Embryotoxicity of eugenol based on a model of embryonic stem cell test

doi:10.3969/j.issn.2095-4344.2015.19.011

Abstract

Abstract:

BACKGROUND: As the pharmacological effect of eugenol constantly being discovered, its application in medical and food industry becomes wider. However, its toxicity studies have not established a complete database, especially in the improvement of safety assessment of developmental toxicity and teratogenicity.
OBJECTIVE: To establish a model of embryonic stem cell test to evaluate the embryotoxicity of eugenol.
METHODS: Mouse fibroblasts (3T3) and mouse embryonic stem cells (E14TG2a) were cultured in vitro, and MTT test was performed to detect the cytotoxicity of 3T3 cells and E14TG2a cells with positive control 5-fluorouracil, negative control penicillin G and tested compound eugenol. The concentration of the tested compounds that inhibiting 50% viability of embryonic stem cells (IC50 E14TG2a) and 3T3 fibroblasts (IC50 3T3) was calculated. The hanging-suspension-adherent culture systems were used to induce embryonic stem cells into cardiomyocytes, and the concentration of tested compounds that caused 50% inhibition of differentiation of E14TG2a cells into cardiomyocytes (ID50 E14TG2a) was calculated. The embryotoxic potential of eugenol was classified by prediction model of the embryonic stem cell test.
RESULTS AND CONCLUSION: The proliferations of E14TG2a and 3T3 cells were inhibited by eugenol, of which the IC50 3T3 and IC50 E14TG2a values were (3.613±0.192) and (1.799±0.131) mg/L. The differentiation of E14TG2a was also inhibited by eugenol, of which the ID50 E14TG2a was (3.501±0.158) mg/L. Eugenol was evaluated as a chemical compound with strong embryotoxicity by the model of embryonic stem cell test.

Key words: Eugenol, Toxicity Tests, Inhibitory Concentration 50, Embryonic Stem Cells

CLC Number:

R394.2

Li Fu-gui, Chen Jing, Cheng Wei-min, Ji Ming-fang. Embryotoxicity of eugenol based on a model of embryonic stem cell test [J]. Chinese Journal of Tissue Engineering Research, 2015, 19(19): 3017-3021.

Figures/Tables 3

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