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    15 January 2014, Volume 18 Issue 3 Previous Issue    Next Issue
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    In vivo osteogenesis by implanting dual gene activated nanobone putty
    Zhang Yi, Sun Li, Jian Yue-kui, Hu Ru-yin, Tian Xiao-bin, Li Bo, Han Wei
    2014, 18 (3):  329-334.  doi: 10.3969/j.issn.2095-4344.2014.03.001
    Abstract ( 354 )   PDF (668KB) ( 602 )   Save

    BACKGROUND: The bone morphogenetic protein 2 (BMP2)/vascular endothelial growth factor (VEGF) dual gene activated nanobone putty has been constructed in the previous experiments.
    OBJECTIVE: To investigate the effects of osteogenesis and osteogenic gene expression in mice by implanting BMP2/VEGF dual gene activated nanobone putty.
    METHODS: Twenty-four Kunming mice (48 sides) were randomly divided into four groups. Animals in each group (12 samples) were injected different materials into the right thigh muscle pouches: nanobone putty+hBMP2/VEGF plasmid; nanobone putty+hBMP2 plasmid; blank plasmid+nanobone putty; nanobone putty only. The effects of osteogenesis were evaluated by radiography, histology and molecular biology analysis in 2, 4 weeks after operation.
    RESULTS AND CONCLUSION: Bone-like tissues were observed in groups of nanobone putty+hBMP2/VEGF plasmid and nanobone putty+hBMP2 plasmid after operation. There was apparent BMP2 and VEGF mRNA expression in group of nanobone putty+hBMP2/VEGF plasmid. Group of nanobone putty+hBMP2/VEGF plasmid was significantly better than group of nanobone putty+hBMP2 plasmid in the alkaline phosphatase levels, the speed of osteogenesisas and amount of new bone (P < 0.05). Groups of blank plasmid+nanobone putty and nanobone putty had no obvious osteogenesis performance. Either BMP2/VEGF dual gene activated nanobone putty or BMP2 gene activated nanobone putty had the osteogenic ability in vivo. And the former was significantly enhanced in the speed and quality of osteogenesis.


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    Biocompatibility of hydroxyapatite/TiO2 nanotube composites
    Zhang Hang-zhou, Sun Yu, Wang Lin, Tian Ang, Xue Xiang-xin, Bai Xi-zhuang
    2014, 18 (3):  335-340.  doi: 10.3969/j.issn.2095-4344.2014.03.002
    Abstract ( 558 )   PDF (642KB) ( 731 )   Save

    BACKGROUND: Hydroxyapatite has excellent biocompatibility, but biocompatibility of nano-hydroxyapatite/TiO2 nanotube composites is rarely reported.
    OBJECTIVE: To evaluate the biocompatibility of nano-hydroxyapatite/TiO2 nanotube composites.
    METHODS: First, the TiO2 nanotubes were fabricated on the surface of the titanium by anodic oxidation technique. Second, the nano-hydroxyapatite/TiO2 nanotube composites were fabricated by electrodeposition technique. The surface morphology of the composites was observed by scanning electron microscopy. Mouse osteoblasts MC-3T3-E1 were co-cultured with the nano-hydroxyapatite/TiO2 nanotube composites, TiO2 nanotubes and titanium, and commercially pure titanium to observe the cell adhesion, proliferation and necrosis on scaffolds.
    RESULTS AND CONCLUSION: The morphology of the TiO2 nanotubes and nano-hydroxyapatite/TiO2 nanotube composites could be controlled by altering the conditions of the anodic oxidation and electrodeposition. Under the inverted microscope, after 3 days of co-culture with TiO2 nanotubes and nano-hydroxyapatite/TiO2 nanotube composites, MC-3T3-E1 cells proliferated well with regular shape and arrangement that were superior to those on commercially pure titanium. Under scanning electron microscope, the cell were adhered and proliferated well on the surface of the TiO2 nanotubes and nano-hydroxyapatite/TiO2 nanotube composites after 3 days. Apoptosis rate of the cells was significantly reduced on the surface of nano-hydroxyapatite/TiO2 nanotube composites (7.8%) compared with TiO2 nanotubes (9.4%) and commercially pure titanium (13.5%), indicating nano-hydroxyapatite/TiO2 nanotube composites have good biocompatibility.


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    Effect of combination of neural stem cells transplantation and collagen gel scaffold on apoptosis of brain cells in rats after spinal cord injury
    Zhu Zong-hao, Shen Qiang
    2014, 18 (3):  341-347.  doi: 10.3969/j.issn.2095-4344.2014.03.003
    Abstract ( 327 )   PDF (1819KB) ( 774 )   Save

    BACKGROUND: Studies have shown that neural stem cells isolated from embryonic rat cerebral cortex can proliferate and differentiate into neurons, astrocytes and oligodendrocytes in collagen gels.
    OBJECTIVE: To investigate the effect of neural stem cells combined with collagen gel on the apoptosis of nerve cells in the brain of rats after spinal cord injury.
    METHODS: Forty-five spinal cord injury rat models were made through spinal cord hemisection and randomly divided into three groups. At 1 week after modeling, rats in the cell transplantation group were injected allogeneic neural stem cell suspension into the injured site, rats in the combination group were administered with allogeneic neural stem cells/collagen gel suspension into the injured site, and rats in the model group received no treatment.
    RESULTS AND CONCLUSION: From 1 to 8 weeks after injury, the Basso, Beattie and Bresnahan (BBB) locomotion scores in the combination group were significantly higher than those in the other two groups (P < 0.05). Hematoxylin-eosin staining showed that at 1 week after transplantation, there were a few necrotic cells and Bcl-2 positive cells, but a large amount of Bax positive cells in the three groups. Then, the number of Bax- and Bcl-2-positive cells was reduced graduallyin the three groups. At 8 weeks after transplantation, the number of Bax-positive cells was significantly higher in the model group than the other two group (P < 0.05), but the number of Bcl-2-positive cells were dramatically lower (P < 0.05). Meanwhile, there were no necrotic cells in the three groups. These findings indicate that neural stem cell transplantation combined with collagen gel scaffold can arrest apoptosis of nerve cells in the brain of rats after spinal cord injury, and promote functional recovery after  spinal cord injury.


