Osteoblast proliferation on the surface of titanium alloy modified by diamond-like carbon/casein phosphopeptide composite film

doi:10.3969/j.issn.2095-4344.2013.08.007

Abstract

Abstract:

BACKGROUND: Titanium alloy surface modification with diamond-like carbon film can improve the friction and corrosion resistance of titanium alloy, but it is lack of biological activity. So fixing biological protein molecules on the diamond-like carbon surface is a kind of new biological chemical modification method.
OBJECTIVE: To observe the proliferation and adhesion of osteoblasts on the titanium alloy surface modification with diamond-like carbon/casein phosphopeptide composite film.
METHODS: Using unbalanced magnetron sputtering and multi-step assembly method diamond-like carbon/ casein phosphopeptide composite film was prepared on titanium alloy surface. The osteoblast suspension at logarithmic growth phase was seeded on the titanium alloy surface modified with or without the diamond-like carbon/casein phosphopeptide composite film.
RESULTS AND CONCLUSION: Compared with the titanium alloy without modification, the adhesion and proliferation of osteoblasts were significantly increased after modification by diamond-like carbon /casein phosphopeptide composite film (P < 0.05). The scanning electron microscope showed the osteoblast body on the surface of titanium alloy increased significantly, presenting with rough surface, fuzzy boundaries, and fully stretched after modification by diamond-like carbon/casein phosphopeptide composite film, but the osteoblasts on the surface of titanium alloy without modification displayed smooth surface, clear boundary, not fully stretched. It is indicated that the prepared diamond-like carbon/casein phosphopeptide composite film can significantly increase the biological activity of titanium alloy.

Key words: biomaterials, tissue engineering bone materials, tissue engineering oral materials, diamond-like carbon film, titanium alloy, casein phosphopeptides, osteoblasts, proliferation, adhesion, the National Natural Science Foundation of China, biomaterial photographs-containing paper

CLC Number:

R318

Bu Yin-zhong, Wang Lin, Ran Hai-qiong, Chen Jun-lin, Zhang Jun-yan, Liu Bin. Osteoblast proliferation on the surface of titanium alloy modified by diamond-like carbon/casein phosphopeptide composite film[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(8): 1367-1371.

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