中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (32): 6012-6015.doi: 10.3969/j.issn.1673-8225.2011.32.028

• 干细胞移植 stem cell transplantation • 上一篇    下一篇

嗅鞘细胞移植脊髓损伤大鼠Sema3A及其受体NP-1基因的表达

朱振东1，张  林2，周  雪3   

  1. 新乡医学院，1人体解剖学教研室，2法医物证学教研室，河南省新乡市   453003；3四川大学华西基础与法医学院组织胚胎和神经生物学教研室，四川省成都市610041
  • 收稿日期:2011-01-22 修回日期:2011-03-14 出版日期:2011-08-06 发布日期:2011-08-06
  • 通讯作者: 周雪，女，博士，教授，四川大学华西基础医学与法医学院组织胚胎学与神经生物学教研室，四川省成都市 610041 zhouxuel@mail.sc.cninfo.net
  • 作者简介:朱振东★，男，1982年生，山东省淄博市人，汉族，2008年四川大学华西医学中心毕业，硕士，助教，主要从事中枢神经损伤与修复的研究。 Stay0413@163.com

Effect of olfactory ensheathing cell transplantation on gene expression of Sema3A and its receptor, NP-1, in rats following spinal cord injury

Zhu Zhen-dong1, Zhang Lin2, Zhou Xue3   

  1. 1Department of Human Anatomy, 2Department of Forensic Biology, Xinxiang Medical College, Xinxiang  453003, Henan Province, China; 3Department of Histology, Embryology and Neurobiology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu  6l0041, Sichuan Province, China
  • Received:2011-01-22 Revised:2011-03-14 Online:2011-08-06 Published:2011-08-06
  • Contact: Zhou Xue, Doctor, Professor, Department of Histology, Embryology and Neurobiology, West China School of Preclinica1 and Forensic Medicine, Sichuan University, Chengdu 6l0041, Sichuan Province, China zhouxuel@mail.sc.cninfo.net
  • About author:Zhu Zhen-dong★, Master, Assistant teacher, Department of Human Anatomy, Xinxiang Medical College, Xinxiang 453003, Henan Province, China Stay0413@163.com

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摘要：

背景：嗅鞘细胞移植促进脊髓损伤修复机制中对神经生长抑制导向因子的研究较少。
目的：观察嗅鞘细胞移植对脊髓半横断损伤后神经生长抑制导向因子Sema3A及其受体NP-1在RNA水平表达的变化。
方法：17只SD大鼠随机分为嗅鞘细胞移植组、DMEM/F12 培养液移植组和正常对照组。制作T11~T12水平大鼠脊髓左侧半横断损伤模型，立刻分别注射嗅鞘细胞悬液或等量的DMEM/F12 培养液。
结果与结论：6周后取横断水平前后两个脊髓节段，用RT-PCR的方法对Sema3A、NP-1的mRNA进行半定量分析。①嗅鞘细胞在大鼠脊髓内仍有大量存活。②Sema3A及NP-1 mRNA的量，DMEM/F12 培养液移植组明显多于正常对照组(P < 0.05)，嗅鞘细胞移植组与培养液移植组比较明显下调，且与正常对照组比较差异无显著性意义(P > 0.05)。③提示嗅鞘细胞移植有效减少了神经生长抑制导向因子Sema3A及其受体NP-1 mRNA的表达。

关键词: 嗅鞘细胞, 脊髓, 半横断损伤, Sema3A, NP-1

Abstract:

BACKGROUND: Studies have proved that olfactory ensheathing cells (OECs) can stimulate the repair of spinal cord injury, but the research for mechanism of nerve growth inhibition guidance factor is rarely reported.
OBJECTIVE: To investigate the effects of OECs transplantation on the change of mRNA of Sema3A and NP-1.
METHODS: A total of 17 female adult healthy Sprague-Dawley rats were randomly divided into 3 groups: OECs transplanted group, DMEM/F12 transplanted group and normal control group. Transplanted groups were anaesthetized and then were acutely transplanted after the left spinal cord hemisection on the level of T11-12 with suspension of OECs or DMEM/F12 culture fluid.
RESULTS AND CONCLUSION: Two segments of the spinal cord adjacent to the hemisected segments were dissected 6 weeks later, and reverse transcription polymerase chain reaction (RT-PCR) was used for semi-quantitative analysis of the mRNA of Sema3A and NP-1. There were a large number of OECs alive 6 weeks later. The mRNA quantity of Sema3A and NP-1 in the DMEM/F12 transplanted group was more than that in the normal control group (P < 0.05). But the mRNA quantity of Sema3A and NP-1 in the OECs transplanted group was markedly down-regulated compared with the DMEM/F12 transplanted group, and there was no significant difference between OECs transplanted group and normal control group (P > 0.05). These indicated that the mRNA expression of Sema3A and NP-1 was effectively decreased by OECs transplantation.

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