Effects of different growth factors on biological behaviors of adipose tissue-derived stem cells

doi:10.3969/j.issn.2095-4344.2015.19.010

Abstract

Abstract:

BACKGROUND: Bone deficiency restricts the extensive use of oral implant restoration. How to promote stem cell migration, adhesion and proliferation so as to promote endogenous bone regeneration becomes the key of research.
OBJECTIVE: To observe the effects of different growth factors on the migration, adhesion and proliferation of rabbit adipose tissue-derived stem cells cultured in vitro and to screen the optimal combined factors.
METHODS: The inguinal adipose tissues of rabbits were cut under aseptic conditions and primary adipose tissue-derived stem cells were cultured by enzyme digestion method. Passage 3 cells were randomly divided into
five groups: transforming growth factor β1+platelet-derived growth factor AB group (group 1), platelet-derived growth factor AB+vascular endothelial growth factor group (group 2), transforming growth factor β1+vascular endothelial growth factor group (group 3), transforming growth factor β1+platelet-derived growth factor AB+vascular endothelial growth factor group (group 4), and blank control group. In vitro cell migration ability was examined by Transwell chamber. Cell proliferation and adhesion abilities were examined by cell counting kit-8 and cell adhesion assay, respectively.
RESULTS AND CONCLUSION: Results from the Transwell test showed that the migration number of rabbit adipose tissue-derived stem cells showed significant differences among the former four groups (P < 0.05), which was highest in the group 4 that significantly promoted the migration of adipose tissue-derived stem cells. In the cell adhesion assay, there were significant differences in the number of adherent cells among the former four groups (P < 0.05), and the group 3 showed the most adherent cells that dramatically improved the adhesion of adipose tissue-derived stem cells. Compared with the blank control group, the absorbance values in the groups 1-4 were significantly higher at days 1, 3, 5, 7 (P < 0.05), and the group 2 showed the highest absorbance value, indicating the combination of platelet-derived growth factor AB and vascular endothelial growth factor significantly promoted the proliferation of rabbit adipose tissue-derived stem cells.

Key words: Adipose Tissue, Stem Cells, Cell Movement, Cell Adhesion, Cell Proliferation, Transforming Growth Factor beta1, Platelet-Derived Growth Factor, Vascular Endothelial Growth Factors

CLC Number:

R394.2

Gao Jie, Wang Ming-guo, Yang Shuai, Li Xue, Yang Shi-mao, Li Xiu-mei3, Liu Jin-pan. Effects of different growth factors on biological behaviors of adipose tissue-derived stem cells[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(19): 3010-3016.

Figures/Tables 4

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