Preparation and properties of poly3-hydroxybutyrate 4-hydroxybutyrate/polyethylene glycol/graphene oxide tissue-engineered scaffolds

doi:10.3969/j.issn.2095-4344.3177

Chinese Journal of Tissue Engineering Research ›› 2021, Vol. 25 ›› Issue (22): 3466-3472.doi: 10.3969/j.issn.2095-4344.3177

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Preparation and properties of poly3-hydroxybutyrate 4-hydroxybutyrate/polyethylene glycol/graphene oxide tissue-engineered scaffolds

Liu Jun1, 2, 3, Yang Long1, Wang Weiyu1, 2, 3, Zhou Yuhu1, 2, 3, Wu Ying3, 4, Lu Tao3, Shu Liping2, 3, Ma Minxian4, Ye Chuan1, 2, 3

1Department of Orthopedics, Affiliated Hospital of Guizhou Medical University, Guiyang 550004, Guizhou Province, China; 2National-Guizhou Joint Engineering Laboratory of Cell Engineering and Biomedicine Technique, 3Research Center of Tissue Engineering and Stem Cell Technique, Guizhou Medical University, Guiyang 550004, Guizhou Province, China; 4Department of Prosthodontics, Affiliated Stomatology Hospital of Guizhou Medical University, Guiyang 550004, Guizhou Province, China

Received:2020-04-18 Revised:2020-04-23 Accepted:2020-06-17 Online:2021-08-08 Published:2021-01-19
Contact: Ye Chuan, Chief physician, Department of Orthopedics, Affiliated Hospital of Guizhou Medical University, Guiyang 550004, Guizhou Province, China; National-Guizhou Joint Engineering Laboratory of Cell Engineering and Biomedicine Technique, and Research Center of Tissue Engineering and Stem Cell Technique, Guizhou Medical University, Guiyang 550004, Guizhou Province, China
About author:Liu Jun, Master candidate, Physician, Department of Orthopedics, Affiliated Hospital of Guizhou Medical University, Guiyang 550004, Guizhou Province, China; National-Guizhou Joint Engineering Laboratory of Cell Engineering and Biomedicine Technique, and Research Center of Tissue Engineering and Stem Cell Technique, Guizhou Medical University, Guiyang 550004, Guizhou Province, China
Supported by:
the National Natural Science Foundation of China, No. 8196046, (to YC); a grant from Department of Science and Technology of Guizhou Province, No. [2020] 6013, No. [2020]4Y137 (to YC)

Abstract

Abstract: BACKGROUND: The application of poly3-hydroxybutyrate 4-hydroxybutyrate (P34HB) scaffolds in tissue engineering has been limited due to lacking hydrophilicity.
OBJECTIVE: To explore the physicochemical properties and biocompatibility of P34HB as well as polyethylene glycol (PEG) co-solvent mixed graphene oxide (GO) electroplating scaffolds.
METHODS: The scaffolds of P34HB, P34HB/PEG and P34HB/PEG/GO had been prepared by electrospinning technology. The three-dimensional structure of the scaffold had been observed by scanning electron microscopy. The contact angle of water droplets on the scaffold had been measured by optical measuring instrument. The tensile stress, strain and elastic modulus of electrospinning frame were analyzed by mechanical tester. The three kinds of scaffolds had been co-cultured with rat bone marrow mesenchymal stem cells respectively. Cell adhesion had been detected by MTT assay. Cell proliferation was detected by Alamar blue assay. Cell survival rate had been observed by Live/Dead fluorescence staining. Cell morphology had been observed by scanning electron microscopy.
RESULTS AND CONCLUSION: (1) Scanning electron microscopy had showed random distribution of scaffold fibers in the three groups, and the fiber diameters of P34HB, P34HB/PEG, and P34HB/PEG/GO groups gradually decreased. (2) The contact angles of P34HB/PEG and P34HB/PEG/GO scaffolds were smaller than that of the P34HB scaffold (P < 0.01). (3) The strain rates of P34HB/PEG and P34HB/PEG/GO scaffolds were lower than that of P34HB scaffold (P < 0.05 or
P < 0.01), and the elastic modulus of P34HB/PEG/GO scaffolds was higher than that of P34HB/PEG scaffold (P < 0.05). There was no significant difference in the tensile strength among the three groups (P > 0.05). (4) At 1, 3, and 6 hours of co-culture, the number of cell adhesion in the P34HB/PEG/GO group was higher than that in the P34HB and P34HB/PEG groups (P < 0.01), and the number of cell adhesion in the P34HB/PEG group was higher than that in the P34HB group
(P < 0.01). (5) At 4 and 7 days of co-culture, the proliferation of cells in the P34HB/PEG/GO group was faster than that in the P34HB group and P34HB/PEG group (P < 0.05 or P < 0.01). (6) After 7 days of co-culture, scanning electron microscopy showed that the surface cell volume of P34HB/PEG/GO scaffold was largest and best spread, and the cell survival rate of Live/Dead fluorescence staining group was highest. (7) The results have showed that P34HB/PEG/GO electrospinning scaffold had certain mechanical properties and good hydrophilicity, and could effectively promote cell proliferation and adhesion.

Key words: bone, material, graphene oxide, polyethylene glycol, tissue-engineered, scaffold, electrospun, biomaterial

CLC Number:

Liu Jun, Yang Long, Wang Weiyu, Zhou Yuhu, Wu Ying, Lu Tao, Shu Liping, Ma Minxian, Ye Chuan. Preparation and properties of poly3-hydroxybutyrate 4-hydroxybutyrate/polyethylene glycol/graphene oxide tissue-engineered scaffolds[J]. Chinese Journal of Tissue Engineering Research, 2021, 25(22): 3466-3472.

Figures/Tables 9

References

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Preparation and properties of poly3-hydroxybutyrate 4-hydroxybutyrate/polyethylene glycol/graphene oxide tissue-engineered scaffolds

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