中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (19): 3540-3544.doi: 10.3969/j.issn.1673-8225.2012.19.025

• 骨髓干细胞 bone marrow stem cells • 上一篇    下一篇

骨髓间充质干细胞移植治疗急性一氧化碳中毒迟发性脑病大鼠*☆

邢红霞1，2，殷  闯3，刘  胜4，朱元凯2，史莉瑾2，田小军2，张博爱1，王玉梅2   

  1. 1郑州大学第一附属医院神经内科，河南省郑州市 450001；新乡医学院第一附属医院，2神经内一科，  4普外二科，河南省卫辉市453100；3新乡医学院第三附属医院神经内科，河南省新乡市453003
     
  • 收稿日期:2011-09-10 修回日期:2011-10-03 出版日期:2012-05-06 发布日期:2012-05-06
  • 通讯作者: ：张博爱，教授，主任医师，郑州大学第一附属医院神经内科，河南省郑州市450001 zhangboaidoctor@163.com 并列通讯作者：王玉梅，主任医师，新乡医学院第一附属医院神经内一科，河南省卫辉市 453100 yumei2628@163.com
  • 作者简介:邢红霞☆，女，1972年生，河南省扶沟县人，汉族，郑州大学在读博士，副主任医师，副教授，主要从事神经变性疾病研究。 xhxwh02@163.com xhxwh02@gmail.com
  • 基金资助:

    2007年河南省科技厅立项项目(072102310077)。

Bone marrow stem cells transplantation for treatment of delayed encephalopathy after acute carbon monoxide poisoning in rats

Xing Hong-xia1, 2, Yin Chuang3, Liu Sheng4, Zhu Yuan-kai2, Shi Li-jin2, Tian Xiao-jun2, Zhang Bo-ai1, Wang Yu-mei2   

  1. 1Department of Neurology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou  450001, Henan Province, China; 2First Department of Neurology, 4Second Department of General Surgery, the First Affiliated Hospital of Xinxiang Medical College, Weihui  453100, Henan Province, China; 3Department of Neurology, the Third Affiliated Hospital of Xinxiang Medical College, XinXiang  453003, Henan Province, China
  • Received:2011-09-10 Revised:2011-10-03 Online:2012-05-06 Published:2012-05-06
  • Contact: Zhang Bo-ai, Professor, Chief physician, Department of Neurology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450001, Henan Province, China zhangboaidoctor@163.com Wang Yu-mei, Chief physician, No.1 Department of Neurology, the First Affiliated Hospital of Xinxiang Medical College, Weihui 453100, Henan Province, China yumei2628@163.com
  • About author:Xing Hong-xia☆, Studying for doctorate, Associate chief physician, Associate professor, Department of Neurology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450001, Henan Province, China; First Department of Neurology, the First Affiliated Hospital of Xinxiang Medical College, Weihui 453100, Henan Province, China xhxwh02@163.com; xhxwh02@gmail.com
  • Supported by:

    Established Program in 2007 of Henan Science and Technology Bureau, No. 072102310077*

PDF

312

被引次数

5

摘要：

背景：国内多采用药物治疗和高压氧治疗一氧化碳中毒迟发性脑病，但存在疗程长，见效慢，花费高等问题。
目的：观察骨髓间充质干细胞移植治疗一氧化碳中毒迟发性脑病前后神经元及细胞凋亡的变化。
方法：将SD大鼠抽签随机分为假手术组、对照组与干细胞移植组。制作一氧化碳中毒迟发性脑病模型后，干细胞移植组在造模后第0，3，6，12，24，72小时及1周时脑内注射同种异体骨髓间充质干细胞，对照组不进行细胞移植；假手术组扎闭颈外动脉，用PBS代替细胞悬液。
结果与结论：造模后3，6，12，24 h干细胞移植组脑内神经元核心抗原表达明显高于对照组与假手术组(P < 0.01)，干细胞移植各组脑内神经元核心抗原表达无明显差异(P > 0.05)。造模后0，3，6 h干细胞移植组脑内细胞凋亡平均吸光度值明显低于对照组与假手术组及其他干细胞移植组(P < 0.05)，且移植后第1周明显低于第2，3，4周(P < 0.05)。提示在一氧化碳中毒迟发性脑病大鼠体内移植间充质干细胞可以增加成熟神经元数量，其机制与减少细胞凋亡有关；移植的最佳时机为发病后0~24 h之间，细胞移植后的积极作用在第1周最明显。
关键词：一氧化碳中毒迟发性脑病；骨髓间充质干细胞；神经元；凋亡；干细胞移植
doi:10.3969/j.issn.1673-8225.2012.19.025

关键词: 一氧化碳中毒迟发性脑病, 骨髓间充质干细胞, 神经元, 凋亡, 干细胞移植

Abstract:

BACKGROUND: The therapy of delayed encephalopathy after acute carbon monoxide poisoning (DEACMP) in our country mainly depends on drug or hyperbaric oxygen. But the treatment has the characters of longer course, lower effect and higher cost.
OBJECTIVE: To observe the changes of mature neurons and cell apoptosis before and after bone marrow mesenchymal stem cells (BMSCs) transplantation for treatment of DEACMP.
METHODS: SD rats were randomly divided into three groups: sham operation group, control group and transplantation group. The rat model with DEACMP was established and intracerebral injection of allogeneic BMSCs was performed in the transplantation group at 0, 3, 5, 12, 24, 72 hours and 1 week after modeling, and cell transplantation was not performed in the control group. In the sham operation group, the external carotid artery was tied off and the PBS was injected instead of cell suspension. 
RESULTS AND CONCLUSION: The expression of neuron-core protein (NeuN) in rat brain in the transplantation group at 3, 6,12 and 24 hours were significantly higher than that in the control group and sham operation group (P < 0.01). There was no significant difference in NeuN expression at 3, 6, 12 and 24 hours (P > 0.05). The average absorbance value of apoptosis in the transplantation group across 0-hour, 3-hour and 6-hour three time points was significantly lower than that in the sham operation group and other transplantation groups (P < 0.05). The average absorbance value of apoptosis in 1 week group was significantly lower than that in 2-week, 3-week and 4-week groups (P < 0.05). Transplantating BMSCs into the DEACMP rat models through the carotid artery could increase the quantity of mature neurons, and the underlying mechanism has relationship with apoptosis reduction. The best time for transplantation was between the 0 hour and the 24 hours after falling ill and the positive effect was significant at 1 week after transplantation.

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