Construction of mouse models of experimental allergic rhinitis and the correlation with Th1/Th2 imbalance

doi:10.3969/j.issn.2095-4344.2015.40.022

Abstract

Abstract:

BACKGROUND: Atopic individuals predispose to allergic rhinitis after contacting with an allergen, which is mainly released by IgE-mediated histamine. Allergic rhinitis is a kind of non-infectious inflammatory disease that involves a variety of immune cells and cytokines and is related to Th1 and Th2 immune imbalance. Interferon γ is a cytokine secreted by Th1 cells, while interleukin-4 is a cytokine secreted by Th2 cells.

OBJECTIVE: To establish a stable 129Sv mouse model of allergic rhinitis, so as to lay the foundation of establishing gene knockout 129Sv mouse models of allergic rhinitis, and to observe the concentration variation of IgE, interleukin-4 and interferon γ.

METHODS: Twenty-four mice were randomly divided into 2 groups: model and control groups. Mice in the model group were sensitized by intraperitoneal injection of ovalbumin to establish mouse models of allergic rhinitis. Mice in the control group were subjected to intraperitoneal injection of PBS. After successful modeling, pathological changes of nasal eosinophils and plasmocyte infiltration were evaluated by nasal mucosa staining. Interleukin 4 and interferon γ levels and ovalbumin-specific IgE antibody concentration were detected by enzyme-linked immunosorbent assay.

RESULTS AND CONCLUSION: Enzyme-linked immunosorbent assay showed that compared with the control group, serum ovalbumin-IgE and interleukin-4 concentrations were significantly increased, and Interferon γ concentration was significantly lower in the model group (P < 0.05). Hematoxylin-eosin staining results showed that cilia lodging and submucosal serous gland hyperplasia, eosinophils and plasmocyte infiltration were obvious. These results confirm that a mouse model of allergic rhinitis was successfully constructed in this study and the pathogenesis of allergic rhinitis was related to Th1/Th2 imbalance.

中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

Key words: Tissue Engineering, Models, Animal, Rhinitis, Mice

CLC Number:

R318

Li Lin-ge, Feng Juan, Hu Bin, Shou Xi, Zhang Chun, Zhang Yu, Zhang Hua. Construction of mouse models of experimental allergic rhinitis and the correlation with Th1/Th2 imbalance[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(40): 6515-6519.

Figures/Tables 2

References

[1] Kay AB. Advances in immunology-Allergy and allergic diseases-First of two parts. N Engl J Med. 2001;344(1):30-37.

