Gubi Tongxiao Granule effects on the cell apoptosis in a rabbit model of steroid-induced osteonecrosis of the femeral head

doi:10.3969/j.issn.2095-4344.0303

Abstract

Abstract:

BACKGROUND: Osteonecrosis of the femeral head (ONFH) is an atrophic debility of bones in Chinese medicine, the pain of which is caused by cold-induced blood stasis. Gubi Tongxiao Granule has the effects of reinforcing the kidney to strength the bone and promoting blood circulation to remove obstruction in the channels. Its clinical efficacy is satisfactory, but the underlying mechanism is unclear.
OBJECTIVE: To explore the curative efficacy of Gubi Tongxiao Granule for steroid-induced ONFH, and its effect on cell apoptosis, thus providing references for its clinical application.
METHODS: Fifty-four healthy rabbits were randomly divided into model, treatment and control (no intervention) groups (n=18 per group). The animal model of steroid-induced ONFH was established in the modeling and treatment groups, and then administered normal saline and Gubi Tongxiao Granule via gavage, respectively, once daily, for 12 consecutive weeks. At 4, 8 and 12 weeks after treatment, six animals in each group were sacrificed, and the bilateral femoral heads were removed. The empty bone lacuna rate and apoptotic index were determined, and the histological changes were observed under light microscope.
RESULTS AND CONCLUSION: The empty bone lacuna rate in the control and treatment groups was significantly lower than that in the model group at different time points after treatment (P < 0.05). There was no obvious change in necrotic empty bone lacuna in the control group. At 4 weeks after treatment, the empty bone lacunas were obvious in the model group, and increased in numbers and even broken with time increasing. At 4 weeks after treatment, empty bone lacunas were visible and increased in numbers in the treatment group. The apoptotic index in the control and treatment groups was significantly lower than that in the model group (P < 0.05). These results indicate that Gubi Tongxiao Granule is effective for steroid-induced ONFH in rabbits, can effectively inhibit cell apoptosis and improve the pathological changes of the necrotic area.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Drugs, Chinese Herbal, Models, Animal, Femur Head Necrosis, Apoptosis, Tissue Engineering

CLC Number:

R318

Zhou Zheng-xin, Li Wen-hua, Zhu Lei, Han Shi-ding. Gubi Tongxiao Granule effects on the cell apoptosis in a rabbit model of steroid-induced osteonecrosis of the femeral head[J]. Chinese Journal of Tissue Engineering Research, 2018, 22(20): 3190-3194.

Figures/Tables 1

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