Protective effects of different doses of vascular endothelial growth factors on neural stem cells under hypoxic environment

doi:10.3969/j.issn.2095-4344.1709

Abstract

Abstract:

BACKGROUND: Vascular endothelial growth factor (VEGF) is a cytokine which can promote angiogenesis. Recently, some animal experiments and clinical studies have shown that VEGF expression is increased in acute ischemic stroke and spinal cord injury, which can inhibit cell apoptosis, but the mechanism is not clear.
OBJECTIVE: To intervene in hypoxic-induced neural stem cells with different doses of VEGF, and to explore the protective effects and mechanism of VEGF.
METHODS: Neural stem cells was obtained from the cerebral cortex of a fetal rat, which were randomly divided into blank control group, hypoxia group and low-, medium- and high-dose VEGF groups. The cells in the blank control group were cultured for 24 hours under 35% oxygen. The cells in the other four groups were cultured for 24 hours under 3% oxygen. In the low-, medium- and high-dose VEGF groups, 20, 40 and 80 μmol/L VEGF were supplemented into the medium, respectively. The proliferation and apoptosis of the cells were detected by cell counting kit-8 method and TUNEL method. The expression level of nerve cell apoptosis related proteins was detected by western blot.
RESULTS AND CONCLUSION: Compared with the blank control group, the proliferation of neural stem cells decreased, the number of apoptotic neural stem cells was increased significantly, the expression levels of Bcl-2 and nuclear factor κB in the cells were decreased, and the expression levels of Bax and TWIK were increased in the hypoxia group. VEGF at all the three doses could inhibit these phenomena, and 40 μmol/L VEGF showed the best efficacy. These results indicate that VEGF can affect cell apoptosis by interfering with the expression of apoptosis-related proteins, and then exert neuroprotective effects, especially at 40 μmol/L.

Key words: neural stem cells, hypoxic environment, vascular endothelial growth factor, apoptosis-related proteins, apoptosis, neuroprotection

CLC Number:

Zhou Jingxia, Chen Lin, Chen Bocan, Xing Huaijie, Yan Limin, Zeng Chaosheng, Wu Hairong, Huang Yusheng, Chen Jiegui . Protective effects of different doses of vascular endothelial growth factors on neural stem cells under hypoxic environment[J]. Chinese Journal of Tissue Engineering Research, 2019, 23(17): 2684-2689.

Figures/Tables 4

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