Biocompatibility of deer deproteinized cancellous bone

doi:10.3969/j.issn.2095-4344.2013.03.012

Abstract

Abstract:

BACKGROUND: Antigen-extracted xenogeneic cancellous bone as a kind of bone implant material has a natural porous structure, plasticity, and certain mechanical strength.
OBJECTIVE: To study the biocompatibility of the deer deproteinized cancellous bone.
METHODS: Pyrogen test and acute toxicity test: Fresh deer bone, deproteinized cancellous bone, andfree-dried deproteinized cancellous bone extracts were injected into the ear vein of rabbits and the abdominal cavity of mice. Hemolysis test: Rabbit mixed blood was added into the fresh deer bone, deproteinized cancellous bone, and free-dried deproteinized cancellous bone extracts, sodium carbonate (positive control), and normal saline (negative control). Coagulation test: Fresh deer bone, deproteinized cancellous bone, and free-dried deproteinized cancellous bone were placed into normal rabbit mixed blood. Long-term muscle test: Fresh deer bone, deproteinized cancellous bone, and free-dried deproteinized cancellous bone were implanted into the thigh muscle pouches of mice.
RESULTS AND CONCLUSION: Deer deproteinized cancellous bone and free-dried deproteinized cancellous bone had no pyrogen reaction, toxicity, hemolysis and hemopexis reactions, and did not result in rejection reaction after implantation to the thigh muscle pouches of mice. There was a mild abnormality for the fresh deer bone in pyrogen, coagulation, hemolysis and long-term muscle tests without death. These findings indicate that deer deproteinized cancellous bone and free-dried deproteinized cancellous bone have good biocompatibility.

Key words: biomaterials, material biocompatibility, fresh deer bone, deer deproteinized cancellous bone, deer free-dried deproteinized cancellous bone, biocompatibility, hemolysis tests, coagulation tests, acute toxicity tests, provincial grants-supported paper

CLC Number:

R318

Liu Hai-peng, Li Chun, Zhang Duo, Wu Dan-kai. Biocompatibility of deer deproteinized cancellous bone[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(3): 452-456.

Figures/Tables 2

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