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    Preliminary fabrication of tissue engineered veins containing valves using bone marrow mesenchymal stem cells and biodegradable scaffolds in vitro
    Liu Chi-zhuai, Yin Heng-hui, Lv Wei-ming, Zeng Chen-guang, Liu Chang, Wang Wen-jian, Quan Da-ping, Xiang Peng, Wang Shen-ming
    2014, 18 (3):  348-356.  doi: 10.3969/j.issn.2095-4344.2014.03.004
    Abstract ( 366 )   PDF (788KB) ( 674 )   Save

    BACKGROUND: Chronic venous insufficiency is a major health problem worldwide. Clinical treatments include venous valve repair and venous segment containing valve transplantation. However, these are invasive procedures, and the supply of vein containing valves is limited. Significant progress in the fields of tissue engineering and regenerative medicine has been made towards the creation of tissue engineered vascular grafts for the repair of damaged or malformed vessels. It has been reported that using tissue engineering, a tissue engineered vein containing valves constructed with self-derived endothelial cells and allogeneic acellular matrices can provide the complex physiological valve structure and mechanical stability, but this elicited an immunogenic response.
    OBJECTIVE: To create a viable and functional vein containing valves, which has the ability to grow, repair, and imitate natural tissues.
    METHODS: Bone marrow mesenchymal stem cells were obtained from Beagle dogs by density gradient centrifugation and adherence methods. Bone marrow mesenchymal stem cells were cultured in vitro. Following isolation and culture the cells were examined using flow cytometry and identified by direct induction towards the osteogenic and adipogenic lineages. We fabricated biodegradable venous scaffold containing valves using the method of injection molding combined with thermally induced phase separation. Based on the self-made cast, a three-dimensional biodegradable vein scaffold containing valves was constructed from poly(lactic-co-glycolic acid). Morphological structure was tested. Bone marrow mesenchymal stem cells were used as seed cells to be seeded onto the lumen of the tissue engineered vein scaffold containing valves in vitro and then incubated for 2 weeks.
    RESULTS AND CONCLUSION: Scanning electron microscopy images showed that the scaffold demonstrated sufficient porosity. Cultured cells expressed mesenchymal cell markers, CD44 and CD29, but did not express hematopoietic cell markers, CD34 and CD45 at the same time point. Scaffolds were nontoxic to cells and were favorable for the growth and migration of bone marrow mesenchymal stem cells. Cells attached on the surface of poly(lactic-co-glycolic acid) scaffolds formed a confluent layer after incubation. The cellular constructs were tested in vitro, and the valve leaflets were functionally capable of opening and closing when stimulated. These results suggested that the tissue engineered vein containing valves have been successfully constructed by using a three-dimensional poly(lactic-co-glycolic acid) scaffold and bone marrow mesenchymal stem cells as seed cells. Tissue engineered vein containing valves is potentially useful for the substitution and regeneration of vein valves.


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    Bionic mechanics of customized cellular bio-magnesium scaffolds for large-area defects of femoral shafts and implants for orthopaedics surgery
    Zhao Wei-min, Li Bing-zhe, Tang Liu-ding, Peter Paul Müller
    2014, 18 (3):  357-364.  doi: 10.3969/j.issn.2095-4344.2014.03.005
    Abstract ( 562 )   PDF (521KB) ( 1000 )   Save

    BACKGROUND: In 2010, the corresponding author was supported financially by German Academic Exchange Service, visiting the Department of Molecule Biotechnology, German Helmholtz Center of Infection Research, Braunschweig, Germany, creating the cooperation relation to research into the project “Fundament and Application of Biomimetic Technology for Customized Cellular Biomagnesium Scaffolds of Loaded Tissue Engineering Bones”, finishing the present work and “Investigation on the Nanosize and Zeta(ξ)-potential of Polyelectrolytes for Biomimetic Coating Technology”.
    OBJECTIVE: To focus on studying bionic mechanics between cellular scaffolds, customized for large area defects of femoral shafts, and new ingrown bones, as well as bionic mechanics between host bones and platelike implants for orthopaedics surgery, by means of elastic mechanics and stereo-quantitative metallographic analysis, and proposing the principle of bionic mechanics and the design criterion for above mentioned two types of problems, also some directions for research and development.
    METHODS: Different types of micro-volume models for two types of composites, i.e., cellular scaffolds/new-growing bones bio-composites, as well as plate-like implants/host bones layered composites were created, as well as different modulus mixing rules and mechanical stresses, i.e., stimuli, were presented, respectively. 
    RESULTS AND CONCLUSION: Under the different modulus and volume fractions of scaffold materials, the mechanical stimuli of new ingrown bones are compared: (1) except cellular tantalum, the strength of cellular metallic scaffolds/new ingrown bones composites is obviously much higher than that of original host bones, to enable resolving the problem on insufficient mechanical strengths in early period of implanting the compound between partial demineralized bone scaffolds and autogenous human bone marrow mesenchymal stem cells, as well as other organic or inorganic scaffolds for tissue engineering. (2) The less the modulus and volume fraction of materials for cellular scaffold, the greater the mechanical stimuli of new ingrown bones will be. For loaded tissue-engineered bones, particularly, for customized cellular scaffolds for large-area defects of femoral shaft, tibial shaft and fibular shaft, cellular bio-magnesium scaffolds will be the best choice. The bionic mechanics between host bones and plate-like implants for orthopaedics surgery indicates: (1) the strength and rigidity of plate-like implants will be the most important, compared with other factors, while the rise of elastic modulus will bring about the decrease of mechanical stimuli for host bones, indistinctly, if the thickness of implant plates is little, e.g., 5 mm. (2) Nonetheless, the surface macrostructure, such as fiber networks, macro-pores, and bionic(biomimetic) coating, decreasing the elastic modulus of surface structure, will be necessary to locally decrease stress shield effects and ensure the mechanical stimuli of host bones, to improve the bio-compatibility of implants surface and enhance the effect of biological fixation.


