Effect of oxygen-glucose deprivation on the proliferation, differentiation and apoptosis of neural stem cells

doi:10.3969/j.issn.2095-4344.2015.06.010

Abstract

Abstract:

BACKGROUND: Hypoxia is one of the main pathological factors of nervous system diseases, which plays numerous regulatory roles in survival, migration, differentiation, apoptosis of endogenous neural stem cells and exogenous neural stem cells after transplantation.
OBJECTIVE: To systematically observe the effects of oxygen-glucose deprivation on the proliferation, differentiation and apoptosis of neural stem cells.
METHODS: Neural stem cells were isolated from the olfactory bulb of newborn Kunming mice, to establish oxygen-glucose deprivation models. Immunofluorescence technique was used to determine the differentiation ability of neural stem cells subject to oxygen-glucose deprivation, MTT method was used to measure the proliferative ability of neural stem cells under normoxic conditions after oxygen-glucose deprivation for 24 hours and 48 hours, and Hochest 33258 staining was performed to detect the apoptosis of neural stem cells after oxygen-glucose deprivation for 24 hours and 48 hours.
RESULTS AND CONCLUSION: Passage 4 neural stem cells were positive for CD133. Compared with the normoxic group, MTT showed that the proliferation of neural stem cells was significantly lower in the oxygen-glucose deprivation group (P < 0.05), moreover, the proliferative ability of neural stem cells was lower in
the 48-hour oxygen-glucose deprivation group than the 24-hour oxygen-glucose deprivation group (P < 0.05). The number of GFAP and β-Tubulin III positive cells was significantly lower in the 48-hour oxygen-glucose deprivation group than the normoxic group (P < 0.05). Apoptotic rate was significantly increased after oxygen glucose deprivation for 48 hours as compared with oxygen-glucose deprivation for 24 hours (P < 0.01) and normoxic group (P < 0.01). These results indicate that oxygen-glucose deprivation have adverse effects on neural stem cell proliferation, differentiation and apoptosis, and its impact depends on the concentration and time of hypoxia as well as the ability of tolerance to hypoxia.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: Anoxia, Neural Stem Cells, Olfactory Bulb, Apoptosis

CLC Number:

Ma Jun-ning,Gao Jun-wei, Hou Bo-ru, Ren Hai-jun, Chen Si-hua, Liu Ji-xing, Yan Gui-zhong. Effect of oxygen-glucose deprivation on the proliferation, differentiation and apoptosis of neural stem cells[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(6): 876-882.

Figures/Tables 3

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