中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (43): 8068-8070.doi: 10.3969/j.issn.1673-8225.2011.43.022

• 骨与关节损伤基础实验 basic experiments of bone and joint injury • 上一篇    下一篇

种植早期大鼠种植体周围骨组织CXCR4蛋白的表达及意义

贾婷婷，侯玉东   

  1. 滨州医学院口腔学院，山东省滨州市  256603
  • 收稿日期:2011-05-09 修回日期:2011-07-11 出版日期:2011-10-22 发布日期:2011-10-22
  • 通讯作者: 侯玉东，副教授，硕士生导师，滨州医学院口腔学院，山东省滨州市256603 bycgk@126.com
  • 作者简介:贾婷婷★，女，1985年生，山东省青州市人，汉族，滨州医学院在读硕士，主要从事口腔修复研究。 jiatting6@sina.com

Expression and significance of CXCR4 in rat bone tissue around implants during early implantation

Jia Ting-ting, Hou Yu-dong   

  1. Dental Department of Binzhou Medical College, Binzhou  256603, Shandong Province, China
  • Received:2011-05-09 Revised:2011-07-11 Online:2011-10-22 Published:2011-10-22
  • Contact: Hou Yu-dong, Associate professor, Master’s supervisor, Dental Department of Binzhou Medical College, Binzhou 256603, Shandong Province, China bycgk@126.com
  • About author:Jia Ting-ting★, Studying for master’s degree, Dental Department of Binzhou Medical College, Binzhou 256603, Shandong Province, China jiatting6@sina.com

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297

被引次数

1

摘要：

背景：CXCR4与SDF-1构成SDF-1/CXCR4轴，在组织损伤及骨损伤修复过程中发挥重要作用。
目的：观察分析种植早期大鼠种植体周围骨组织CXCR4蛋白表达及其意义。
方法：Wistar大鼠20只，随机分为4组。将阳极氧化种植体植入除空白对照组外所有大鼠左侧胫骨。种植体植入6，12，24 h组分别于对应时间点处死大鼠，旋出种植体，截取种植体周围直径约2 cm骨组织，空白对照组截取胫骨近骺端直径约2 cm骨组织，制备蛋白样品。
结果与结论：从大鼠体内取出的各组阳极氧化种植体表面均可见大量的纤维成分附着。种植体植入6，12，24 h组CXCR4/β-actin灰度比值均明显高于空白对照组(P < 0.01)。种植体植24 h内种植体周围骨组织CXCR4表达量明显上调，提示CXCR4阳性细胞参与种植早期骨组织的修复。

关键词: 种植体, 免疫印迹法, 扫描电镜, CXCR4, 种植早期

Abstract:

BACKGROUND: The SDF-1/CXCR4 axis system is consisted of CXCR4 and stromal cell-derived factor -1 (SDF-1). The SDF-1/CXCR4 axis system plays an important role in regeneration and repair of tissue and bone injury.
OBJECTIVE: To investigate expression and significance of CXCR4 in rat bone tissue around implants during early implantation.
METHODS: Twenty Wistar rats of 4 months old were selected and randomly divided into implantation 6-, 12- and 24-hour groups and blank control group with 5 mice in each group. All rats except blank control group received anodically oxidized implants in the left tibia. Rats were killed 6, 12, 24 hours after implantation, the implants were unscrewed, and bone tissues around implants were prepared, 2 cm in diameter. For blank control group, 2 cm proximal epiphysis bone tissues were prepared. Then protein samples were then prepared.
RESULTS AND CONCLUSION: a large number of fibers could be seen on the anodized implant surface in each group. the grey values of CXCR4/β-actin were significantly higher in the implantation 6-, 12- and 24-hour groups than the blank control group (P > 0.01). The expression of CXCR4 during the first 24 hours of implantation was obviously higher than that of the blank control group, indicating that CXCR4 positive cells take part in the regeneration of bone tissue during early osseointegration.

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