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    Effects of strontium-doped calcium polyphosphate on behavior and angiogenic growth factors expression of co-cultured osteoblasts and endothelial cells
    Peng Hong, Gu Zhi-peng, Huang Cheng-cheng, Xu Yuan-ting, Yu Xi-xun
    2014, 18 (3):  365-370.  doi: 10.3969/j.issn.2095-4344.2014.03.006
    Abstract ( 488 )   PDF (785KB) ( 718 )   Save

    BACKGROUND: Our previous studies have shown that strontium-doped calcium polyphosphate containing low-dose strontium appears to have a significant effect on angiogenesis-related behaviors of monocultured umbilical vein endothelial cells and osteoblasts.
    OBJECTIVE: To investigate the effect of strontium-doped calcium polyphosphate on angiogenesis-related behaviors of umbilical vein endothelial cells and osteoblasts co-cultured, including cell adhesion, spreading, proliferation, as well as the protein secretion of vascular endothelial growth factor and basic fibroblast growth factor from co-culture system in vitro.
    METHODS: Human umbilical vein endothelial cells and osteoblastic cells (MG63) were utilized in this study. Cells from passage 3 were used for preparation of the cell-scaffold constructs. After placed in 24-well plate at a ratio of 2:1, human umbilical vein endothelial cells and MG63 cells were seeded onto strontium-doped calcium polyphosphate, calcium polyphosphate and hydroxyapatite scaffolds and co-cultured for 7 days. The vascular endothelial growth factor and basic fibroblast growth factor protein levels were determined through a double ligand enzyme-linked immunosorbent assay. The colorimetric 3-[4,5-dimethylthiazol-2-yl]-2,5- diphenyltetrazolium bromide assay was performed to quantify the effect of scaffolds on cell proliferation.
    RESULTS AND CONCLUSION: Compared with those on calcium polyphosphate and hydroxyapatite scaffolds, cells on strontium-doped calcium polyphosphate scaffolds attached and spread better with a significantly improved cell proliferation. More importantly, the vascular endothelial growth factor and basic fibroblast growth factor expressions were significantly higher in the strontium-doped calcium polyphosphate group than the other two groups (P < 0.05), indicating strontium-doped calcium polyphosphate can up-regulate levels of vascular endothelial growth factor and basic fibroblast growth factor proteins.


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    Percutaneous kyphoplasty combined with 125I for treatment of metastatic spinal tumors 
    Zhang Wei, Shi Jian-dang, Niu Ning-kui, Zhang Jun-shan, Geng Guang-qi
    2014, 18 (3):  371-376.  doi: 10.3969/j.issn.2095-4344.2014.03.007
    Abstract ( 515 )   PDF (396KB) ( 705 )   Save

    BACKGROUND: Radiotherapy alone is not suitable for tumor-caused vertebral fractures and neurological dysfunction. In recent years, 125I radiation particles have been widely used in a variety of primary or secondary tumors and achieved good results. Percutaneous kyphoplasty can restore vertebral height efficiently, remodel spinal stability, and relieve pain.
    OBJECTIVE: To evaluate safety and effectiveness of percutaneous kyphoplasty combined with 125I in patients with metastatic spinal tumors.
    METHODS: A retrospective study was conducted to review 30 cases of metastatic spinal tumors undergoing percutaneous kyphoplasty combined with 125I from March 2011 to July 2012. Symptoms, signs, and imaging findings were collected and analyzed. All the patients had a refractoriness back pain. CT scan showed osteolytic changes in the vertebrae. The visual analogue scales, WHO standards for pain relief and Owestry disability index 
    were recorded to analyze the clinical symptoms outcome and recovery of neurological function, and the change of height in abnormal vertebrae was measured. The follow-up time was 1 day, 1 month and 6 months postoperatively.
    RESULTS AND CONCLUSION: Operations in all the 30 patients were done successfully. All patients got a conspicuous pain relief in 24 hours after operation, and nospinal injury or compression was found. There were significant differences in scores of visual analogue scales, pain levels, Owestry disability index, and the height of vertebral bodies before and after operation (P < 0.05). During postoperative follow-up of 1 and 6 months, scores of visual analogue scales, pain levels, Owestry disability index, and the height of vertebral bodies showed no difference from those at 24 hours postoperatively (P > 0.05). Bone cement leakage occurred in the anterior longitudinal ligament (n=2) and intervertebral space (n=2), and no serious complications occurred. Percutaneous kyphoplasty combined with 125I is a safe and effective way to treat metastatic spinal tumors, which can quickly ease the pain caused by spinal tumor, recover the abnormal vertebral height, reduce complications and improve life quality of patients.


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    Double-chamber stirred bioreactor improves the repaired effect of beta-tricalcium phosphate on goat knee cartilage defects
    Li Jian-wei, Zhao Hui, Zhang Xiao-qiang, Wang Lei, Xia Li-heng, Jin Dan, Wang Gang, Yu Bin
    2014, 18 (3):  377-382.  doi: 10.3969/j.issn.2095-4344.2014.03.008
    Abstract ( 396 )   PDF (1651KB) ( 696 )   Save

    BACKGROUND: A preliminary experiment developed a double-chamber stirred bioreactor which can carry out osteogenic and cartilage induction at the same time. 
    OBJECTIVE: To explore the effects of double-chamber stirred bioreactor on the repair of goat knee cartilage defects with tissue-engineered cartilage.
    METHODS: Twelve goats were selected to make bilateral femoral condyle osteochondral defects models and randomized to three groups: experimental group, implanted with the composites of β-tricalcium phosphate and bone marrow mesenchymal stem cells that were subjected to 2-week chondrogenic and osteogenic induction simultaneously in the double-chamber stirred bioreactor under mechanical stimulation; control group, implanted with the composites of β-tricalcium phosphate and bone marrow mesenchymal stem cells that were subjected to 2-week chondrogenic and osteogenic induction simultaneously in the double-chamber stirred bioreactor; blank control group, without treatment. After 12 and 24 weeks of implantation, general observation, Masson staining, II collagen immunohistochemical staining and histological scoring were performed.
    RESULTS AND CONCLUSION: In the experimental and control groups, new cartilage tissue and bone tissue were visible, but the experimental group showed better repair effects than the control group (P < 0.05). The blank control group had no cartilage formation. These findings indicate that under the mechanical stimulation by the double-chamber stirred bioreactor in vitro, the repair effect of tissue-engineered osteochondral complex on knee joint cartilage defects can be improved.


