中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (33): 6135-6138.doi: 10.3969/j.issn.1673-8225.2011.33.014

• 组织构建实验造模 experimental modeling in tissue construction • 上一篇    下一篇

急性脊髓损伤模型大鼠与白细胞介素1受体拮抗剂的保护作用

黄颉刚1，宗少晖2，欧  超3，熊春翔2，赵玉玺2   

  1. 广西医科大学，1微生物学教研室，2第一附属医院脊柱骨病外科，3医学科学实验中心，广西壮族自治区南宁市 530021
  • 收稿日期:2011-04-20 修回日期:2011-07-05 出版日期:2011-08-13 发布日期:2011-08-13
  • 通讯作者: 宗少晖，博士，副主任医师，广西医科大学第一附属医院脊柱骨病外科，广西壮族自治区，南宁市 530021 xiaohui3008@126.com
  • 作者简介:黄颉刚★，男，1975年生，广西壮族自治区横县人，壮族，1998年广西医科大学毕业，硕士，讲师，主要从事分子免疫及微生物学研究。 katooo@163.com
  • 基金资助:

    课题由广西医科大学博士启动基金(308010)及广西教育厅科研基金(桂教200710LX063)资助，名称：白细胞介素1受体拮抗剂对急性脊髓损伤的组织学研究。

Effect of interleukin-1 receptor antagonist on neurofilament protein 200 and glial fibrillary acidic protein expression in a rat model of acute spinal cord injury

Huang Jie-gang1, Zong Shao-hui2, Ou Chao3, Xiong Chun-xiang2, Zhao Yu-xi2   

  1. 1Department of Microbiology, 2Department of Spine Surgery, First Affiliated Hospital of Guangxi Medical University, 3Central Laboratory of Medical Sciences, Guangxi Medical University Nanning   530021, Guangxi Zhuang Autonomous Region, China
  • Received:2011-04-20 Revised:2011-07-05 Online:2011-08-13 Published:2011-08-13
  • Contact: Zong Shao-hui, Doctor, Associate chief physician, Department of Spine Surgery, First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China xiaohui3008@126. com
  • About author:Huang Jie-gang★, Master, Lecturer, Department of Microbiology of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China katooo@163.com
  • Supported by:

    Doctoral Fund of Guangxi Medical University, No.308010*; Scienfific Research Fund of Education Department of Guangxi Zhuang Autonomous Region, No.guijiao200710LX063*

PDF

339

被引次数

2

摘要：

背景：白细胞介素1受体拮抗剂对大鼠急性脊髓损伤后脊髓功能修复具有保护作用，但具体机制不明。
目的：观察白细胞介素1受体拮抗剂对大鼠急性脊髓损伤组织神经丝蛋白质200和胶质纤维酸性蛋白的影响。
方法：SD大鼠随机分成假手术组，生理盐水对照组和白细胞介素1受体拮抗剂治疗组。采用改良Allen氏打击法建立急性脊髓损伤大鼠模型。分别在建模后1，48和72 h获取损伤段8 mm脊髓标本。
结果和结论：免疫组织化学染色检测，白细胞介素1受体拮抗剂治疗组神经丝蛋白质200和胶质纤维酸性蛋白的表达较假手术组和生理盐水组高，差异有显著性意义(P < 0.05)。提示白细胞介素1受体拮抗剂可使急性脊髓损伤大鼠模型损伤段脊髓神经丝蛋白质200和胶质纤维酸性蛋白表达增加，对急性脊髓损伤发挥保护作用。

关键词: 急性脊髓损伤, 白细胞介素1受体拮抗剂, 神经丝蛋白质200, 胶质纤维酸性蛋白, 炎症反应

Abstract:

BACKGROUND: Interleukin-1 receptor shows protective effects on spinal cord function recovery in rats with acute spinal cord injury, but the precise mechanism remains poorly understood.
OBJECTIVE: To investigate the effect of interleukin-1 receptor antagonist (IL-1Ra) on neurofilament protein 200 (NF 200) and glial fibrillary acidic protein (GFAP) expression in rat acute spinal cord injury tissue.
METHODS: A total of 90 Sprague-Dawley rats were randomly divided into three groups: sham-operated, normal saline and IL-1Ra treatment group. Acute spinal cord injury models were established by improved Allen’s method. At 1, 48 and 72 hours after model establishment, an 8-mm spinal cord segment was dissected longitudinally from the lesion site in each group. The NF200 and GFAP expression was detected by immunohistochemical staining.
RESULTS AND CONCLUSION: NF200 and GFAP expression in the IL-1Ra trentment group was significantly higher than that in the sham-operated and normal saline group (P < 0.05). These results showed that IL-1Ra can increase NF200 and GFAP expression in the injured spinal cord segment and exhibit protective effect on acute spinal cord injury.

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