Abstract:

BACKGROUND: The technology of nanoparticle carrier transferring gene can carry or enhance plant cell
sustainable stability, extend its release and maintain its activity and duration.
OBJECTIVE: To explore the effects of nanoparticle carrier with neurotrophic factor-3 on transfection of Schwann cells in facilitating and repairing sciatic nerve defects.
METHODS: The neurotrophic factor-3 genes were transfected to the Schwann cells of newborn Wistar rats with nanoparticle carrier. Eighty adult Wistar rats were selected to make the sciatic nerve defect model. Then four kinds of grafts were injected into the pipe bridge takeover of extracellular matrix gel-poly( lactide-co-glycolide): the blank control group was not injected with other substances, the cell group was injected with Schwann cells, the gene group was injected with neurotrophic factor-3 gene, and the experimental group was injected with neurotrophic factor-3 gene modified Schwann cells.
RESULTS AND CONCLUSION: The staining of sciatic nerve and muscle fiber cross-sectional area was better in the experimental group than in the cell group and gene group (P < 0.01), which was better in the cell and gene groups than the blank control group (P < 0.01); but, there was no significant difference between the cell group and gene group (P > 0.05). It indicated that neurotrophic factor-3 gene modified Schwann cells transplantation can reconstruct the microenvironment of nerve regeneration and promote repair of sciatic nerve defects.

Key words: biomaterials, nano-biomaterials, genetic engineering, nanoparticle carrier, neurotrophic factor-3, Schwann cells, nerve defects, provincial grants-supported paper, biomaterial photographs-containing paper