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    Repairing rabbit radial bone defects with three-dimensional tissue-engineered bone composite scaffold
    Ye Peng, Ma Li-kun, Huang Wen-liang, She Rong-feng, Tian Ren-yuan, Deng Jiang
    2014, 18 (3):  383-388.  doi: 10.3969/j.issn.2095-4344.2014.03.009
    Abstract ( 416 )   PDF (772KB) ( 825 )   Save

    BACKGROUND: Silk fibroin/chitosan/nano hydroxyapatite (SF/CS/nHA) composite scaffold constructed in preliminary experiments has good physical and chemical properties.
    OBJECTIVE: To study the capacity and mechanism of SF/CS/nHA composite scaffold for repair of rabbit radial bone defects.
    METHODS: Thirty-six New Zealand white rabbits were selected to make animal models of right radial bone defects, and then randomly divided into SF/CS/nHA group, SF/CS group and blank control group. Blank control group had no treatment after modeling. X-ray radiography, gross observation and histopathological observation were performed at 4, 8, 12, 16 weeks postoperatively.
    RESULTS AND CONCLUSION: Sixteen weeks after surgery, bone defects in the SF/CS/nHA group were completed replaced by normal bone tissue on X-ray images, and the bone marrow cavity showed complete recanalization with new bone formation; hematoxylin-eosin staining showed bone trabecula and many fusiform bone cells. In the SF/CS group, the bone mineral density in the defect area was slightly lower than that of the normal bone tissues, the bone marrow cavity was partly rehabilitated, and many chondrocytes were seen around bone cells that arranged irregularly with no bone trabecula or bone lamella. In the blank control group, the images of bone calcification were consistent with normal bone tissues, and a closed bone ununion was formed at each end; hematoxylin-eosin staining showed that the blank control group was filled by fibrous connective tissue and a small amount of bone-like tissues. SF/CS/nHA composite scaffold is better for repair of rabbit radial bone defects.

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    Carboxymethylated chitosan effects on proliferation of Schwann cells and expression of nuclear factor kappa B
    He Bin, Tao Hai-ying, Wei Ai-lin, Liu Shi-qing
    2014, 18 (3):  389-394.  doi: 10.3969/j.issn.2095-4344.2014.03.010
    Abstract ( 459 )   PDF (935KB) ( 700 )   Save

    BACKGROUND: Carboxymethylated chitosan is shown to promote some kinds of cells proliferation, but its effects on proliferation of Schwann cells need further studies.
    OBJECTIVE: To investigate the effects of carboxymethylated chitosan on proliferation of Schwann cells and expression of nuclear factor-κB in cultured Schwann cells.
    METHODS: Schwann cells from Sprague-Dawley rats at logarithmic growth phase were seeded in 96-well plates, and cultured respectively with PBS, 0, 10, 50, 100, 200, 500, 1 000 mg/L carboxymethyl chitosan for 24 hours. Cell proliferation was detected using the cell counting kit-8 assay. After trypsin digestion, Schwann cells from Sprague-Dawley rats at logarithmic growth phase were used to prepare cell suspensions, which were seeded in 6-well cell culture plates and cultured respectively with 50, 100 and 200 mg/L carboxymethyl chitosan and PBS for 24 hours. Then, 5-bromo-2-deoxyuridine, real-time PCR and western blot assay were performed.
    RESULTS AND CONCLUSION: Cell counting kit-8 and 5-bromo-2-deoxyuridine detection results showed that carboxymethyl chitosan at 50-1000 mg/L, especially at 200-500 mg/L, could promote Schwann cell proliferation. Real-time PCR and western blot results showed 50-200 mg/L carboxymethyl chitosan could promote nuclear factor κB mRNA and protein expression in Schwann cells in a dose-dependent manner, suggesting carboxymethyl chitosan can promote Schwann cell proliferation and expression of nuclear factor-κB in Schwann cells cultured in vitro.


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    Preparation and characterization of recombinant human bone morphogenetic protein-2/ poly lactic acid sustained release microspheres
    Ma Li-kun, Ye Peng, Huang Wen-liang, Tian Ren-yuan, Deng Jiang
    2014, 18 (3):  395-400.  doi: 10.3969/j.issn.2095-4344.2014.03.011
    Abstract ( 497 )   PDF (429KB) ( 683 )   Save

    BACKGROUND: Poly lactic acid as an excellent delivery has good biocompatibility.
    OBJECTIVE: To prepare recombinant human bone morphogenetic protein-2 (rhBMP-2)/poly lactic acid (PLA) sustained release microspheres, and to study its physical and chemical properties.
    METHODS: The rhBMP-2/PLA sustained release microspheres were prepared using w/o/w solvent evaporation method. Scanning electron microscopy, laser particle size, zeta potential, and swelling properties were detected. ELISA kit was utilized for measurement of encapsulation efficiency, drug-loading rate and in vitro drug release rate.
    RESULTS AND CONCLUSION: Under the scanning electron microscope, rhBMP-2/PLA sustained release microspheres were approximately circle with excellent dispersion. The uniform spheres were visible with a mean particle size of 839.6 nm. The zeta potential were (-32.93±3.74) mV. The swelling coefficient was 1.157±0.059. The drug-loading rate and encapsulation efficiency of rhBMP-2/PLA sustained release microspheres were (88.943±2.878)% and (0.026±0.001)% respectively. The drug release rate at 1 day was about 10.199%, then the drug release was relatively constant, and till 19 days, the cumulative drug release rate was 54.643%. These findings indicate that the constructed rhBMP-2/PLA sustained release microspheres meet the requirement of the Chinese Pharmacopoeia (10th edition) that the encapsulation efficiency is not less than 80% and the microspheres have a good slow-release function in vitro.


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    Coralline hydroxyapatite effects on socket site preservation after extracting maxillary incisor
    Wu Pei-ling, Nijiati•Tuerxun, Zhao Juan-tong, Zhang Xiao-qian
    2014, 18 (3):  401-405.  doi: 10.3969/j.issn.2095-4344.2014.03.012
    Abstract ( 462 )   PDF (367KB) ( 624 )   Save

    BACKGROUND: The benefits of coralline hydroxyapatite as bone graft are predominantly its safety, biocompatibility and osteoconductivity. Therefore, it can be used as a substitution biomaterial for bone in many indications clinically.
    OBJECTIVE: To observe coralline hydroxyapatite effects on maintaining alveolar ridge in the socket after extracting maxillary incisor.
    METHODS: Seventeen un-savable maxillary incisors resulting from trauma in 11 patients were extracted and at the same time coralline hydroxyapatite was implanted in socket sites. The patients were subjected to clinical observation and oral panoramic radiographs detection at postoperative 3 months.
    RESULTS AND CONCLUSION: All patients had no adverse symptoms and physical signs. X-ray findings suggested new bone formation in the implanted area with coralline hydroxyapatite at postoperative 3 and 6 months, the surrounding bone tissue had no obvious boundaries, and the height of alveolar bone was maintained. Socket preservation using coralline hydroxyapatite can effectively maintain ridge of alveolar bone following tooth extraction and can promote new bone formation.


