Abstract:

BACKGROUND: Dysfunction of glucose metabolism is the main clinical manifestation of diabetes mellitus. Exercise is one of the main methods to treat diabetes mellitus. The effect of early exercise with different loads on the modeling rate and blood glucose level of late-stage type 2 diabetic rats is unclear.
OBJECTIVE: To explore the effects of different exercise loads on the establishment of rat diabetes mellitus model with high-sugar and high-fat diet combined with streptozotocin injection.
METHODS: Fifty Sprague-Dawley male rats (provided by Hubei Provincial Laboratory Animal Research Center) were randomly divided into quiet, diabetes mellitus, and low-, moderate-, and high-intensity exercise groups (n=10/group). Rats in the quiet group were fed with normal feed, diabetes mellitus group were fed with high-sugar and high-fat diets, and were freely active in cages. Mice in the low-, moderate-, and high-intensity exercise groups were fed with high-sugar and high-fat diets, and underwent 8-week exercise of different intensities. At the end of exercise, the body weight and free blood glucose in each group were tested, and then the rats were fasted but free to drink for 12 hours overnight. Quiet group was given intraperitoneal injection of citrate buffer, and other groups were injected intraperitoneally with 2% streptozotocin solution. The body weight, free blood glucose and glycated serum protein on day 7 were detected.
RESULTS AND CONCLUSION: The body weight, free blood glucose, glycated serum protein, and area under the curve of blood glucose in each exercise group were improved compared with the diabetes mellitus group. Moderate- and high-intensity exercise can effectively regulate blood glucose level and improve glucose tolerance of type 2 diabetes mellitus. Besides, high-intensity exercise is more effective in reducing the incidence of type 2 diabetes mellitus.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: type 2 diabetes mellitus, treadmill running, high intensity exercise, body mass, free glucose, glycated serum protein, the Natural Science Foundation of Hubei Province