Regulatory function of microRNA-182 in apoptosis of nucleus pulposus cells

doi:10.3969/j.issn.2095-4344.2016.42.010

Abstract

Abstract:

BACKGROUND: Previous study has found that hsa-miR-182 is probably related to the apoptosis-related genes such as cytochrome C (Cycs C) and calcineurin subunit CnB (PPP3R1) in nucleus pulposus cells.
OBJECTIVE: To determine whether miR-182 plays a regulatory role in nucleus pulposus cell apoptosis by detecting the relative gene expression levels after transfecting miR-182 with Cycs C and PPP3R1 into nucleus pulposus cells via plasmid delivery.
METHODS: After a bioinformatics prediction about miR-182, miR-182 and target genes were transfected into the nucleus pulposus cells, and at the same time, blank control group was established. Then the expression levels of the target genes were detected through cell lysis.
RESULTS AND CONCLUSION: miR-182 significantly inhibited the expression of Cycs C in nucleus pulposus cells compared with the blank control group (P < 0.05). Compared with the blank control group, miR-182 made no inhibitory effect on the expression of PPP3R1. These findings suggest that miR-182 may play a regulatory part in nucleus pulposus cell apoptosis by inhibiting the expression of Cycs C.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: MicroRNAs, Cytochromes C, Apoptosis, Tissue Engineering

CLC Number:

R318

Qiu Xiao-wen, He Xi-jing, Wang Dong, Li Hao-peng, Zhao Bo. Regulatory function of microRNA-182 in apoptosis of nucleus pulposus cells[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(42): 6296-6301.

Figures/Tables 3

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