[2] Cui Z, Zhang H. Analysis of HLA-DRB1 polymorphism for patienets with allergic rhinitis of Han people in Xinjiang. Xinjiang Yixueyuan Xuebaio. 2010;3:011.
[3] Sánchez-Velasco P, Mendizábal L, Antón EM, et al. Association ofhypersensitv tothe nematode Anisakis simplex with HLA class II DRB1* 1502-DQB1* 0601 haplotype. Human Immunol. 2000;61(3):314-319.
[4] Qi M, Wei H, Zhu Q, et al. Association between atopy for PlatanusAcerifolia pollen and HLA-DRB1 alleles. Linchuang Jianyan Zazhi. 2006;6:002.
[5] 邢志敏,于德林.蒿属花粉过敏变应性鼻炎与HLA-DRB基因多态性的相关性研究[J].临床耳鼻咽喉科杂志,2001,15(5):199-201.
[6] Yunlian C, Yangbing YYX. Analysis of HLA-DQB1 polymorphism for patienets with allergic rhinitis of Uygur and Han people in Xinjiang. Linchuag Erbiyanhouke Zazhi. 2011; 14:008.
[7] Munthe-Kaas MC, Carlsen KL, Carlsen KH, et al. HLADr-Dq haplotypes and the TNFA-308 polymorphism: associations with asthma and allergy. Allergy. 2007;62(9):991-998.
[8] Baraniuk JN. Pathogenesis of allergic rhinitis. J Allergy Clin Immunol. 1997;99(2):S763-S772.
[9] Li Y, Yang G, Jia Y, et al. Genetic polymorphism in the second exon of HLA-DRB1 in cervical cancer. Clin Oncol Cancer Res. 2010;7(1):27-32.
[10] 刘恩岐,尹海林,顾为望.医学实验动物学[J].遗传,2008,30(7): 918-918.
[11] Gerlai R. Zebra fish: an uncharted behavior genetic model. Behav Genet. 2003;33(5):461-468.
[12] Fülöp AK, Földes A, Buzás E, et al. Hyperleptinemia,visceral adiposity,and decreased glucose tolerance in mice with a targeted disruption of the histidine decarboxylasegene. Endocrinology. 2003;144(10):4306-4314.
[13] 吉晓滨,邓家德,王磊,等.变应性鼻炎红细胞免疫功能的实验观察[J].中国耳鼻咽喉头颈外科,2007,13(11):788-790.
[14] 田理,贾德蓉,刘敬.力克敏喷剂治疗过敏性鼻炎实验研究[J].中医耳鼻喉科学研究杂志,2007,6(3):28-30.
[15] 于海玲,孙连平,曲香芝,等.通鼻胶囊对急性炎症和免疫性炎症的影响[J].延边大学医学学报,2006,29(1):27-29.
[16] 杨军,邓秋,周芸,等.实验性变应性鼻炎中鼻黏膜组胺的测定[J].首都医科大学学报,2006,27(1):109-112.
[17] 佘文煜,董震.普仑司特对实验性变应性鼻炎鼻黏膜组织重塑的影响[J].中华耳鼻咽喉头颈外科杂志,2006,41(7):483-487.
[18] 王向东,韩德民,周兵,等.变应性鼻炎白细胞祖代细胞和相关细胞因子的检测及意义[J].耳鼻咽喉:头颈外科,2004,10(2):95-97.
[19] 张志明,孙树岩,季文樾.真菌性抗原致变应性鼻炎豚鼠模型建立[J].中国耳鼻咽喉头颈外科,2005,12(5):313-315.
[20] 余洪猛,文三立.豚草花粉变应原致过敏性鼻炎豚鼠模型的建立[J].上海实验动物科学,2001,21(4):212-214.
[21] Kumar RK, Herbert C, Foster PS. The“classical”ovalbumin challenge model ofasthma in mice. Curr Drug Target. 2008; 9(6):485-494.
[22] Shirakawa T, Li A, Dubowitz M, et al. Association between atopy and variantsof the β subunit of the high-affinity immunoglobulin E receptor. Nat Genet. 1994;7(2):125-130.
[23] Stern ME, Siemasko K, Gao J, et al. Role of interferon-γ in a mouse model of allergic conjunctivitis. Invest Ophthalmol Vis Sci. 2005;46(9):3239-3246.
[24] 林志荣,王维刚,严惠敏,等.卵清蛋白诱导129Sv小鼠建立哮喘模型的方法[J].中国细胞生物学学报,2011,33(9):964-968.
[25] 肖淑华,刘阳阳,魏连海,等.肥大细胞的研究进展[J].生理科学进展,2011,42(2):104-107.
[26] 洪娟,黄建明.嗜酸性粒细胞趋化因子与鼻变应性疾病[J].国际耳鼻喉头颈外科杂志,2006,30(4):251-254.
[27] Ismaïl A, Bousaffara R, Kaziza J, et al. Polymorphism in transporter antigen peptides gene (TAP1) associated with atopy in Tunisians. J Allergy Clin Immunol. 1997;99(2): 216-223.
[28] Moffatt MF, Traherne JA, Abecasis GR, et al. Single nucleotide polymorphism and linkage disequilibrium within the TCR alpha/delta locus. Hum Mol Genet. 2009;9(101)1-9.
[29] Kim JJ, Kim MS, Lee JH, et al. STAT6 gene polymorphisms in allergic rhinitis.Genomics Inform. 2004;2:126-130.
[30] Chae S, Park Y, Li C, et al. Analysis of the variations in IL-28RA gene and their association with allergic rhinitis. Exp Mol Med. 2006;38(3):302.
[31] Lee SM, Gao B, Dahl M, et al. Decreased FoxP3 gene expression in the nasal secretions from patients with allergic rhinitis. Otolaryngol Head Neck Surg. 2009;140(2):197-201.
[32] Sogut A, Yilmaz O, Kirmaz C, et al. Regulatory-T, T-helper1, and T-helper2 cell differentiation in nasal mucosa of allergic rhinitis with olive pollen sensitivity. Int Arch Allergy Immunol. 2011;157(4):349-353.