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    Hemostasis effects of microporous polysaccharide hemostatic powder on soft tissue trauma
    Shi Yue, Du Bao-tang, He Yuan-qing, Yin Wen-jing
    2014, 18 (3):  406-411.  doi: 10.3969/j.issn.2095-4344.2014.03.013
    Abstract ( 748 )   PDF (517KB) ( 1390 )   Save

    BACKGROUND: A cross-emulsification patented product of microporous polysaccharide hemostatic powder was prepared using potatoes as raw material with independent intellectual property rights.
    OBJECTIVE: To observe hemostasis effects of microporous polysaccharide hemostatic powder on soft tissue trauma.
    METHODS: A wound, about 3 cm long and 0.5 cm deep, was made on the abdominal soft tissue of rabbits in the experimental group 1, and then microporous polysaccharide hemostatic powder, 1.0-2.0 g, was sprayed directly on the wound. In the experimental group 2, a wound, about 3.0 cm long and 1.0 cm deep, was made on the abdominal soft tissue of rabbits, and then microporous polysaccharide hemostatic powder, 1.0-2.0 g, was sprayed directly on the wound. Another rabbits with untreated wound served as controls. 

    RESULTS AND CONCLUSION: Microporous polysaccharide hemostatic powder formed a “pasty gel” covering the bleeding wound that achieved hemostatic effect. The bleeding time was (15.25±1.04) seconds in the experimental group 1 and (11.25±1.89) seconds in the experimental group 2. The marked effective and effective rates for hemostasis were 87.5% and 100%, respectively. In the control group, the bleeding time was more than 5 minutes. Hematoxylin-eosin staining showed mild muscle edema, vasodilatation of small blood vessels, and few scattered endoplasmic neutrophils infiltrated at 24 hours after treatment with microporous polysaccharide hemostatic powder; till the 7th day, inflammation subsided, mild fibrosis was visible on muscle tissue surface, the 
    hemostatic powder was completely absorbed, the wound tissue was close to the normal tissue, and there were no significant changes in muscle cells. These findings suggest that microporous polysaccharide hemostatic powder can be used for hemostasis of soft tissue trauma.


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    Alginate-chitosan microcapsule in tissue engineering research
    Jiang Heng-li, Cui Yuan-lu, Qi Xue-jie, Qi Yun, Ding Shu
    2014, 18 (3):  412-419.  doi: 10.3969/j.issn.2095-4344.2014.03.014
    Abstract ( 718 )   PDF (576KB) ( 2199 )   Save

    BACKGROUND: Alginate-chitosan microcapsule can improve the mechanical property of sodium alginate hydrogels. How to obtain the ideal sodium alginate-chitosan microcapsule and the prospect for application of the microcapsule system is the key to this study.
    OBJECTIVE: To investigate the preparation method and formation mechanism of alginate-chitosan microcapsules, to analyze several important factors affecting the strength of the microcapsule membrane, and to explore the prospects of alginate-chitosan microcapsules in immobilized cell technology, in tissue engineering and as a drug carrier.
    METHODS: The first author searched PubMed, Elsevier ScienceDirect, CNKI and Wanfang database (1987/2013) to retrieve literatures about the preparation method, formation mechanism and application prospect of alginate-chitosan microcapsules.
    RESULTS AND CONCLUSION: Sodium alginate hydrogels have many advantages in drug release and tissue engineering, but its application is limited by gel dissolution phenomena and deficiencies in its mechanical properties. Chitosan-alginate microcapsules make up for the deficiency of sodium alginate hydrogels by electrostatic interactions to form polyelectrolyte complexes. By controlling the nature of the chitosan solution--the molecular weight of chitosan, pH and concentration of chitosan solution, we can prepare the microcapsules with high film strength. Alginate-chitosan microcapsules have shown broad application prospects in immobilization technology, drug release and tissue engineering.


    中国组织工程研究杂志出版内容重点:生物材料;骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性;组织工程


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    Application of bacterial cellulose in tissue engineering
    Huang Jian-wen, Xu Yue-min
    2014, 18 (3):  420-425.  doi: 10.3969/j.issn.2095-4344.2014.03.015
    Abstract ( 612 )   PDF (362KB) ( 929 )   Save

    BACKGROUND: Because of unique physiochemical and mechanical properties and good biocompatibility and degradability, bacterial cellulose, a nanofiber, has a potential as an ideal tissue-engineered material.
    OBJECTIVE: To retrospectively analyze the application of bacterial cellulose in tissue engineering.
    METHODS: The first author researched PubMed and CNKI databases (2004/2013) to retrieve articles about application of bacterial cellulose in tissue engineering. The keywords were “bacterial cellulose; tissue engineering” in English and Chinese, respectively. Totally 48 relevant articles were analyzed.
    RESULTS AND CONCLUSION: Bacterial cellulose has unique properties, such as, high crystallinity, high mechanical strength, high water holding capacity, biodegradation, biocompatibility and three-dimensional nano network. In addition, bacterial cellulose can maintain biological activity of bioactive factors as a carrier. At last, modified bacterial cellulose can improve its mechanical and biological properties, and promote the repair and reconstruction of the tissue and organ. Currently, bacterial cellulose has been used in tissue and organ reconstruction. Bacterial cellulose as a carrier for bioactive molecules is difficult to degrade and has disadvantages of single function, which can be modified to improve its function and degradation.


