Physicochemical properties and biocompatibility of graphene oxide-hydroxyapatite composite coating materials

doi:10.12307/2024.259

Abstract

Abstract: BACKGROUND: Medical titanium and titanium alloy have achieved good therapeutic effects in clinical applications, but there are still some phenomena such as peri-implant inflammation, loosening and shedding.
OBJECTIVE: To explore the physicochemical properties of graphene oxide coating materials and their effects on bone marrow mesenchymal stem cells.
METHODS: (1) Hydroxyapatite coating and graphene oxide/hydroxyapatite composite coating were prepared on a titanium surface by electrochemical deposition. The surface morphology, phase structure, functional groups, elemental composition and surface hydrophilicity of the coating were analyzed by scanning electron microscopy, X-ray energy dispersion spectroscopy, X-ray photoelectron spectroscopy, X-ray diffraction, Raman spectroscopy, and contact angle measurement instrument. (2) Mouse bone marrow mesenchymal stem cells were isolated and cultured and inoculated on pure titanium, hydroxyapatite coating and graphene oxide/hydroxyapatite composite coating, respectively. CCK-8 assay and scanning electron microscopy were used to evaluate the proliferation, morphology and growth status of the coated cells.
RESULTS AND CONCLUSION: (1) Scanning electron microscopy and X-ray energy dispersion spectra showed that the surface of graphene oxide/hydroxyapatite composite coating was more flat, compact and uniform than that of hydroxyapatite coating and pure titanium. X-ray photoelectron spectroscopy, X-ray diffraction and Raman spectroscopy showed that hydroxyapatite coating and graphene oxide/hydroxyapatite composite coating were successfully prepared on the surface of the titanium sheet. The hydrophilicity of graphene oxide/hydroxyapatite composite coating was better than that of hydroxyapatite coating and pure titanium. (2) CCK-8 assay showed that the number of bone marrow mesenchymal stem cells on the surface of the three groups increased with the extension of co-culture time. On days 1, 4, and 7, the proliferative absorbance of the cells on the graphene oxide/hydroxyapatite composite coating was higher than that on hydroxyapatite coating and pure titanium (P < 0.000 1). Scanning electron microscopy after 4 days of co-culture showed that bone marrow mesenchymal stem cells on the surface of the three groups of materials were fusiform. The cells on the surface of graphene oxide/hydroxyapatite composite coating showed good tensile properties and were polygonal. Multiple antennae could be seen attached to the coating surface at the edge of the cells, and the elongation of the filamentous foot on the surface was more than that on pure titanium and hydroxyapatite coatings. (3) The results show that graphene oxide/hydroxyapatite composite coating material is a kind of coating material with excellent physicochemical and biological properties.

Key words: graphene oxide, hydroxyapatite, electrochemical deposition, bone marrow mesenchymal stem cell, biocompatibility

CLC Number:

Lyu Shangyi, He Huiyu, Wufanbieke · Baheti, Yang Quan, Ma Lisha, Han Xiangzhen. Physicochemical properties and biocompatibility of graphene oxide-hydroxyapatite composite coating materials[J]. Chinese Journal of Tissue Engineering Research, 2024, 28(10): 1477-1483.

Figures/Tables 13

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