Effects of resina draconis on Toll-like receptor-4/nuclear factor-kappaB and dendritic cell phenotypes in colitis rats

doi:10.3969/j.issn.2095-4344.2015.05.017

Abstract

Abstract:

BACKGROUND: Dendritic cells can regulate the immunological reaction in the intestinal tract, this functional deficit may induce inflammatory bowel disease. Toll-like receptor-4/nuclear factor-κB pathway is highly involved in this reaction.
OBJECTIVE: To establish experimental colitis model in rats, to observe effects of resina draconis on dendritic cells and Toll-like receptor-4/nuclear factor-κB expression in rats with experimental colitis, and to explore its action mechanism.
METHODS: A total of 44 male Sprague-Dawley rats were randomly assigned to four groups (n = 11): blank control group, model group, resina draconis group, 5-aminosalicylic acid treatment group. With the exception of blank control group, 2,4,6-trinitrobenzenesulfonic acid-induced ulcerative colitis models were established in the model group, resina draconis group and 5-aminosalicylic acid treatment group. After the models were successfully established, the rats in the resina draconis and 5-aminosalicylic acid treatment groups were intragastrically treated with resina draconis [(0.75 g(kg•d)] and 5-aminosalicylic acid [100 mg(kg•d)] respectively for 10 days.
RESULTS AND CONCLUSION: Disease activity index, macroscopic colonic damage score and histopathological score were significantly decreased in the resina draconis group compared with the model group (P < 0.05). Symptoms and tissue damages were obviously lessened in the 5-aminosalicylic acid treatment and resina draconis groups compared with the model group. Expression rates of CD80 and CD86, as well as expression levels of Toll-like receptor-4 and nuclear factor-κB were significantly higher in the model group compared with the blank control group, resina draconis group and 5-aminosalicylic acid treatment group (P < 0.05, P < 0.01). Toll-like receptor-4 and nuclear factor-κB expression was significantly lower in the resina draconis group than that in the 5-aminosalicylic acid treatment group. Experimental findings indicate that, resina draconis can partially relieve experimental colitis symptoms in rats and effectively inhibit the activation of dendritic cells in the mesenteric lymph node. Resina draconis can relieve enteric inflammatory reaction by suppressing the expression of Toll-like receptor-4 and nuclear factor-κB in rats.

中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

全文链接：

Key words: Tissue Engineering, Colitis, Trinitrobenzenesulfonic Acid, Mesentery

CLC Number:

R318

Li Nan, Wang Xue-ming, Ji Yang, Shi Yu-ling, Wang Xin, Li Na, Su Li, Sha Li-na. Effects of resina draconis on Toll-like receptor-4/nuclear factor-kappaB and dendritic cell phenotypes in colitis rats[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(5): 752-758.

