In vitro fabrication of tissue engineering osteochondral composites

doi:10.3969/j.issn.2095-4344.2013.08.005

Abstract

Abstract:

BACKGROUND: To design an integrated and biphasic scaffold that is cocultured with chondrocytes and bone marrow mesenchymal cells is beneficial to form good interface between bone and cartilage tissue.
OBJECTIVE: To construct a bilayered scaffold based on imitating natural osteochondral matrix that is cocultured with chondrocyte and bone marrow mesenchymal stem cells as seed cells so as to observe the chondrogenic and osteogenic capacity of the composite tissue.
METHODS: Gelatin-chondroitin sulfate-hyaluronic acid and gelatin-ceramic bone porous scaffolds were prepared to be cocultured with chondrocytes and bone marrow mesenchymal stem cells for osteogenic induction or non-osteogenic induction. MTT, glycosaminoglycan, alkaline phosphatase and hematoxylin-eosin staining were detected.
RESULTS AND CONCLUSION: MTT results showed the bone marrow mesenchymal stem cell proliferation and glycosaminoglycan contents had no statistical difference between the osteogenic induction and non-osteogenic induction groups. Alkaline phosphatase activity increased gradually in the non-osteogenic induction group, but increased rapidly in the osteogenic induction group that became stable at 14 days. No significant difference was found in the results of hematoxylin-eosin staining between the two groups. Osteochondral-like tissues were found in the two groups with non-degradable scaffolds. Cell-free regions were scattered within the scaffolds owing to the incomplete matrix formation and scaffold degradation. This study proved that these two kinds of seed cells cocultured with the bilayer scaffold in vitro can form tissue engineering osteochondral composite tissues.

Key words: biomaterials, tissue engineering bone materials, gelatin, chondroitin sulfate, hyaluronate, composite scaffolds, tissue engineering, osteochondral, bone marrow mesenchymal stem cells, alkaline phosphatase, chondrocytes, the National Natural Science Foundation of China, biomaterial photographs-containing paper

CLC Number:

R318

Deng Tian-zheng, Lü Jing, Yang Jie-fei, Ke Jie. In vitro fabrication of tissue engineering osteochondral composites[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(8): 1354-1361.

Figures/Tables 4

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