Stability of a rat model of type 2 diabetic mellitus induced by streptozotocin combined with high-fat feeding and its eye disease characteristics

doi:10.3969/j.issn.2095-4344.2015.27.023

Abstract

Abstract:

BACKGROUND: Streptozotocin-induced diabetic ophthalmopathy is commonly used in animal models, but the pathological changes are local that mainly emphasize on the retina. Little evidence is found about the animal models of the pathology of diabetic ophthalmopathy.
OBJECTIVE: To investigate the long-term stability of type 2 diabetic mellitus rat models induced by streptozotocin combined with high-fat feeding and to observe the characteristics of eye disease.
METHODS: Rats were randomly divided into control group and diabetes group. Control group was given normal feeding, while diabetes group was given intraperitoneal injection of streptozotocin combined with high-fat feeding to establish diabetic models.
RESULTS AND CONCLUSION: Compared with the control group, at 1 month after modeling, the fasting blood glucose levels increased, and the insulin sensitivity index decreased in the diabetic group (P < 0.05). Evans blue staining results showed that, at 3 months after modeling, retinal cell lesions exacerbated in the diabetic group; at 5 months after modeling, retinal blood vessels traveled in circuity and disorderly, accompanied by the leakage in
the diabetic group, Evans blue content in the retina increased as the time after modeling went by (P < 0.05). Under transmission electron microscopy, at 5 months after modeling, the eye lenses in the diabetes group were flocculent pieces, which were the typical characteristics of cataract. Experimental findings indicate that the rat model of type 2 diabetic mellitus induced by streptozotocin combined with high-fat feeding has a long-term stability, and its eye changes are consistent with the characteristics of diabetic ophthalmopathy. Therefore, it is an ideal animal model for diabetic ophthalmopathy.

中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

Key words: Tissue Engineering, Diabetes Mellitus, Animal Model, Retina

CLC Number:

R318

Yu Yang, Zheng Shi-lei, Liu Xue-zheng. Stability of a rat model of type 2 diabetic mellitus induced by streptozotocin combined with high-fat feeding and its eye disease characteristics[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(27): 4389-4393.

Figures/Tables 2

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