Chinese Journal of Tissue Engineering Research ›› 2019, Vol. 23 ›› Issue (30): 4841-4847.doi: 10.3969/j.issn.2095-4344.1409

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Preparation and biocompatibility evaluation of modified thermosensitive xyloglucan hydrogels

Yan Zhiwen1, Li Shuofeng1, Li Ao1, Hu Ziqi1, Ma Litao1, Zhang Ershuai2, Li Jingwu3, Yao Fanglian2, Che Pengcheng1, Sun Hong1 
  

  1. 1Key Laboratory for Chronic Diseases of Hebei Province, School of Basic Medical Sciences, North China University of Science and Technology, Tangshan 063210, Hebei Province, China; 2Department of Polymer Science and Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300350, China; 3Department of Gastrointestinal Surgery, Tangshan People’s Hospital, Tangshan 063001, Hebei Province, China
  • Received:2019-05-10 Online:2019-10-28 Published:2019-10-28
  • Contact: Sun Hong, MD, Professor, Chief physician, Master’s supervisor, Key Laboratory for Chronic Diseases of Hebei Province, School of Basic Medical Sciences, North China University of Science and Technology, Tangshan 063210, Hebei Province, China
  • About author:Yan Zhiwen, Master candidate, Key Laboratory for Chronic Diseases of Hebei Province, School of Basic Medical Sciences, North China University of Science and Technology, Tangshan 063210, Hebei Province, China
  • Supported by:

    the Natural Science Foundation of Hebei Province, No. H2017209005 (to SH); Tangshan Science and Technology Innovation Team Training Program Project, No.18130211B (to SH); the National College Students Innovation and Entrepreneurship Training Program, No. 201810081005 (to LSF, LA); Graduate Student Innovation Project of North China University of Science and Technology, No. 2018S26 (YZW); College Students Innovation and Entrepreneurship Training Program of North China University of Science and Technology, No. X2018009 (to LSF, LA)

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Abstract:

BACKGROUND: Injectable thermosensitive hydrogels have reversible phase transition with the change of ambient temperature and can be used in combination with minimally invasive therapy. This has attracted extensive attention in the field of postoperative adhesion prevention. However, the current injectable thermosensitive hydrogels for the prevention of postoperative adhesion have the problems of poor biosafety and anti-adhesion effects.
OBJECTIVE: To prepare a kind of hydrogel with thermosensitive and injectable characters with the modified xyloglucan and to evaluate its biocompatibility.
METHODS: Partial galactose of the side chain of xyloglucan was removed using mild enzymatic hydrolysis to obtain modified xyloglucan with reversible “sol-gel” transformation. The toxicity of modified xyloglucan hydrogel leaching liquor to human hepatoma cell line HepG2 and human lung adenocarcinoma cell line A549 was determined by acridine orange and propidium iodide staining and MTT assay. After 24 hours of culture, cell morphology was observed. Modified xyloglucan hydrogel leaching liquor, 0.1 and 1 g/L modified xyloglucan solution was added to the anticoagulated rabbit blood. The hemolysis rate was detected 1 hour later. The modified xyloglucan solution was subcutaneously injected into the back of the C57 mice (provided by Beijing Experimental Animal Center of the Chinese Academy of Military Medical Sciences, China) to track the in vivo degradation behavior of the material and evaluate the inflammatory response of the implant site and surrounding tissues. Animal experiments were approved by the Animal Ethics Committee, North China University of Science and Technology, China (approval No. SYXK (Ji) 2015-0038).
RESULTS AND CONCLUSION: The acridine orange and propidium iodide staining and MTT assay revealed that the proliferation activity of HepG2 cells and A549 cells in different concentrations of modified xyloglucan hydrogel leaching liquor was about 100%. A549 cells embedded in modified xyloglucan hydrogel still had good viability. The hemolysis rate of the modified xyloglucan leaching liquor and 0.1, 1 g/L modified xyloglucan solution was both below 2%. As the injection time prolonged, the modified xyloglucan hydrogel in the animal body gradually became smaller, and the degradation was complete at about 6 weeks. Inflammatory reaction could be caused in the early stage of injection, but with the prolongation of time and degradation of the material, the inflammatory reaction gradually reduced and disappeared, and no obvious tissue lesions were observed in the injection site. These results suggest that the modified xyloglucan hydrogel is thermosensitive and has good cell compatibility, blood compatibility, and biodegradability.

Key words: modified xyloglucan, xyloglucan, injectable thermosensitive hydrogel, biocompatibility, postoperative adhesion, blood compatibility, biomaterial, human hepatoma cell line, human lung adenocarcinoma cell line

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