Protective effect of Ginkgolide B against hypoxia/reoxygenation-induced cardiomyocyte injury and its underlying mechanisms

doi:10.3969/j.issn.2095-4344.1257

Abstract

Abstract:

BACKGROUND: Ginkgolide B is extracted form ginkgo, which has been shown to inhibit cell apoptosis and platelet aggregation and has been extensively applied in the treatment of cardiovascular and cerebrovascular diseases. However, the underlying mechanism remains unclear.
OBJECTIVE: To observe the protective effect of ginkgolide B on hypoxia/reoxygenation-induced cardiomyocyte injury and its underlying mechanisms.
METHODS: H9C2 myocardial cells were divided into control group (common medium), ginkgolide B group (medium containing ginkgolide B), control plus ERK inhibitor group (common medium, ERK inhibitor prior to hypoxia/reoxygenation) and ginkgolide B plus ERK inhibitor group (medium containing ginkgolide B, ERK inhibitor prior to hypoxia/reoxygenation). The apoptosis rate, proliferation rate and p-ERK1/2 protein expression were detected.
RESULTS AND CONCLUSION: (1) Compared with the control group, the apoptosis rate was significantly decreased, and the proliferation rate and the p-ERK1/2 protein expression were significantly increased in the ginkgolide B group (P < 0.05). (2) Compared with the ginkgolide B group, the apoptosis rate was significantly increased, and the proliferation rate and the p-ERK1/2 protein expression were significantly decreased in the ginkgolide B plus ERK inhibitor group (P < 0.05). (3) Compared with the control plus ERK inhibitor group, the apoptosis rate was significantly decreased and the proliferation rate was significantly increased in the ginkgolide B plus ERK inhibitor group (P < 0.05), and the p-ERK1/2 protein expression showed no significant difference. (4) These results indicate that ginkgolide B can reduce the cell apoptosis of hypoxia/reoxygenation-induced cardiomyocyte injury, and improve cell survival, thereby protecting the myocardium, which is by increasing p-ERK1/2 protein expression.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: anoxia, reoxygenation, H9C2 myocardial cells, apoptosis, ERK1/2

CLC Number:

Zhang Xu1, Chen Wangsheng2, Feng Jian3, Zhang Mengjiao1, Jiang Wenjie1, Liang Xuemei1. Protective effect of Ginkgolide B against hypoxia/reoxygenation-induced cardiomyocyte injury and its underlying mechanisms [J]. Chinese Journal of Tissue Engineering Research, 2019, 23(19): 3097-3101.

Figures/Tables 3

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