中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (16): 2913-2916.doi: 10.3969/j.issn.1673-8225.2012.16.015

• 药物控释材料 drug delivery materials • 上一篇    下一篇

一种新型热敏聚乳酸微球的制备与表征★

彭  坤1, 2，王远亮1，甘哓玲2，王易振2，阮长顺1，张静文2，王  抒2，孙双凌2，陈俊意2   

  1. 1重庆大学生物工程学院生物材料与仿生工程中心，重庆市  400030；2重庆医药高等专科学校医学技术系，重庆市  401331
  • 收稿日期:2011-10-15 修回日期:2012-03-09 出版日期:2012-04-15 发布日期:2012-04-15
  • 通讯作者: 王远亮，教授，重庆大学生物工程学院生物材料与仿生工程中心，重庆市 400030 wyl@cqu.edu.cn
  • 作者简介:彭坤★，男，1982年生，重庆市人，汉族，2008年重庆大学毕业，硕士，讲师，主要从事生物材料与组织工程的研究。 pk3001@ 163.com

Preparation and characterization of a new-type thermal-sensitive polylactic acid microsphere

Peng Kun1,2, Wang Yuan-liang1, Gan Xiao-ling2, Wang Yi-zhen2, Ruan Chang-shun1, Zhang Jing-wen2, Wang Shu2, Sun Shuang-ling2, Chen Jun-yi2   

  1. 1Research Centre of Biomaterial and Bionic Engineering, Bioengineering College of Chongqing University, Chongqing  400030, China; 2Department of Medical Technology, Chongqing Medical and Pharmaceutical College, Chongqing  401331, China
  • Received:2011-10-15 Revised:2012-03-09 Online:2012-04-15 Published:2012-04-15
  • Contact: author: Wang Yuan-liang, Professor, Department of Research Centre of Biomaterial and Bionic Engineering, Bioengineering College of Chongqing University, Chongqing 400030, China wyl@cqu.edu.cn
  • About author:Peng Kun★, Master, Lecturer, Research Centre of Biomaterial and Bionic Engineering, Bioengineering College of Chongqing University, Chongqing 400030, China; Department of Medical Technology, Chongqing Medical and Pharmaceutical College, Chongqing 401331, China pk3001@163.com

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摘要：

背景：目前，基于聚乳酸的智能型微球是药物可控释放研究领域的热点。
目的：制备一种温度响应可控、生物可降解的微球载体。
方法：以甲基丙烯酸-2-羟乙酯作为丙交酯开环的引发剂，采用辛酸亚锡作为催化剂，并调节乳酸与甲基丙烯酸乙酯的比例，得到了不同分子质量的带双键聚乳酸(PDLLA-EMA)。以甲苯为溶剂，采用溶液合成法，自由基反应引发含有双键的聚乳酸以及N，N-异丙基丙烯酰胺共聚，成功制得了可降解温敏型共聚物(PNIPA-g-PDLLA-EMA)。采用复乳法，将PNIPA-g-PDLLA-EMA制成了微球。
结果与结论：实验制得PNIPA-g-PDLLA-EMA材料的热响应温度范围为35~42 ℃，基于该材料制得的微球粒径范围为(13.70±0.70)~(28.90±0.50) μm，粒径变化率为(138.7±4.20)%~(170.0±10.00)%，与同类材料相比，该微球已具备了应用于生物医学的潜在条件。
关键词：智能型微球；可生物降解；聚乳酸；制备；载体
缩略语注释：PNIPAm：Poly N-isopropylacrylamide，聚(N-异丙基丙烯酰胺)
doi:10.3969/j.issn.1673-8225.2012.16.015

关键词: 智能型微球, 可生物降解, 聚乳酸, 制备, 载体

Abstract:

BACKGROUND: At present, the research of controlled drug release has been focused on intelligent microspheres based on polylactic acid.
OBJECTIVE: To prepare a temperature-controllable and biodegradable microsphere carrier.
METHODS: First, 2-hydroxyethyl methacrylate was used as evocating agent for ring-opening polymerization of lactide, and stannous octoate used as catalyst. Through regulating the proportion of lactide and ethyl methacrylate, a double-bound polylactic acid ethyl methacrylate (PDLLA-EMA) of different molecular masses was obtained. After that, methylbenzene was used as solvent, and the copolymerizations of PDLLA-EMA with double bound and poly N-isopropylacrylamide (PNIPAm) were initiated by free radical reaction with a solution synthesis method, and then the biodegradable and temperature-sensitive     PNIPA-g-PDLLA-EMA was prepared successfully. At last, the thermal-sensitive polylactic acid microspheres were prepared by a double emulsion method.
RESULTS AND CONCLUSION: The thermal sensitive temperature of PNIPA-g-PDLLA-EMA prepared was in a range of   35-42 ℃. The range of microsphere particle size was (13.70±0.70) to (28.90±0.50) μm, and the change rate of particle size was (138.7±4.20)% to (170.0±10.00)%. Compared with other same type materials, this kind of microsphere has potentialities in biomedicine.

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