Effect of combination of neural stem cells transplantation and collagen gel scaffold on apoptosis of brain cells in rats after spinal cord injury

doi:10.3969/j.issn.2095-4344.2014.03.003

Abstract

Abstract:

BACKGROUND: Studies have shown that neural stem cells isolated from embryonic rat cerebral cortex can proliferate and differentiate into neurons, astrocytes and oligodendrocytes in collagen gels.
OBJECTIVE: To investigate the effect of neural stem cells combined with collagen gel on the apoptosis of nerve cells in the brain of rats after spinal cord injury.
METHODS: Forty-five spinal cord injury rat models were made through spinal cord hemisection and randomly divided into three groups. At 1 week after modeling, rats in the cell transplantation group were injected allogeneic neural stem cell suspension into the injured site, rats in the combination group were administered with allogeneic neural stem cells/collagen gel suspension into the injured site, and rats in the model group received no treatment.
RESULTS AND CONCLUSION: From 1 to 8 weeks after injury, the Basso, Beattie and Bresnahan (BBB) locomotion scores in the combination group were significantly higher than those in the other two groups (P < 0.05). Hematoxylin-eosin staining showed that at 1 week after transplantation, there were a few necrotic cells and Bcl-2 positive cells, but a large amount of Bax positive cells in the three groups. Then, the number of Bax- and Bcl-2-positive cells was reduced graduallyin the three groups. At 8 weeks after transplantation, the number of Bax-positive cells was significantly higher in the model group than the other two group (P < 0.05), but the number of Bcl-2-positive cells were dramatically lower (P < 0.05). Meanwhile, there were no necrotic cells in the three groups. These findings indicate that neural stem cell transplantation combined with collagen gel scaffold can arrest apoptosis of nerve cells in the brain of rats after spinal cord injury, and promote functional recovery after spinal cord injury.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

全文链接：

Key words: biocompatible materials, collagen, hydrogels, apoptosis, neural stem cells, spinal cord injury

CLC Number:

R318

Zhu Zong-hao, Shen Qiang. Effect of combination of neural stem cells transplantation and collagen gel scaffold on apoptosis of brain cells in rats after spinal cord injury[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(3): 341-347.

Figures/Tables 3

References

[1]Pierdomenico L,Bonsi L,Calvitti M,et al.Multipotent Mesenchymal stem cells with immunosuppressive activity can be easily isolated from dental pulp. Transplantation.2005; 80(6): 836-842.

[2]韩冰,付小兵.间充质干细胞的研究进展与临床应用前景[J].中国修复重建外科杂志, 2006,20(12):1257-1261.

[3]沈强,赵定麟,Gunther SCHLAG.颈髓段下行传导束诱发电位动物模型研究[J].第二军医大学学报,2000,21(7):633-635.

[4]Basso DM,Beattie MS,Bresnahan JC.A sensitive and reliable locomotor rating scale for open field testing in rats.J Neurotrauma.1995;12(1):1-21.

[5]Collins WF,Piepmeier J,Ogle E.The spinal cord injury problem: a review. Cent Nerv Syst Trauma.1986;3(4):317-331.

[6]Anderson TE, Stokes BT.Experimental models for spinal cord injury research: physical and physiological considerations.J Neurotrauma.1992;9 Suppl 1:S135-142.

[7]O’Brien MF,Lenke LG,Lou J,et al.Astrocyte response and transforming growth factor-b localization in acute spinal cord injury.Spine.1994;19:2321-2330.

[8]Williams GT,Smith CA,McCarthy NJ,et al.Apoptosis: final control point in cell biology.Trends Cell Biol.1992;2(9): 263-267.

[9]Li GL, Brodin G,Farooque M,et al.Apoptosis and expression of Bcl-2 after compression trauma to rat spinal cord.J Neuropathol Exp Neurol. 1996;55:280-289.

[10]Yong C,Arnold PM,Zoubine MN,et al.Apoptosis in cellular compartments of rat spinal cord after severe contusion injury.J Neurotrauma.1998;15(7):459-472.

[11]Brooks C,Dong Z.Regulation of mitochondrial morphological dynamics During apoptosis by Bcl-2 family proteins: a key in Bak?Cell Cycle.2007;6(24):3043-3047.

[12]Cory S,Adams JM.The Bcl2 family: regulators of the cellular life-or-death switch. Nat Rev Cancer.2002;2:647-656.

[13]Katoh K,Ikata T,Katoh S,et al.Induction and its spread of apoptosis in rat spinal cord after mechanical trauma.Neurosci Lett.1996;216(1):9-12.

[14]Crowe MJ,Bresnahan JC,Shuman SL,et al.Apoptosis and delayed degeneration after spinal cord injury in rats and monkeys. Nat Med.1997;3(1):73-76.

[15]Hains BC,Black JA,Waxman SG.Primary cortical motor neurons undergo apoptosis after axotomizing spinal cord injury.J Comp Neurol.2003;462(3):328-341.

[16]Kato H,Kanellopoulos GK,Matsuo S,et al.Neuronal apoptosis and necrosis following spinal cord ischemia in the rat.Exp Neurol. 1997;148:464-474.

[17]Mattson MP,Rychlik B,Chu C,et al.Evidence for calcium-reducing and excito-protective roles for the calcium-binding protein calbindin- D28k in cultured hippocampal neurons.Neuron.1991;6(1):41-51.

[18]Gage FH.Mammalian neural stem cell.Science. 2000;287 (5457):1433-1438.

[19]Kuan WL,Barker RA.New therapeutic approaches to Parkinson’s disease including neural transplants.Neurorehabil Neural Repair.2005;19(3):155-181.

[20]Kamei N,Tanaka N,Oishi Y.BDNF, NT-3, and NGF released from transplanted neural progenitor cells promote corticospinal axon growth in organotypic cocultures. Spine. 2007;32(12):1272-1278.

[21]张国庆,燕景锋,刘世勤.BDNF基因修饰神经干细胞移植对大鼠脊髓损伤后神经细胞凋亡的影响[J].山东医药, 2006,46(14): 22-23.

[22]Isele NB,Lee HS,Landshamer S,et al.Bone marrow stromal cells mediate protection through stimulation of PI3-K/Akt and MAPK signaling in neurons.Neurochem Int.2007;50(1): 243-250.