中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (41): 7783-7786.doi: 10.3969/j.issn.1673-8225.2011.41.046

• 组织构建临床实践 clinical practice in tissue construction • 上一篇    下一篇

绝经后妇女骨代谢过程中血清基质金属蛋白酶3和骨桥蛋白的变化

张汉庆1，戴  燚1，李  浩2，郭张强3，殷  涛4，王  度5   

  1. 1武汉市中医医院骨科，湖北省武汉市430014
    2湖北省中医院，湖北省武汉市430060
    3武汉市普爱医院，湖北省武汉市430030
    4华中科技大学附属协和医院，湖北省武汉市430000
    5武汉市第三医院，湖北省武汉市 430000
  • 收稿日期:2011-06-27 修回日期:2011-08-19 出版日期:2011-10-08 发布日期:2011-10-08
  • 通讯作者: 戴燚，博士，武汉市中医医院骨伤科，湖北省武汉市430014 daiyifreeman@yahoo.com.cn
  • 作者简介:张汉庆☆，男，1969年生，湖北省武汉市人，汉族，2009年湖北中医药大学毕业，博士，副主任医师，主要从事中西医结合诊治骨质疏松症和骨性关节炎的研究。 zhqwh@yahoo.com.cn
  • 基金资助:

    湖北省卫生厅青年科技人才项目(QJX2010-48)，项目名称：绝经后骨质疏松症MMP-2/EGFR通路及益肾护骨方对其的影响；武汉市青年科技晨光计划项目(200950431215)，项目名称：绝经后骨质疏松症标志物基质金属蛋白酶的初步筛选；武汉市卫生局临床医学项目(WZ10C02)，项目名称：绝经后骨质疏松症血清标志物基质金属蛋白酶的初步筛选。

Serum levels of matrix metalloproteinase-3 and osteoprontin during bone metabolism in Chinese postmenopausal women

Zhang Han-qing1, Dai Yi1, Li Hao2, Guo Zhang-qiang3, Yin Tao4, Wang Du5   

  1. 1Department of Orthopedics, Wuhan Hospital of Traditional Chinese Medicine, Wuhan  430014, Hubei Province, China
    2Department of Orthopedics and Trauma, Hubei Hospital of Traditional Chinese Medicine, Wuhan  430060, Hubei Province, China
    3Pu’ai Hospital of Wuhan, Wuhan  430030, Hubei Province, China
    4Union Hospital Affiliated to Huazhong University of Science and Technology, Wuhan   430000, Hubei Province, China
    5Wuhan Third Hospital, Wuhan  430000, Hubei Province, China
  • Received:2011-06-27 Revised:2011-08-19 Online:2011-10-08 Published:2011-10-08
  • Contact: Dai Yi, Doctor, Department of Orthopedics, Wuhan Hospital of Traditional Chinese Medicine, Wuhan 430014, Hubei Province, China daiyifreeman@yahoo.com.cn
  • About author:Zhang Han-qing☆, Doctor, Associate chief physician, Department of Orthopedics, Wuhan Hospital of Traditional Chinese Medicine, Wuhan 430014, Hubei Province, China zhqwh@yahoo.com.cn
  • Supported by:

    a Project of Youth Talent in Science and Technology of Health Department of Hubei Province, No. QJX2010-48*; Youth Science and Technology Chenguang Development Program of Wuhan, No. 200950431215*; Clinical Medicine Program of Health Department of Wuhan, No. WZ10C02*

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295

被引次数

16

摘要：

背景：骨桥蛋白和基质金属蛋白酶3具有高度的亲和力，此二者的表达可能与骨代谢有关。
目的：观察绝经后妇女血清基质金属蛋白酶3和骨桥蛋白水平，并观察其与骨保护蛋白、骨保护蛋白配体及骨代谢指标的关系。
方法：将120名绝经后妇女分为骨密度正常组、低骨量组和骨质疏松组3 组，对其血清基质金属蛋白酶3、骨桥蛋白、骨保护蛋白、骨保护蛋白配体及骨碱性磷酸酶、骨钙素、Ⅰ型胶原交联C端肽和尿Ⅰ型胶原交联N端肽进行测定，计算骨桥蛋白/基质金属蛋白酶3比值。
结果与结论：骨质疏松组中血清骨桥蛋白和基质金属蛋白酶3的水平高于正常组(P < 0.05)。绝经后妇女血清基质金属蛋白酶3、骨桥蛋白和骨桥蛋白/基质金属蛋白酶3比值与血清骨保护蛋白配体、骨碱性磷酸酶和骨钙素水平呈明显负相关 (P < 0.05)，与骨保护蛋白、尿尿Ⅰ型胶原交联N端肽/肌酐比值呈明显正相关性(P < 0.05)。骨质疏松组中血清基质金属蛋白酶3、骨桥蛋白和骨桥蛋白/基质金属蛋白酶3比值与血清骨保护蛋白配体、骨碱性磷酸酶和骨钙素水平呈明显负相关 (P < 0.05)，与骨保护蛋白、尿尿Ⅰ型胶原交联N端肽/肌酐水平比值存在明显正相关性(P < 0.05)。提示绝经后妇女血清骨桥蛋白水平和骨桥蛋白/基质金属蛋白酶3比值升高与绝经后骨质疏松症伴随骨代谢转换过程增快有关。

关键词: 基质金属蛋白酶3, 骨桥蛋白, 骨保护蛋白, 绝经后骨质疏松, 骨组织工程

Abstract:

BACKGROUND: Osteopontin (OPN) has a great affinity for matrix metalloproteinase groups-3 (MMP-3). The expression of OPN and MMP-3 may play an important role in bone metabolism.
OBJECTIVE: To study the serum MMP-3 and OPN levels and the correlations of MMP-3 and OPN with bone metabolic markers and osteoprotegrin and its ligand in aged postmenopausal Chinese women.
METHODS: A total of 120 postmenopausal Chinese female volunteers were divided into three groups: normal bone mineral density, low bone mineral density, and osteoporosis. Serum levels of MMP-3, OPN, osteoprotegrin (OPG), osteoprotegrin ligand (OPGL), bone alkaline phosphate (BAP), osteocalcin, bone cross-linked N-telopeptides of typeⅠcollagen (NTx) and bone cross-linked C-telopeptides of typeⅠcollagen (CTx) were determined. The ratio of OPN to MMP-3 was also determined.
RESULTS AND CONCLUSION: Serum levels of OPN and MMP-3 were significantly higher in the osteoporosis group than in the normal bone mineral density group (P < 0.05). Serum levels of MMP-3 and OPN and OPN/MMP-3 were significantly negatively correlated with OPGL, BAP, and osteocalcin levels, but they were significantly positively correlated with OPG and the ratio of NTx to CTx (P < 0.05) in postmenopausal women. In the osteoporosis group, MMP-3, OPN, and the ratio of OPN to MMP-3 were significantly negatively correlated with OPGL, BAP and osteocalcin levels (P < 0.05), and they were significantly positively correlated with OPG and the ratio of NTx to CTx (P < 0.05). These findings suggest that the increases in serum level of OPN and ratio of OPN to MMP-3 appear possibly as a concomitant event in high bone turnover state, such as postmenopausal osteoporosis.

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