Feasibility of in situ eyelid reconstruction in guinea pigs using human umbilical cord jelly

doi:10.3969/j.issn.2095-4344.2014.21.019

Abstract

Abstract:

BACKGROUND: Healthy human umbilical cord jelly is a mucous connective tissue without vessels and has been used in eye plastic operation because of its elasticity and toughness. It contains lysozyme, placental globulin, hyaluronic acid enzyme, AMP antibody and complement, and also contains a lot of mesenchymal stem cells, so it is not prone to infection and rejection.
OBJECTIVE: To observe the histocompatibility and histopathological changes of human umbilical cord jelly as a tarsal substitute transplanted for eyelid reconstruction in guinea pigs.
METHODS: The mucous connective tissue of healthy neonate umbilical cord jelly was made into tissue blocks at even thickness. Models of tarsal defects were established in guinea pigs, and then the mucous connective tissue of healthy neonate umbilical cord jelly was implanted. Samples of implanted materials were collected for histological examination at 1, 2, 3 weeks postoperation under light microscope.
RESULTS AND CONCLUSION: There were no obvious rejection, infection and eyelid deformation observed, the
corneas of all the animals were clear, corneal epithelial shedding did not occur, and the eyelid could move normally. One week after implantation, there was no infection and rejection on the conjunctiva and the incision of the eyelid, the eyelid could move normally, and partial inflammatory cells were observed between the human umbilical cord jelly and the muscle of the eyelid with microscopy. Two weeks after implantation, there was no infection and rejection on the conjunctiva and the incision of the eyelid, the cornea was clear, the eyelid and eye could move normally, and no symblepharon occurred; umbilical cord jelly showed the tendency of absorption, and the inflammatory cells were reduced at 2 weeks after implantation. Three weeks after implantation, the incision of the conjunctiva healed well, the cornea was clear; there was no difference in the eyelid form and movement between the two eyes; the umbilical cord jelly was absorbed partially, normal fibrous tissues formed and there were no inflammatory cells. With low immunogenicity, human umbilical cord jelly can guide the growth of new collagen and serve as an ideal tarsal substitute.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

全文链接：

Key words: umbilical cord, eyelids, histocompatibility

CLC Number:

R318

Wang Ling, Fang Yan-ning, Bu Xian-min, Chen Chao, Liu Jun . Feasibility of in situ eyelid reconstruction in guinea pigs using human umbilical cord jelly [J]. Chinese Journal of Tissue Engineering Research, 2014, 18(21): 3386-3390.

Figures/Tables 2

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