Caveolin-1 mediated fluid shear stress regulates proliferation and apoptosis of MC3T3-E1 osteoblasts

doi:10.12307/2024.840

Abstract

Abstract: BACKGROUND: Fluid shear stress plays an important role in osteoblast proliferation and apoptosis. However, whether Caveolin-1 is involved in the process of fluid shear stress-induced proliferation and apoptosis in osteoblasts is unknown.
OBJECTIVE: To explore the role of Caveolin-1 in fluid shear stress-regulated osteoblast proliferation and apoptosis.
METHODS: The MC3T3-E1 osteoblasts in good growth status were selected and loaded with fluid shear stress at an intensity of 1.2 Pa for different times (0, 30, 60, 90 minutes). The expression of Caveolin-1 protein was observed and conditions with a time of 60 minutes were screened for the experiment. MC3T3-E1 cells were divided into control group, fluid shear stress group, fluid shear stress+pcDNA 3.1 group (control), fluid shear stress+pcDNA Cav-1 group (plasmid overexpression), and intervened with fluid shear stress and overexpression of Cav-1, respectively. The expression of molecules related to proliferation and apoptosis in MC3T3-E1 cells was detected by qRT-PCR and western blot. In addition, the proliferative activity of MC3T3-E1 cells was detected by cell counting kit-8 and EdU assay; and cell apoptosis was detected by Hoechst 33258 and flow cytometry.
RESULTS AND CONCLUSION: The expression of Caveolin-1 in MC3T3-E1 cells was significantly down-regulated after loading fluid shear stress, and the expression level was lowest after 60 minutes. Overexpression of Caveolin-1 attenuated the proliferation-promoting and apoptosis-suppressing effects of fluid shear stress in MC3T3-E1 cells. In conclusion, Caveolin-1 has a vital role in fluid shear stress-regulated osteoblast proliferation and apoptosis, which may offer a potential therapeutic strategy for osteoporosis.

Key words: osteoporosis, fluid shear stress, Caveolin-1, osteoblast, proliferation, apoptosis

CLC Number:

Yi Zhi, Zhan Hongwei, Wang Yaobin, Liang Xiaoyuan, Niu Yongkang, Xiang Dejian, Geng Bin, Xia Yayi. Caveolin-1 mediated fluid shear stress regulates proliferation and apoptosis of MC3T3-E1 osteoblasts[J]. Chinese Journal of Tissue Engineering Research, 2024, 28(34): 5440-5445.

Figures/Tables 6

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