Vascular endothelial growth factor 165/bone morphogenetic protein improves osteoblast injury under hypoxic and reoxygenated conditions

doi:10.12307/2024.809

Abstract

Abstract: BACKGROUND: It has been found that vascular endothelial growth factor 165 and bone morphogenetic proteins interact with each other during hypoxia-reoxygenation and are involved in the repair process of osteoblast injury by regulating the activation of intracellular signaling pathways.
OBJECTIVE: To further investigate the relationship between vascular endothelial growth factor 165/bone morphogenetic protein and hypoxic-reoxygenated osteoblast injury.
METHODS: Osteoblasts were selected and the hypoxic-reoxygenated injury model was established. Vascular endothelial growth factor 165 and bone morphogenetic protein expressions at mRNA and protein levels were detected by real-time PCR and western blot before and after modeling. After modeling, osteoblasts were given different concentrations of vascular endothelial growth factor 165 and bone morphogenetic protein 2 (10, 20, 40 ng/mL). Cell proliferation was detected by cell counting kit-8 method and apoptosis was detected by DAPI at 12, 24, 36, 48, and 72 hours after treatment.
RESULTS AND CONCLUSION: Compared with before modeling, the mRNA and protein expressions of vascular endothelial growth factor 165 and bone morphogenetic protein 2 in osteoblasts after modeling were significantly decreased (P < 0.05). The proliferation rate of osteoblasts was significantly increased with the increase of vascular endothelial growth factor 165 concentration (P < 0.05), while the apoptosis rate of osteoblasts decreased significantly with the increase of vascular endothelial growth factor 165 concentration (P < 0.05). The proliferation rate of osteoblast was significantly increased with the increase of bone morphogenetic protein 2 concentration (P < 0.05), while the apoptosis rate of osteoblast decreased significantly with the increase of bone morphogenetic protein 2 concentration (P < 0.05). To conclude, vascular endothelial growth factor 165 and bone morphogenetic protein are lowly expressed in hypoxic-reoxygenated osteoblast injury, and treatment with vascular endothelial growth factor 165 and bone morphogenetic protein can reduce the injury of hypoxic-reoxygenated osteoblast in a concentration-dependent manner, suggesting that vascular endothelial growth factor 165 and bone morphogenetic protein have a significant protective effect against the injury of hypoxic-reoxygenated osteoblasts.

Key words: vascular endothelial growth factor 165, bone morphogenetic protein 2, hypoxia-reoxygenation, osteoblast, cell apoptosis

CLC Number:

Zhao Yiting, Zhang Yuxiang, Ma Jie, He Xuejiao. Vascular endothelial growth factor 165/bone morphogenetic protein improves osteoblast injury under hypoxic and reoxygenated conditions[J]. Chinese Journal of Tissue Engineering Research, 2024, 28(35): 5669-5674.

Figures/Tables 8

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