A serum-free monolayer method for differentiation of porcine induced pluripotent stem cells into vascular endothelial cells

doi:10.3969/j.issn.2095-4344.2115

Abstract

Abstract:

BACKGROUND: Induced pluripotent stem cells are a cell source with multiple differentiation potentials. Since pig is a suitable model for human cardiovascular and metabolic diseases, induction of porcine pluripotent stem cells into endothelial cells will provide a support for creating a porcine cardiovascular disease model.

OBJECTIVE: To establish a serum-free single layer culture method to induce porcine induced pluripotent stem cells into CD31 positive endothelial cells and to identify the obtained endothelial cells.

METHODS: We used the serum-free single layer culture method to induce porcine pluripotent stem cells and human embryonic stem cells into endothelial cells under different combinations of CHIR99021, bone morphogenetic protein 4, basic fibroblast growth factor and vascular endothelial growth factor through three stages. The expressions of germ layer markers of the two kinds of stem cells during their differentiation were detected and compared. The endothelial cells obtained after the flow sorting were identified.

RESULTS AND CONCLUSION: The endothelial cells differentiated from porcine induced pluripotent stem cells by a single layer culture method showed comparable morphological and functional properties to the immortalized porcine aortic endothelial cells, capable of taking up low-density lipoprotein and forming microvascular-like structure on matrigel. We found that the differentiation efficiency of porcine pluripotent stem cells was higher than that of human embryonic stem cells using the same method, which may be associated with the different expression patterns of lineage-specific markers in the two kinds of cells during the early differentiation.

Key words: porcine, induced pluripotent stem cell, differentiation, endothelial cell, human embryonic stem cells

CLC Number:

Wei Renyue, Li Xuechun, Li Yan, Yu Yang, Zhang Yu, Liu Zhonghua. A serum-free monolayer method for differentiation of porcine induced pluripotent stem cells into vascular endothelial cells[J]. Chinese Journal of Tissue Engineering Research, 2020, 24(31): 4971-4978.

Figures/Tables 5

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