    中国组织工程研究杂志出版内容重点:生物材料;骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性;组织工程


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    Demineralized bone matrix as a bone tissue engineering scaffold material 
    Chen Hai-xia, Xie Zhi-gang
    2014, 18 (3):  426-431.  doi: 10.3969/j.issn.2095-4344.2014.03.016
    Abstract ( 468 )   PDF (391KB) ( 688 )   Save

    BACKGROUND: Demineralized bone matrix as bone tissue engineering biological scaffold material is more researched currently, which has osteoinductive and osteoconductive.
    OBTECTIVE: To summarize the development of demineralized bone matrix as bone tissue engineering scaffold material and to prospect its trend.
    METHODS: The relative literatures addressing demineralized bone matrix as bone tissue engineering scaffold material published between January 1965 and May 2013 in PubMed database, Chinese Biomedical Database, Wanfang Database and FMJS database were searched by the first author. The key words were “demineralized bone matrix, scaffold material, growth factor, cells, drugs” in English and Chinese. According to the inclusion criteria, repetitive researches were excluded, and finally 34 articles were included.
    RESULTS AND CONCLUSION: Scaffold material is the key composition of tissue engineered bone, and only demineralized bone matrix has both osteoinductive and osteoconductive properties which cannot only provide space for bone repair, but also can be combined with bioactive factors, living cells, antibiotics in vitro to construct bone graft thereby promoting healing of bone defects. However, this technique is also facing some problems to be solved such as the ratio of demineralized bone matrix and various substances, disinfection, preservation of osteogenetic activity and antigenicity elimination. Sufficiently understanding demineralized bone matrix as bone tissue engineering scaffold can provide a theoretical basis for its clinical service.


    中国组织工程研究杂志出版内容重点:生物材料;骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性;组织工程


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    Development in physiological regulation and bone metabolism of magnesium
    Ma Wen-hui, Zhang Ying-ze
    2014, 18 (3):  432-439.  doi: 10.3969/j.issn.2095-4344.2014.03.017
    Abstract ( 530 )   PDF (387KB) ( 1126 )   Save

    BACKGROUND: As a lightweight metal with mechanical properties similar to natural bone, a natural ionic presence with significant functional roles in biological systems, and in vivo degradation via corrosion in the electrolytic environment of the body, magnesium (Mg)-based implants have the potential to serve as biocompatible, osteoconductive, degradable implants for load-bearing applications. On the other hand, Mg, as a critical mediator of cellular growth and activities regulation, is the second most abundant intracellular cation where it plays an important role in enzyme function and trans-membrane ion transport.
    OBJECTIVE: To retrospectively summarize the application progress, physiological role and mechanism of Mg, and to review its regulation of bone metabolism.
    METHODS: Papers addressing application, physiological and bone metabolism regulation of Mg published in core periodicals were reviewed. Studies in which Mg were used as an assistance materials, or articles regarding cell physiology in which Mg were not the main research object were excluded. Literatures concerning the regulation of bone turnover by Mg were given special attention.
    RESULTS AND CONCLUSION: Mg alloys are cytocompatible materials with adjustable mechanical and corrosion properties and show their potential as biodegradable implant materials. Moreover, Mg is abundantly distributed among the body and essential for maintaining physiological function of the cells. Mg deficiency has been associated with a number of clinical disorders including osteoporosis. But it remains to be determined what specific biochemical process is activated by Mg 2+ to regulate cellular activity and bone turnover. Further investigations of the precise mechanism are valuable to the clinical application of Mg.


    中国组织工程研究杂志出版内容重点:生物材料;骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性;组织工程


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    Progress of bone marrow mesenchymal stem cells and carriers in the treatment of femoral head necrosis
    Zhang Le-cheng, Yin Zong-sheng
    2014, 18 (3):  440-445.  doi: 10.3969/j.issn.2095-4344.2014.03.018
    Abstract ( 341 )   PDF (401KB) ( 598 )   Save

    BACKGROUND: With the development of stem cell research and regenerative medicine, a growing number of technique applications of bone marrow mesenchymal stem cells have been used for treatment of femoral head necrosis. Simple grafting and injection of bone marrow mesenchymal stem cells has a poor effect, which is combined with scaffold materials as cells carrier plays a better role.
    OBJECTIVE: To review the research progress of bone marrow mesenchymal stem cells and carriers in the treatment of femoral head necrosis and to look into the future development prospects.
    METHODS: A computer-based online search of PubMed (1989/2013) and CNKI (1989/2013) was performed for articles with the key words “osteonecrosis of the femoral head, avascularnecrosis, bone marrow mesenchymal stem cells, carrier or scaffold material” in English and Chinese, respectively. The advantages and limits of various carries and scaffolds were summarized. 
    RESULTS AND CONCLUSION: Ideal carriers can promote and induce ability of bone marrow mesenchymal stem cells to repair the femoral head. There is a variety of existing carriers, including autologous cancellous bone, allograft bone, natural biological materials, organic materials, bio-ceramic, nano-materials. Since each material has its own advantages and limitations, composite materials have begun to be used in searching for ideal carriers and scaffolds, and there are however still some issues that remain to be solved, such as lacking of unified standard of production, specification and making process; how to control and prevent the immune response and inflammation after implantation; how to ensure that the degradation rate of scaffold matches the generation rate of bone and cartilage in the femoral head; and how to identify the cytotoxicity, biocompatibility and therapeutic effects after implantation.


    中国组织工程研究杂志出版内容重点:生物材料;骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性;组织工程


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    Fabrication technologies of tissue-engineered cartilage scaffolds
    Ni Shuo, Li Peng, Zhang Wei-guo, Li Peng-sheng, Gui Hao-ran
    2014, 18 (3):  446-451.  doi: 10.3969/j.issn.2095-4344.2014.03.019
    Abstract ( 700 )   PDF (387KB) ( 928 )   Save

    BACKGROUND: Cartilage tissue engineering scaffold is a substitution for extracellular matrix, and there is a great significance on the shape and pore structure of the scaffold.
    OBJECTIVE: To retrospectively focus on the fabrication technology of three-dimensional porous cartilage tissue engineering scaffolds.
    METHODS: The first author searched PubMed, ELSEVIER SCIENCEDIRECT, Wanfang and CNKI databases (2000/2013) to retrieve relevant articles about the fabrication technology tissue-engineered cartilage scaffolds. The key words were “cartilage tissue engineering; scaffolds; fabrication” in English and Chinese, respectively.
    RESULTS AND CONCLUSION: The fabrication technologies of three-dimensional porous cartilage tissue engineering scaffolds are as follows: Phase separation/freeze-drying, hydrogels, rapid prototyping manufacturing, electrospinning, solvent casting/particulate leaching, gas foaming. The current cartilage studies have demonstrated that the pore size has a significance on the regeneration of the cartilage tissue, the pore size ranging from 100-250 μm allows for the regeneration of bone and cartilage tissue. The scaffold fabricated by the solvent casting/particulate leaching and gas foaming technology at a pore size of 100-250 μm is suitable for the bone and cartilage tissue regeneration. To obtain the adequate biological and mechanical properties, researchers usually combine a variety of methods to fabricate the cartilage tissue engineering scaffolds.


