Chinese Journal of Tissue Engineering Research ›› 2019, Vol. 23 ›› Issue (19): 3092-3096.doi: 10.3969/j.issn.2095-4344.1256
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Jin Shanhu, Hu Yazhe
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the Natural Science Foundation of Hubei Province, No. 2015CFA037 (to HYZ)
Abstract:
BACKGROUND: Dysfunction of glucose metabolism is the main clinical manifestation of diabetes mellitus. Exercise is one of the main methods to treat diabetes mellitus. The effect of early exercise with different loads on the modeling rate and blood glucose level of late-stage type 2 diabetic rats is unclear. OBJECTIVE: To explore the effects of different exercise loads on the establishment of rat diabetes mellitus model with high-sugar and high-fat diet combined with streptozotocin injection. METHODS: Fifty Sprague-Dawley male rats (provided by Hubei Provincial Laboratory Animal Research Center) were randomly divided into quiet, diabetes mellitus, and low-, moderate-, and high-intensity exercise groups (n=10/group). Rats in the quiet group were fed with normal feed, diabetes mellitus group were fed with high-sugar and high-fat diets, and were freely active in cages. Mice in the low-, moderate-, and high-intensity exercise groups were fed with high-sugar and high-fat diets, and underwent 8-week exercise of different intensities. At the end of exercise, the body weight and free blood glucose in each group were tested, and then the rats were fasted but free to drink for 12 hours overnight. Quiet group was given intraperitoneal injection of citrate buffer, and other groups were injected intraperitoneally with 2% streptozotocin solution. The body weight, free blood glucose and glycated serum protein on day 7 were detected. RESULTS AND CONCLUSION: The body weight, free blood glucose, glycated serum protein, and area under the curve of blood glucose in each exercise group were improved compared with the diabetes mellitus group. Moderate- and high-intensity exercise can effectively regulate blood glucose level and improve glucose tolerance of type 2 diabetes mellitus. Besides, high-intensity exercise is more effective in reducing the incidence of type 2 diabetes mellitus.
中国组织工程研究杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松;组织工程
Key words: type 2 diabetes mellitus, treadmill running, high intensity exercise, body mass, free glucose, glycated serum protein, the Natural Science Foundation of Hubei Province
CLC Number:
R446.1
R318
Jin Shanhu, Hu Yazhe. Effect of early different intensities of exercise on the modelling rate and blood glucose level of rats with late-stage type 2 diabetes[J]. Chinese Journal of Tissue Engineering Research, 2019, 23(19): 3092-3096.
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2.1 实验动物数量分析 实验选用大鼠50只,分为5组,于实验结束前大强度运动干预组死亡1只,其余全部进入结果分析。 2.2 各组大鼠体质量的比较 各组初始体质量差异无显著性意义(P > 0.05);运动干预第8周末,只有糖尿病对照组体质量比安静对照组有显著性增加(P < 0.05),而大强度运动干预组体质量相对于糖尿病对照组、小强度运动干预组、中等强度运动干预组体质量有明显下降(P < 0.05);与运动干预第8周末比较,注射链脲佐菌素第7天安静对照组和中等强度运动干预组体质量没有显著性变化,而糖尿病对照组、小强度运动干预组、大强度运动干预组体质量均明显下降(P < 0.05);注射链脲佐菌素第7天,大强度运动干预组体质量比其他4组都低(P < 0.05),见表1。"
2.3 各组大鼠空腹血糖比较 初始及运动干预第8周末各组空腹血糖差异无显著性意义(P > 0.05);与运动干预第8周末相比,注射链脲佐菌素第7天造模各组空腹血糖都有明显升高(P < 0.05);注射链脲佐菌素第7天,造模各组空腹血糖比安静对照组都有明显升高(P < 0.05),而中等强度运动干预组、大强度运动干预组空腹血糖低于糖尿病对照组、小强度运动干预组(P < 0.05),见表2。"
2.4 各组大鼠糖化血清蛋白浓度的比较 与安静对照组相比,糖尿病对照组、小强度运动干预组、大强度运动干预组糖化血清蛋白浓度有明显升高(P < 0.05);与糖尿病对照组相比,中等强度运动干预组糖化血清蛋白浓度有明显下降(P < 0.05),见表3。"
2.5 各组大鼠血糖曲线下面积(AUCFBG)比较 造模各组大鼠血糖曲线下面积(AUCFBG)都大于安静对照组(P < 0.05);糖尿病对照组、小强度运动干预组之间差异无显著性意义(P > 0.05);中等强度运动干预组、大强度运动干预组显著低于糖尿病对照组、小强度运动干预组(P < 0.05);中等强度运动干预组、大强度运动干预组之间差异无显著性意义(P > 0.05),见表4,图1。"
2.6 各组大鼠成模率比较 注射链脲佐菌素第7天,大鼠尾静脉取血检测随机血糖判定各组大鼠成模率,安静对照组没有成模大鼠,糖尿病对照组、小强度运动干预组、中等强度运动干预组、大强度运动干预组成模率分别为90%,70%,50%,44%,见表5。"