Chinese Journal of Tissue Engineering Research ›› 2019, Vol. 23 ›› Issue (6): 883-887.doi: 10.3969/j.issn.2095-4344.1537

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Effect of dental ceramic alloys on the expression of apoptosis-related signal proteins in the oral buccal mucosa of golden hamsters

Chen Run1, 2, Qiu Bingyan3, Jiang Lei1, 2, Pan Yu1, 4, Wang Yinghui1, 2, Cheng Hui1, 2, 4   

  1. 1Department of Prosthodontics, Hospital of Stomatology, Fujian Medical University, Fuzhou 350002, Fujian Province, China; 3Departmetn of Stomatology, Fujian Provincial Governmental Hospital, Fuzhou 350001, Fujian Province, China; 2Institute of Stomatology, 4Center for Stomatology Aesthetics and Biomechanics, Fujian Medical University, Fuzhou 350002, Fujian Province, China
  • Received:2018-09-01 Online:2019-02-28 Published:2019-02-28
  • Contact: Cheng Hui, Professor, Chief physician, Institute of Stomatology, Center for Stomatology Aesthetics and Biomechanics, Fujian Medical University, Fuzhou 350002, Fujian Province, China
  • About author:Chen Run, Master, Associate chief physician, Department of Prosthodontics, Hospital of Stomatology, Fujian Medical University, Fuzhou 350002, Fujian Province, China; Institute of Stomatology, Fujian Medical University, Fuzhou 350002, Fujian Province, China
  • Supported by:

    the National Natural Science Foundation of China, No. 81641158 (to CH); the Natural Science Foundation of Fujian Province, No. 2016J01354 (to CH)

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Abstract:

BACKGROUND: Dental porcelain alloys hold different levels of cytotoxicity, which can lead to cell apoptosis. However, the in vitro researches on dental ceramic alloy causing apoptosis are mainly conducted, in which the oral environment cannot be simulated well.

OBJECTIVE: To investigate the effects of dental ceramic alloys on apoptotic protein expression in the oral buccal mucosa of golden hamsters.
METHODS: Thirty-six 60-70-day-old male golden hamsters provided by Beijing Vital River Laboratory Animal Technology Co., Ltd. were randomly divided into six groups. Five kinds of dental ceramic alloys (nickel-chromium, cobalt chromium, CPTi, palladium based and gold-platinum alloys) and polypropylene (negative control) were fixed on the oral mucosa of golden hamsters in corresponding groups. The mucosae contacting with the specimens were removed at 14 and 28 days, respectively. The protein expression levels of Caspase-3, Caspase-8, and Caspase-9 in the buccal mucosa were detected by western blot assay.
RESULTS AND CONCLUSION: (1) At 14 and 28 days of fixation, there was no significant difference in the Caspase-3 protein level between gold-platinum alloy group and negative control groups (P > 0.05), and the level of Caspase-3 protein in the remaining four groups was significantly higher than that in the negative control group (P < 0.05). (2) At 14 days, the Caspase-8 protein level in each group was significantly higher than that in the negative control group (P < 0.05). After 28 days of fixation, the protein level of Caspase-8 in the nickel-chromium and palladium group was higher than that in the negative control group (P < 0.05), while the protein level of Caspase-8 in the other groups was not significantly different from that in the negative control group (P > 0.05). (3) At 14 days, the Caspase-9 protein level in each group was higher than that in the negative control group (P < 0.05). At 28 days, there was no significant difference in the Caspase-9 protein level between cobalt-chromium and negative control groups (P > 0.05), while the Caspase-9 protein level in the remaining groups was higher than that in the negative control group (P < 0.05). These results indicate that the use of five dental alloys can upregulate the levels of apoptosis-related signal molecules to different degrees.  

Key words: Metal Ceramic Alloys, Apoptosis, Dipodomys, Tissue Engineering

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