    中国组织工程研究杂志出版内容重点:生物材料;骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性;组织工程


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    Small-caliber artificial blood vessels: problems and prospects 
    Xu Zhi-wei, Tan Yan, Wu Hao, Li Wen-bin
    2014, 18 (3):  452-457.  doi: 10.3969/j.issn.2095-4344.2014.03.020
    Abstract ( 1498 )   PDF (363KB) ( 1256 )   Save

    BACKGROUND: Long-term patency rate of small-caliber artificial blood vessels is still the most important issue to be solved.
    OBJECTIVE: To review the materials and fabrication technologies of small-caliber artificial blood vessels as well as endothelialization of small-caliber artificial blood vessels.
    METHODS: A computer-based search of PubMed (2000-2013) and Wanfang database (2003-2013) was done for relevant articles using the key words of “small-caliber, vascular graft, electrospun, layer-by-layer assembly, endothelialization” in English and Chinese, respectively. A total of 125 articles were retrieved, and finally, 41 were included for further analysis.
    RESULTS AND CONCLUSION: At present, small-caliber vascular graft materials include natural biological materials, biodegradable polymers, composite materials and xenogeneic biomaterials. Preparation technologies of small-caliber artificial blood vessels mainly include electrospinning, self-assembly, rapid prototyping, and gel spinning. Numerous methods can elicit endothelialization of small-caliber artificial blood vessels, but each method has its flaws and cannot ensure long-term patency radically. With the development and diversification ofpreparation technologies and in-depth study of endothelialization of vascular prostheses, the long-term patency of small-caliber artificial blood vessels will gradually be resolved.


    中国组织工程研究杂志出版内容重点:生物材料;骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性;组织工程


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    The clinical application of the everStick high-strength fiber composite resin
    Jia Shuang, Wang De-fang
    2014, 18 (3):  458-463.  doi: 10.3969/j.issn.2095-4344.2014.03.021
    Abstract ( 544 )   PDF (374KB) ( 633 )   Save

    BACKGROUND: As a kind of fiber composite resin adopt the technology of fibre reinforcement composites, the everStick high-strength fiber composite resin has been applied in the field of dentistry prosthodontic treatment restoration.
    OBJECTIVE: To summarize the research condition about the material performance and clinical application of the everStick high-strength fiber composite resin
    METHODS: We searched the Medline, CNKI, and WanFang database in computer (from January 2000 to August 2013) using the keywords of “everStick high-strength fiber composite resin, material performance, clinical application” in English and Chinese.
    RESULTS AND CONCLUSION: The everStick high-strength fiber composite resin has the unique structure of IPN, and has the characteristics of simple operation, minimally invasive, higher strength, strong cohesive force and reversibility. Because of the minimally invasive, the everStick high-strength fiber composite resin can retain the healthy tissue of tooth with whole hog and provide the possibility for other therapeutic method because of the reversibility. At the present time, the everStick high-strength fiber composite resin has already been extended in the fields of post core, periodontal splint, orthodontic retainer, full crown, adhesive fixed bridge, fixed bridge, inlay fixed partial dentures, dental implant restoration and the base in removable partial denture. But whether the material can  be made into resin veneer and be used in color front teeth, for instance, the dental fluorosis, tooth discoloration after root canal therapy, tetracycline pigmentation teeth and hypoplasia of enamel, there is lack of comprehensive studies.


    中国组织工程研究杂志出版内容重点:生物材料;骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性;组织工程


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    Research and application of nano-hydroxyapatite/polyamide 66 biocomposites
    Wen Cong-you, Meng Chun-yang, Jiang Dian-ming
    2014, 18 (3):  464-469.  doi: 10.3969/j.issn.2095-4344.2014.03.022
    Abstract ( 392 )   PDF (357KB) ( 826 )   Save

    BACKGROUND: The nano-hydroxyapatite has obvious advantages in bone repairing and reconstruction, but its clinical application is limited for its low osteoinductive activity and poor mechanical properties. To overcome these defects, researchers, based on the bionics principles, composite nano-hydroxyapatite with inorganic or/and organic materials to get various biomimetic composite materials.
    OBJECTIVE: To review the research and application of nano-hydroxyapatite/polyamide 66 biocomposites.  
    METHODS: A computer-based search of PubMed database was undertaken with the keywords of “nano, hydroxyapatite, polyamide 66” in English to retrieve the relevant articles published from January 1987 to December 2012. Simultaneously, the relevant articles between January 1987 to December 2012 were searched in CNKI database with the key words of “nano, hydroxyapatite, polyamide 66” in Chinese. A total of 93 literatures were retrieved, and finally 56 standard literatures were included.
    RESULTS AND CONCLUSION: The nano-hydroxyapatite/polyamide 66 biocomposites have appropriate thermostability and mechanical properties as well as good biocompatibility. So far, the research and application of nano-hydroxyapatite/polyamide 66 biocomposites mainly focus on artificial vertebral body, lamina, and cage. The satisfactory clinical effects of the biocomposites show their broad clinical application prospects. However, there are still many problems to be solved. For example, there are no detailed long-term follow-up data concerning osteogenic induction and degradation. Additionally, current studies focus on the bio-safety of materials in the aspects   of cytology and histology rather than at the molecular level.


