Chinese Journal of Tissue Engineering Research ›› 2019, Vol. 23 ›› Issue (26): 4230-4236.doi: 10.3969/j.issn.2095-4344.1365

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Preparation of sodium alginate-hydroxyapatite-polyethylene glycol composite hydrogel scaffold and its biocompatibility

Li Xuanze1, 2, 3, Shu Liping3,4, Chen Jiao4, 5, Sun Yu4, Zou Qiang2, Wang Weiyu4, Liu Jun4, Yang Long2, Ma Minxian4, 5, Ye Chuan2, 3, 4
  


  • Received:2019-04-22 Online:2019-09-18 Published:2021-04-29
  • Contact: Ye Chuan, Chief physician, Department of Orthopedics, Affiliated Hospital of Guizhou Medical University, Guiyang 550004, Guizhou Province, China; National-Guizhou Joint Engineering Laboratory of Cell Engineering and Biomedicine Technique, Guiyang 550004, Guizhou Province, China; Research Center of Tissue Engineering and Stem Cell Technique, Guizhou Medical Univesity, Guiyang 550004, Guizhou Province, China
  • About author:Li Xuanze, Master candidate, Physician, Guizhou Medical University, Guiyang 550004, Guizhou Province, China; Department of Orthopedics, Affiliated Hospital of Guizhou Medical University, Guiyang 550004, Guizhou Province, China; National-Guizhou Joint Engineering Laboratory of Cell Engineering and Biomedicine Technique, Guiyang 550004, Guizhou Province, China
  • Supported by:

    the National Natural Science Foundation of China, No. 81360232 (to YC); Guiyang Science and Technology Bureau Innovation Team Project, No. [2017]5-17 (to YC); an Open Project of Key Laboratory of Adult Stem Cell Transplantation of Chinese Academy of Medical Sciences, No. 2018YB04 (to YL); a Project of Guizhou Provincial Department of Education, No. qianjiaohe-KY[2017]161 (to YL)

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Abstract:

BACKGROUND: Polyethylene glycol, as a medical polymer copolymer, is widely used in sustained-release of drugs. If it is prepared into composite hydrogel, it can be used as a scaffold material with certain mechanical properties and good biocompatibility.
OBJECTIVE: To investigate the physical and chemical properties and biocompatibility of sodium alginate-hydroxyapatite-polyethylene glycol (SA-HA-PEG) composite hydrogel scaffold material.
METHODS: Bivalent cationic solution was added to the ultrasound treated hydrogel for crosslinking. Four groups of scaffold were obtained: SA-HA scaffold (group A), SA-HA-3%PEG (group B), SA-HA-5%PEG (group C), SA-HA-8%PEG (group D). The mass concentration of PEG in the later three groups was 30, 50, and 80 g/L. The compressive stress and elastic modulus of the hydrogel scaffold were analyzed using a dynamic mechanical analyzer. The scaffold was placed in lysozyme solution to determine its degradation rate in vitro. After vacuum freeze-drying, the three-dimensional structure of the hydrogel scaffold was observed by scanning electron microscopy. Four groups of hydrogel scaffold were cross-linked with bone marrow mesenchymal stem cells. Cell proliferation was detected using CCK8 assay at 1, 3, 5 and 7 days of culture. After 7 days of culture, cell viability was detected using Live/Dead fluorescence staining.
RESULTS AND CONCLUSION: The compressive stress and elastic modulus in the groups B, C and D were significantly higher than those in the group A (P < 0.05). The compressive stress and elastic modulus in the groups C and D were significantly higher than those in the group B (P < 0.05). There were no significant differences in compressive stress and elastic modulus between groups C and D (P > 0.05). The degradation rate of hydrogel scaffold in the groups C and D were significantly lower than that in the groups A and B (P < 0.05). The degradation rate of hydrogel scaffold in the group B was significantly lower than that in the group A (P < 0.05). There was no significant difference in the degradation rate of hydrogel scaffold between groups C and D (P > 0.05). Scanning electron microscopy showed that hydrogel scaffold in the group C had the structure with high porosity and consistent pore size; hydrogel scaffold in the groups A, B and D had the structure with low porosity and inconsistent pore size. After 3-7 days of culture, cells in the groups B, C and D proliferated more rapidly than those in the group A (P < 0.05). There was no significant difference in cell proliferation rate between groups B, C and D (P < 0.05). After 7 days of culture, cell viability in the groups B, C and D was significantly higher than that in the group A (P < 0.05), and cell viability in the group C was significantly higher than that in the groups B and D (P < 0.05). These results suggest that SA-HA-PEG composite hydrogel scaffold exhibit good mechanical property and encouraging biocompatibility.  

Key words: biomaterial, SA-HA-PEG, sodium alginate, hydroxyapatite, polyethylene glycol, hydrogel, tissue-engineered scaffold, composite hydrogel scaffold

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