Bone repair using skeletal muscle stem cells combined with platelet-rich plasma

doi:10.3969/j.issn.2095-4344.1719

Abstract

Abstract:

BACKGROUND: Platelet-rich plasma contains a variety of active factors, which has certain advantages as a cytokine that promotes stem cell differentiation for bone tissue engineering.
OBJECTIVE: To investigate the effect of skeletal muscle stem cells combined with platelet-rich plasma to promote the repair of rabbit bone.
METHODS: (1) Rabbit platelet-rich plasma was prepared and skeletal muscle stem cells from rabbit soleus muscle were isolated. Twenty-eight New Zealand white rabbits were randomly divided into four groups (n=7/group), in which the proximal humeral bone defect model was prepared in each rabbit, followed by implantation of nothing (natural healing group), allogeneic skeletal muscle stem cells, autologous platelet-rich plasma or platelet-rich plasma combined with skeletal muscle stem cells, respectively. CT and histological examinations were performed to detect bone healing postoperatively.
RESULTS AND CONCLUSION: In the postoperative 4-week CT film, new bone formation was faster in the combination group than the other three groups (P < 0.05). No significant difference in the bone formation rate was observed in the skeletal muscle stem cell and platelet-rich plasma groups (P > 0.05), but the bone formation rate in these two groups was significantly better than that in the natural healing group (P < 0.05). (2) Histological score of the one defect specimens was highest in the combination group and lowest in the natural healing group, and there was no significant difference between the skeletal muscle stem cell and platelet-rich plasma groups. These results reveal that both platelet-rich plasma and skeletal muscle stem cells could promote bone repair, and their combination could achieve better outcomes in bone repair.

CLC Number:

Yin Nuo, Xue Feng, Xiao Haijun, Ding Liang, Yuan Junjie, Pan Mingmang, Yu Du, Ju Jinyong, Zhu Longzhang. Bone repair using skeletal muscle stem cells combined with platelet-rich plasma[J]. Chinese Journal of Tissue Engineering Research, 2019, 23(17): 2678-2683.

Figures/Tables 1

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