Chinese Journal of Tissue Engineering Research ›› 2019, Vol. 23 ›› Issue (6): 917-923.doi: 10.3969/j.issn.2095-4344.1547

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Silver nanoparticle-doped tricalcium phosphate: in vitro and in vivo toxicity in rabbits

Cheng Jian1, 2, Zhang Jun1, 3, Guan Jie1, Zeng Junkai1, Zhao Xin1, Xie Youzhuan1   

  1. 1Department of Orthopedics, Ninth People’s Hospital of Shanghai Jiaotong University Medical School, Shangahi 200011, China; 2Fifth Department of Surgery, Yuyao People’s Hospital, Yuyao 315400, Zhejiang Province, China; 3Department of Orthopedics, Qingpu Central Hospital of Shanghai, Shanghai 200011, China
  • Received:2018-10-27 Online:2019-02-28 Published:2019-02-28
  • Contact: Xie Youzhuan, Chief physician, Department of Orthopedics, Ninth People’s Hospital of Shanghai Jiaotong University Medical School, Shangahi 200011, China
  • About author:Cheng Jian, Master candidate, Associate chief physician, Department of Orthopedics, Ninth People’s Hospital of Shanghai Jiaotong University Medical School, Shangahi 200011, China; Fifth Department of Surgery, Yuyao People’s Hospital, Yuyao 315400, Zhejiang Province, China
  • Supported by:

    the National Natural Science Foundation of China, No. 81071453 (to XYZ); the Foundation of Shanghai Science and Technology Committee, No. 1441901700 (to XYZ)

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Abstract:

BACKGROUND: Previous studies by the research team found that 5% silver nanoparticle-doped β-tricalcium phosphate material has antibacterial properties and good biocompatibility.

OBJECTIVE: To study the biotoxicity of porous β-tricalcium phosphate containing 10% mass fraction of silver nanoparticles (10% SNPs-TCP) in vitro and in vivo.
METHODS: (1) In vitro cytotoxicity: Bone marrow mesenchymal stem cells from Sprague-Dawley rats (provided by Shanghai Xipuer-Beikai Animal Co., Ltd., China) were cultured with the extract containing 10% SNPs-TCP, porous TCP extract and normal tissue culture medium for 1, 3, 5, 7 days. Cell counting kit-8 method was used to detect cell proliferation. (2) In vivo toxicity: Twenty New Zealand white rabbits (provided by Shanghai Jiagan Biotechnology Co., Ltd., China) were used to make bone defects of the left femoral condyle (6 mm in diameter and 13 mm in depth), which were randomly divided into two groups. The experimental group was implanted with 10% SNPs-TCP, and the control group was implanted with single TCP. Twelve weeks after implantation, inductively coupled plasma mass spectrometry was used to detect the concentration of silver ions in the blood and tissues. Hematoxylin-eosin staining sections were made to observe the pathological changes of various tissues and organs. Van Gieson staining sections were used to observe the tissue growth in the implantation area.

RESULTS AND CONCLUSION: (1) There was no difference in cell proliferation between the TCP group, normal culture group and 10% SNPs-TCP group at different time points (P < 0.05). (2) The concentration of silver ions in the blood, heart, liver, spleen, lung and kidney of the experimental and control groups was less than 0.1×10-6, which was less than the highest concentration of silver ions (0.5×10-6)in the human body specified by the WHO. (3) There were no obvious pathological changes in the heart, liver, spleen, lung and kidney sections of the experimental and control groups. (4) The contours of the implantation materials in the experimental and control groups could be distinguished. New bone tissues stained with red were around the materials. A large amount of red-stained new bone tissues were visible inside the porous structure of the materials. There was no significant difference in the distribution of new bone tissue in the porous structure of the two groups of materials. To conclude, the 10% SNPs-TCP has no obvious cytotoxicity and toxicity in animals, and has good biocompatibility.

Key words: Silver, Calcium Phosphates, Toxicity Tests, Tissue Engineering

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