Distribution characteristics of epidermal stem cells after treatment with fractional CO2 laser in a model of skin photoaging

doi:10.3969/j.issn.2095-4344.2013.06.021

Abstract

Abstract:

BACKGROUND: There has been some progress in clinical application of fractional CO2 laser for treatment of facial photoaging. However, little is known about the change law of epidermal stem cells during wound healing process after treatment with fractional CO2 laser in a model of skin photoaging.
OBJECTIVE: To investigate the quantity and distribution of epidermal stem cells during wound healing process after treatment with fractional CO2 laser in a mouse model of skin photoaging.
METHODS: Ten Kunming mouse models of skin photoaging developed by exposure to ultraviolet radiation were treated with fractional CO2 laser (Deep FX). Wound healing was monitored and samples were harvested for determination of keratin 19 ad P63 expression by immunohistochemical staining before and 1, 3, 7 and 15 days after fractional CO2 laser treatment.
RESULTS AND CONCLUSION: The wounds almost healed at 15 days after treatment. Compared to before and day 1 treatment, Keratin 19 and P63 expression on day 3 were significantly increased, and keratin 19- and P63-positive cells were distributed in larger areas. Compared to days 1 and 3 of treatment, keratin 19- and P63-positive cells were significantly higher in quality and peaked on day 7 of treatment, and they were disorderly arranged in each epidermal layer. On day 15 of treatment, keratin 19- and P63-positive cells decreased in number and only accumulated in epidermal basal lamina and hair follicle eminence, and there was no significant difference in keratin 19- and P63-positive expression as compared with before and day 1 of treatment. These findings suggest that epidermal stem cells participate in the repair progress of skin photoaging in mice receiving fractional CO2 laser treatment and may play an important role in this process.

Key words: stem cells, stem cell culture and differentiation, epidermal stem cells, fractional CO2, keratin 19, P63, photoaging, stem cell photographs-containing paper

CLC Number:

R394.2

Tan Jun, Ding Wei, Li Gao-feng, Li Bo, Qin Xiao-dong. Distribution characteristics of epidermal stem cells after treatment with fractional CO2 laser in a model of skin photoaging[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(6): 1075-1080.

Figures/Tables 7

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