Transplanting umbilical cord blood mesenchymal stem cells in ovarian cancer chemotherapy

doi:10.3969/j.issn.2095-4344.2016.41.011

Abstract

Abstract:

BACKGROUND: Existing evidence has confirmed that umbilical cord blood mesenchymal stem cells have an effect on functional recovery of a variety of damaged cells.
OBJECTIVE: To explore the effect of umbilical cord blood mesenchymal stem cell transplantation on ovarian cancer chemotherapy.
METHODS: Sixty healthy adult female Wistar rats were randomly divided into normal control group, damage group and treatment group (n=20/group). There was no treatment in the control group, and a rat model of ovarian cancer chemotherapy damage was made in the damage group and treatment group. After successful modeling, rats in the control group were given normal saline injection via the tail vein, and those in the damage and treatment groups were given paclitaxel chemotherapy and pacligaxel chemotherapy plus umbilical cord blood mesenchymal stem cell transplantation, respectively. After transplantation of 2 weeks, mRNA and protein expressions of XAF1 and Survivin in ovarian tumor tissues were detected by RT-PCR and western blot assay, respectively. Apoptosis in ovarian cancer cells were detected using TUNEL method.
RESULTS AND CONCLUSION: Compared with the damage group, a significant up-regulation of XAF1 mRNA and protein but a remarkable down-regulation of Survivin mRNA and protein were obtained in the treatment group (P < 0.05). A severe damage to the ovarian tissues was visible in the damage group, presenting with large hemorrhage and necrosis area. This damage was markedly reduced in the treatment group. Additionally, the apoptotic rate of ovarian cancer cells was significantly higher in the treatment group than the damage group (P < 0.05). All these findings indicate that umbilical cord blood mesenchymal stem cell transplantation aids in ovarian cancer chemotherapy to promote ovarian tissue repair in rats, and XAF1 and Survivin cannot be ignored in tumor angiogenesis and ovarian cancer cell apoptosis.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Cord Blood Stem Cell Transplantation, Ovarian Neoplasms, Antineoplastic Combined Chemotherapy Protocols, Apoptosis, Tissue Engineering

CLC Number:

R394.2

Li Xia, Wang Dong-hui, Guo Liang. Transplanting umbilical cord blood mesenchymal stem cells in ovarian cancer chemotherapy[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(41): 6151-6157.

Figures/Tables 1

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