Combination of Schwann cells, small intestinal submucosa and growth factor sustained-release microspheres for repair of peripheral nerve defects

doi:10.3969/j.issn.2095-4344.2013.03.013

Abstract

Abstract:

BACKGROUND: To combine Schwann cells (SCs) and small intestinal submucosa (SIS) with basic fibroblast growth factor (bFGF) is a feasible way to construct artificial nerve that possesses nerve activity and chemotaxis in vitro.
OBJECTIVE: To observe the recanalization of neural pathways after repairing peripheral defects with bFGF controlled release microspheres combined with SCs and SIS.
METHODS: Models of sciatic nerve defects were established in Sprague-Dawley rats, and then randomized into: experimental group repaired with bFGF controlled release microspheres combined with SCs and SIS, positive control group with SCs and SIS combined with free bFGF, negative control group with SCs and SIS, and blank control group with autologous nerve.
RESULTS AND CONCLUSION: The number of regenerated nerve fibers, number of DiI tracer-positive neurons, positive expression rate of S-100 and neurofilament protein, ultrastructure of regenerated myelin and axons, nerve conduction velocity and composite action potential in the experimental group were superior to those in the positive and negative control groups at 16 weeks after operation (P < 0.05). These findings indicate that bFGF controlled release microspheres combined with SCs and SIS can reconstruct the neural pathways following sciatic nerve injury.

Key words: biomaterials, biomaterials and drug-controlled release, basic fibroblast growth factor, small intestinal submucosa, Schwann cells, peripheral nerve defects, nerve conduction, sustained-release microspheres, provincial grants-supported paper, biomaterial photographs-containing paper

CLC Number:

R318

Zhang Kai-wei, Duan Hong, Xiang Zhou, Xiao Rui, Chen Jiu-yi. Combination of Schwann cells, small intestinal submucosa and growth factor sustained-release microspheres for repair of peripheral nerve defects[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(3): 457-464.

Figures/Tables 6

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