Histocompatibility of
poly(L-lactide-co-ε-caprolactone)/cross-linked polyvinylpyrrolidone ureteral
stent grafted into the rat bladder

doi:10.3969/j.issn.2095-4344.2214

Abstract

Abstract:

BACKGROUND: With the development of tissue engineering, materials science, and biomechanics, developing new biodegradable ureteral stent has become an issue of concern. The ureteral stent with poly(L-lactide-co-ε-caprolactone)/crosslinked polypyrrolidone was prepared.

OBJECTIVE: To explore the histocompatibility of the ureteral stent grafted into the bladder of Sprague-Dawley rats.

METHODS: Sixty male Sprague-Dawley rats (provided by Laboratory Animal Center of Sichuan Academy of Traditional Chinese Medicine) were randomly divided into four groups, each group containing 15 rats. The sham operation group was directly sutured after opening a small incision on the outside of the bladder, and no material was implanted. The other three groups were implanted with polyurethane ureteral stent (control group), poly(L-lactide-co-ε-caprolactone)/8% cross-linked polyvinylpyrrolidone ureter stent (experiment group 1), poly(L-lactide ε- caprolactone)/5% cross-linked polyvinylpyrrolidone ureteral stent (experiment group 2) after opening a small incision on the outer side of the bladder, followed by suturing the incision. At 4, 8, and 16 weeks after operation, the local anatomy of the bladder was observed. The histocompatibility of the materials in each group was observed by hematoxylin-eosin staining. The study was approved by the Ethical Committee of Laboratory Animal Analysis and Testing Center of West China School of Public Health, Sichuan University.

RESULTS AND CONCLUSION: (1) Gross observation: at 4, 8 and 16 weeks postoperatively, different degrees of chronic inflammation reaction occurred in the control group and experimental group 1. In the experiment group 2, chronic inflammatory reaction appeared at 4 weeks postoperatively. The stone formation rate in the control group and experimental group 1 was significantly higher than that in the sham operation group (P < 0.05) at 4 and 16 weeks postoperatively, which showed no significant difference at 8 weeks postoperatively among groups (P > 0.05). The calculus formation rate in the experimental group 2 was significantly higher than that in the sham operation group only at 4 weeks postoperatively, and had no significant difference at other time points (P > 0.05). (2) Pathological observation: different degrees of foreign body in the outer membrane, inflammatory reaction and diffuse hyperplasia of the mucosa were found in the control group, experimental groups 1 and 2 at different time points postoperatively. There was no significant difference in the rate of diffuse hyperplasia of the mucosa at different time points postoperatively among groups (P > 0.05), but higher than that in the sham operation group (P < 0.05). (3) These results indicate that the calculus formation rate and bladder histological reaction of poly(L-lactide-co-ε-caprolactone)/cross-linked polyvinylpyrrolidone ureter stent are comparable with those of commercial ureteral stents, especially the addition of 5% cross-linked polyvinylpyrrolidone has better histocompatibility.

Key words: biomaterials, histocompatibility, L-Lactide, ε-caprolactone, cross-linked polyvinylpyrrolidone, ureteral stent tube, bladder, pathology

CLC Number:

Tang Mengmeng, Chen Hechun, Xie Hongchen, Zhang Yu, Tan Xiaoshuang, Sun Yixuan, Huang Yina. Histocompatibility of poly(L-lactide-co-ε-caprolactone)/cross-linked polyvinylpyrrolidone ureteral stent grafted into the rat bladder[J]. Chinese Journal of Tissue Engineering Research, 2020, 24(4): 583-588.

Figures/Tables 7

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