Figures/Tables 7

References

[1] Carrera E, Manzano R, Garrido E. Efficacy of the vaccination in inflammatory bowel disease. World J Gastroenterol. 2013;19(9):1349-1353.
[2] Xavier RJ, Podolsky DK. Unravelling the pathogenesis of inflammatory bowel disease. Nature. 448(7152):427-434.
[3] Diamante G, Dikstein R. Transcriptional control by NF-κB: elongation in focus. Biochim Biophysics Acta. 2013;1829 (9):937-945.
[4] Lzcue A, Coombes JL, Powrie F. Regulatory T cells suppress systemic and mucosal immune activation to control intestinal inflammation. Immunol Rev. 2006;212:256-271.
[5] Chen S, Zeng ZP, Li HZ, et al. Relationship between plasma level of leptin and the expression of leptin receptor in human adrenal tissues and tumors. Zhonghua Yi Xue Za Zhi. 2010;19;90(38):2667-2670.
[6] Petvises SO, Neill HC. Characterisation of dendritic cells arising from progenitors endogenous to murine spleen. PLoS One. 2014;14;9(2):e88311.
[7] Li N, Wang XM, Wu K, et al. Influence of Compound Xuejie Enema on the Blood Rheology of Chronic Nonspecific Ulcerative Colitis. Zhongguo Quanke Yixue. 2006;9(24): 2073-2074.
[8] Wang XM, Wu K, Shi YL, et al. Preparation and Clinical Application of Compound Xuejie enema. Zhongguo Yiyuan Yaoxue Zazhi. 2005;25(3):281.
[9] Sobczak M, Zakraewski, Zakrzewski PK, et al. Anti-inflammatory and antinociceptive action of the dimeric enkephalin peptide biphalin in the mouse model of colitis: New potential treatment of abdominal pain associated with inflammatory bowel diseases. Peptides. 2014;60:102-106.
[10] Cooper HS, Murthy SN, Shah RS, et al. Clinicopathologic study of dextran sulfate sodium experimental murine colitis. Lab Invest. 1993;69(2):238-249.
[11] Tu P, Tsaip L, Lin Y, et al. Expression profile of Toll-like receptor mRNA in pigs co-infected with porcine reproductive and respiratory syndrome virus and porcine circovirus type 2. Res Vet Sci. 2014;14(3):1-9.
[12] Brenan M, Rees DJ. Sequence analysis or rat integrin alphaE1 and alphaE2 subunits: tissue expression reveals phenotypic similarities between intraepithelial lymphocytes and dendritic cells in lymph. Eur J Immunol. 2000;30(6): 1527-1537.
[13] Tanaka K. Expression of Toll-like receptors in the intestinal mucosa of patients with inflammatory bowel disease. Expert Rev Gastroenterol Hepatol. 2008;2(2):193-196.
[14] Arasaradnam R, Nwokolo CU. Epiphenomenon of telomere lengths lessons from ulcerative colitis. Gut. 2012;61(10):1516.
[15] Pulli B, Ali M, Forghani R, et al. Measuring myeloperoxidase activity in biological samples. PLoS One. 2013;8(7):1-10.
[16] Zhang X, Ma J, Xu M, et al. Imbalance between CD205 and CD80/CD86 in dendritic cells in patients with immune thrombocytopenia. Thromb Res. 2014;19(14):1-10.
[17] Haider J, Marisavljevic D, Stanisavljevicn, et al. BCL10 aberations and NF-kappa B activation involving p65 are absent or rare in primary gastric MALT lymphoma. Vojnosanit Preql. 2014;7(11):1040-1044.
[18] Dimitrakopoulos GN, Dimtrakopoulou K, Maraziotis IA, et al. Supervised method for construction of microRNA-mRNA networks: application in cardiac tissue aging dataset. Conf Proc IEEE Eng Med Biol Soc. 2014;26(30):318-321.
[19] Wang H, Ouyang Q, Hu RW. Establishment of rat colitis model induced by trinitrobenzenesulfonic acid. Weichang Bingxue. 2001;6(1):7-10.
[20] Yang XY, Yao L. Pharmacological action and clinical experience of dragon’s blood. Heilongjiang Yiyao. 2011; 24(2): 265-266.
[21] Xia PF, Ding LY. The research progress of dragon's blood. Hebei Zhongyiyao Xuebao. 2006;21(1):40-43.
[22] Li N, Wang XM, Gao LC, et al. Experimental study of resina draconis in protecting intestinal mucous membrane barrier in burned rats. Zhongguo Zhongxuyi Jiehe Xiaohua Zazhi. 2010;18(6):351-354.
[23] Li N, Wang XM, Wu K, et al. Experimental study of resina draconis on rats with ulcerative colitisin. Zhongguo Zhongxiyi Jiehe Xiaohua Yiyuan. 2007;15(6):377-379.
[24] Creticos PS. Advances in synthetic peptide immuno- regulatory epitopes. World Allergy Organ J. 2014;7(1):30.
[25] Wang J, Wang X, Hussain S, et al. Distinct roles of different NF-κB subunit s in regulating inflammatory and T cell stimulatory gene expression in dendritic cells. J Immunol. 2007;178(11):6777-6788.
[26] Zhou HG, Chen HB, Wu MH. Viewing from the toll-like receptor/nuclear factor-κB signaling pathway to explore the immunomodulatory mechanism of Chinese drugs. Zhongguo Zhongxiyi Jiehe Zazhi. 2010;30(8):884-888.
[27] Yu S, Tang S, Mayer GD, et al. Interactive effects of ultraviolet-B radiation and pesticide exposure on DNA photo-adduct accumulation and expression of DNA damage and repair genes in Xenopus laevis embryos. Aquat Toxicol. 2014;12(4):256-266.
[28] Tan W, Huang W, Gu X, et al. IL-17F/IL-17R interaction stimulates granulopoiesis in mice. Exp Hematol. 2008; 36(11): 1417-1427.
[29] Ahmed S, Shaffer A, Geddes T, et al. Evaluation of optimal RNA extraction method from human carotid atherosclerotic plaque. Cardiovasc Pathol. 2014;5(14):1-9.