    中国组织工程研究杂志出版内容重点:生物材料;骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性;组织工程


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    Antibacterial mechanism and safety of zinc oxide
    Xiang Rong, Ding Dong-bo, Fan Liang-liang, Huang Xiao-zhong, Xia Kun
    2014, 18 (3):  470-475.  doi: 10.3969/j.issn.2095-4344.2014.03.023
    Abstract ( 1605 )   PDF (367KB) ( 3518 )   Save

    BACKGROUND: Zinc oxide has shown extraordinary antibacterial effect. But the antibacterial mechanisms of zinc oxide have not been well developed, and the safety of zinc oxide has not yet been confirmed.
    OBJECTIVE: To summarize the antibacterial mechanism and safety of zinc oxide based on the current research situation of zinc oxide.
    METHODS: The CNKI database and PubMed database (2005-01/2013-08) were used to search the related articles about zinc oxide antibacterial mechanism and safety. The retrieval keywords were “zinc oxide or ZnO, antibacterial”, “zinc oxide or ZnO, safety” both in English and Chinese.
    RESULTS AND CONCLUSTION: The antibacterial mechanism of zinc oxide is complex. Zinc oxide reveals its antibacterial power by photo catalysis, zinc ion dissolve out, and active oxygen. But the relationship between photo catalysis and active oxygen is different based on different studies. The safety of zinc oxide is not confirmed, and different studies have different results. Especially for the nano-zinc oxide, some studies have showed that the zinc oxide is toxic. All of these mean much more researches are needed to verify the safety of zinc oxide.


    中国组织工程研究杂志出版内容重点:生物材料;骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性;组织工程


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    Comparison and selective preference of preparation methods of platelet-rich gel
    Kang Jian, Yuan Wen
    2014, 18 (3):  476-481.  doi: 10.3969/j.issn.2095-4344.2014.03.024
    Abstract ( 707 )   PDF (335KB) ( 879 )   Save

    BACKGROUND: Platelet-rich plasma gel contains some kinds of growth factors, which play important roles in repair of local trauma and bone defects. Platelet-rich plasma gel is now more and more studied as tissue engineering scaffolds, but there are no unified preparation methods.
    OBJECTIVE: To summarize the studies about the preparation methods of platelet-rich plasma gel and the growth factors in platelet-rich plasma gel in recent years, and to compare different methods in order to find which one is better.
    METHODS: A online search of PubMed database from January 1980 to January 2013 and China National Knowledge Infrastructure from January 1980 to January 2013 was performed with the key words of “platelet-rich plasma, platelet-rich plasma gel, tissue engineering” in English and Chinese, respectively. The studies about detection and preparation methods of platelet-rich plasma were included and those unrelated to platelet-rich plasma were excluded. Finally, 322 articles were gained and 67 articles were included in result analysis.
    RESULTS AND CONCLUSION: All preparation methods have their advantages. We should select the suitable method according to the goal of study. With the further study of platelet-rich plasma, it will become more and more important for bone defect repair and tissue engineering research.


    中国组织工程研究杂志出版内容重点:生物材料;骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性;组织工程


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    Polyester braided wire and belt lock cable are employed for treatment of serious comminuted patellar fractures
    Shi Xin, Zhao Jian-hong, Yan Jian-jun, Liu Yi, Cui Sheng-yu, Cui Zhi-ming
    2014, 18 (3):  482-487.  doi: 10.3969/j.issn.2095-4344.2014.03.025
    Abstract ( 424 )   PDF (312KB) ( 557 )   Save

    BACKGROUND: Serious patellar comminuted fractures can be treated by a number of methods such as cerclage with tension band steel wire or steel wire, or fixation with the nickel titanium patella concentrator and absorption lines, or cerclage with 10# double-loop wire plus fixation with split-type patella claws or with the memory alloy patella concentrator. But it is difficult to achieve strongly fixed effect on patella fractures by these methods.
    OBJECTIVE: To investigate the clinical effects of suture with polyester braided wire and cerclage with belt lock cable on serious comminuted patellar fractures.
    METHODS: A retrospective analysis was performed on the clinical data of 57 patients with serious comminuted patellar fractures who received suture with polyester braided wire and cerclage with belt lock cable from January 2007 to October 2012. According to AO classification of fractures, there were nine cases of type B3, 22 cases of type C2 and 26 cases of type C3. After satisfactory reduction of comminuted patellar fractures during the operation, firstly we sewed up both sides of the expansion region of quadriceps muscle and stitched on periosteum and prepatellar tissue with thick polyester braided wire followed by patellar cerclage with belt lock cable. According to the stability of fracture blocks, tension band fixation with polyester braided wire was partially added. After the operation, the clinical effects were assessed based on the Bostman score system.
    RESULTS AND CONCLUSION: Totally 46 out of 57 cases were followed up with an average of 8 months      (4-18 months). All patients obtained bone union within 3-5 months. Wounds healed in the first stage without any infections, and fractures in all patients healed without complications such as implant loosening and skin irritation. According to the Bostman score system, 33 cases were scored excellent, 10 good and three fair. The excellent and good rate was 93.5%. It suggests that treatment of serious patellar comminuted fracture by the combination of polyester braided wire and belt lock cable leads to closely contact fracture sites and reliably fix bone fragments. It can facilitate early postoperative range of motion exercises.


    中国组织工程研究杂志出版内容重点:生物材料;骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性;组织工程


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    Man-machine interface design of a rehabilitation training equipment
    Zhang Ke-xuan, Su Zhi-jian, Gao Zhen-kui, Lin Neng-tao, Zhu Hui-yu
    2014, 18 (3):  488-492.  doi: 10.3969/j.issn.2095-4344.2014.03.026
    Abstract ( 528 )   PDF (454KB) ( 534 )   Save

    BACKGROUND: Rehabilitation training equipments play an important role in the rehabilitation treatment. Because of poor muscle strength and joint mobility in patients, we must guarantee the safety of rehabilitation training equipments.
    OBJECTIVE: To design a new suitable man-machine interface that ensures patients can use rehabilitation equipments and even parts of fitness equipments safely.
    METHODS: Through user experience research, we found the flaws of the existing rehabilitation equipment. Depending on the principles of ergonomics, we designed a new man-machine interface for upper limb exercise through survey and computer-aided design.
    RESULTS AND CONCLUSION: The new man-machine interface program achieves the rapid wear and discharge between patients and rehabilitation training equipment, and importantly, it can automatically separate people from the equipment when the patient's body discomforts or equipment failure appears. What’s more, this man-machine interface can be promoted to other fitness equipments. As a result, rehabilitation training for patients will be more convenient.


    中国组织工程研究杂志出版内容重点:生物材料;骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性;组织工程


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