In vitro constructing a of three-dimensional hepatocarcinoma model for drug screening

doi:10.3969/j.issn.2095-4344.2014.27.020

Abstract

Abstract:

BACKGROUND: In vitro construction of three-dimensional (3D) tumor model has been growing to substitute two-dimensional (2D) tumor model for drug screening.
OBJECTIVE: To develop 3D hepatocarcinoma model for the sensitive study of antitumor drugs.
METHODS: Taking HepG2 as cell model, hydrogel scaffold was fabricated with chitosan/collagen to construct in vitro 3D hepatocarcinoma model. The 3D hepatocarcinoma aggregates were characterized regarding to the morphology, growth and cytoskeleton distribution and so on. Finally, the sensitive assay of in vitro 3D hepatocarcinoma model to the clinical antitumor drugs was studied with 2D hepatocarcinoma model as control.
RESULTS AND CONCLUSION: (1) HepG2 cells in chitosan/collagen scaffold grew to form 3D cell aggregates after 10-day culture. (2) Although the growth rate of HepG2 cells in chitosan/collagen scaffold was slightly slower than that of cells in 2D culture, the HepG2 cell viability of 3D culture could be maintained longer. (3) It was found that the fibrin skeleton of HepG2 cells in chitosan/collagen scaffold rearranged and displayed structural similarity to in vivo hepatic tissue. (4) The sensitivity of in vitro 3D hepatocarcinoma model to the clinical antitumor drugs was significantly lower than that of 2D cells. In conclusion, the in vitro 3D hepatocarcinoma model developed in chitosan/collagen scaffold provided cytoskeleton structure closer to in vivo hepatic tissue, which is potential system for in vitro drug screening.

中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

全文链接：

Key words: collagen, chitosan, hepatocytes, antineoplastic combined chemotherapy protocols

CLC Number:

R318

Liu Jin-song. In vitro constructing a of three-dimensional hepatocarcinoma model for drug screening[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(27): 4389-4394.

Figures/Tables 4

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