Biological characteristics of hypoxic preconditioned human amniotic mesenchymal stem cells

doi:10.3969/j.issn.2095-4344.3527

Abstract

Abstract: BACKGROUND: Stem cell transplantation has brought hope for many difficult diseases, but the survival number of transplanted cells is small and the secretion function is poor, so it is difficult to play a therapeutic effect, which hinders the clinical application of stem cell transplantation. Hypoxic preconditioning can effectively improve the biological characteristics of implanted cells, such as proliferation activity, anti-apoptotic ability and secretion function. Therefore, human amniotic mesenchymal stem cells pretreated with hypoxia may play a better therapeutic role in tissue damage repair.
OBJECTIVE: To study the effects of hypoxic preconditioning on the biological characteristics of human amniotic mesenchymal stem cells.
METHODS: The mechanical method and enzymatic digestion were used to separate human amniotic mesenchymal stem cells, and then cultured it up to the third generation. After having hypoxic pretreatment (3% O2) for 48 hours, the nomoxic (21% O2) control group was set. The proliferation activity and DNA synthesis rate of human amniotic mesenchymal stem cells in the two groups were detected by CCK-8 and Edu proliferation experiments. The two groups of cells were cultured by serum-free medium for 24 hours. Apoptosis was detected using Annexin V-FITC/PI assay. The supernatant was collected. The levels of angiogenic factors secreted in the supernatant were detected by using the Proteome Profiler.
RESULTS AND CONCLUSION: Hypoxic preconditioning does not affect the characteristics of human amniotic mesenchymal stem cells, but can improve the proliferation activity of human amniotic mesenchymal stem cells; in serum-free culture conditions, hypoxic preconditioning can enhance the anti-apoptotic effect of human amniotic mesenchymal stem cells, and secrete more angiogenic factors.

Key words: stem cells, human amniotic mesenchymal stem cells, hypoxic, preconditioning, biological characteristics, proliferation, apoptosis, angiogenesis

CLC Number:

Dai Min, Wang Shuai, Zhang Nini, Huang Guilin, Yu Limei, Hu Xiaohua, Yi Jie, Yao Li, Zhang Ligang. Biological characteristics of hypoxic preconditioned human amniotic mesenchymal stem cells[J]. Chinese Journal of Tissue Engineering Research, 2021, 25(19): 3004-3008.

Figures/Tables 6

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