Lu Zhaoqi, Shen Zhen, Lin Haixiong, Feng Junjie, Huang Feng, Jiang Ziwei, Cai Qunbin. Effects of total flavone of Rhizoma Drynariae on the coupling of angiogenesis and osteogenesis in bone remodeling through notch signaling pathway[J]. Chinese Journal of Tissue Engineering Research, 2021, 25(32): 5116-5122.

Figures/Tables 9

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