中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (32): 5945-5950.doi: 10.3969/j.issn.1673-8225.2011.32.013

• 脂肪干细胞 adipose-derived stem cells • 上一篇    下一篇

残粒脂蛋白激活过氧化物酶体增殖物激活受体γ诱导人脂肪间充质干细胞的成脂分化

马美芳1，文  铁2，刘  玲1   

  1. 中南大学湘雅二医院，1心内科，2急诊外科，湖南省长沙市410011
  • 收稿日期:2011-05-20 修回日期:2011-06-12 出版日期:2011-08-06 发布日期:2011-08-06
  • 通讯作者: 刘玲，副教授，硕士生导师，中南大学湘雅二医院心血管内科，湖南省长沙市 410011 feliuling@medmail.com.cn
  • 作者简介:马美芳★，女，1981年生，河北省邯郸市人，汉族，2010年中南大学毕业，硕士，医师，主要从事残粒脂蛋白致动脉粥样硬化机制方面的研究。 wish0818@163.com
  • 基金资助:

    中华医学会动脉粥样硬化研究专项资金课题项目(09010570212)；湖南省杰出青年基金项目(10JJ1008)；中南大学湘雅二医院代谢综合征研究中心资助课题项目(DY-2008-01-01)。

Remnant-like particles activate peroxisome proliferator-activated receptor gamma and induce adipogenic differentiation of human adipose mesenchymal stem cells

Ma Mei-fang1, Wen Tie2, Liu Ling1   

  1. 1Department of Cardiology, 2Department of Surgical Emergency, Second Xiangya Hospital, Central South University, Changsha  410011, Hunan Province, China
  • Received:2011-05-20 Revised:2011-06-12 Online:2011-08-06 Published:2011-08-06
  • Contact: Liu Ling, Associate professor, Master’s supervisor, Department of Cardiology, Second Xiangya Hospital, Central South University, Changsha 410011, Hunan Province, China feliuling@medmail.com.cn
  • About author:Ma Mei-fang★, Master, Physician, Department of Cardiology, Second Xiangya Hospital, Central South University, Changsha 410011, Hunan Province, China wish0818@163.com
  • Supported by:

    Special Project Funds for Atherosclerosis Research of Chinese Medical Association, No. 09010570212*; Outstanding Youth Foundation of Hunan Province, No. 10JJ1008*; the Project Fund from Research Center of Metabolic Syndrome, Second Xiangya Hospital, Central South University, No. DY-2008-01-01*

PDF

489

摘要：

背景：残粒脂蛋白可诱导大鼠脂肪间充质干细胞分化为成熟的脂肪细胞。
目的：观察残粒脂蛋白对人脂肪间充质干细胞成脂分化和氧化物酶体增殖物激活受体γ、脂联素基因表达的影响。
方法：采用成脂诱导剂刺激人脂肪间充质干细胞，油红O染色鉴定脂肪细胞。分离高三酰甘油血症患者高脂餐后4 h抗凝血浆中的残粒脂蛋白，分别将0，50，100，150，200 mg/L残粒脂蛋白与10 mg/L胰岛素联合刺激脂肪间充质干细胞。
结果与结论：油红O染色发现0，50 mg/L 残粒脂蛋白组有微量脂滴形成。100~200 mg/L残粒脂蛋白组红色脂滴逐渐增加，以200mg/L组红色脂滴最多。在50~200 mg/L质量浓度范围内，细胞氧化物酶体增殖物激活受体γ mRNA与脂联素mRNA表达随残粒脂蛋白浓度增加而逐渐增强(P < 0.05)。200 mg/L残粒脂蛋白组脂联素 mRNA表达量显著低于150 mg/L组(P < 0.05)，但与100 mg/L 残粒脂蛋白组差异未达到显著性意义。表明残粒脂蛋白通过激活氧化物酶体增殖物激活受体γ诱导人脂肪间充质干细胞的成脂分化，并上调脂联素mRNA的表达。

关键词: 残粒脂蛋白, 氧化物酶体增殖物激活受体&gamma, (PPAR-&gamma, ), 脂肪间充质干细胞, 成脂分化, 脂联素

Abstract:

BACKGROUND: Remnant-like particles (RLPs) can induce rat adipose-derived mesenchymal stem cells (ADSCs) to differentiate into mature adipocytes.
OBJECTIVE: To explore the effects of RLPs on the adipogenic differentiation of human ADSCs and on mRNA expressions of peroxisome proliferator-activated receptor γ (PPAR-γ) and adiponectin (APN).
METHODS: After stimulation by standard cocktail inducer, lipid droplets within adipocytes were identified by oil red O staining. Human RLPs were isolated by density gradient ultracentrifugation and immuno-affinity chromatography from postprandial plasma of hypertriglyceridemic patients at 4 hours after a high-fat meal. ADSCs were stimulated by 10 mg/L insulin and different concentrations of RLPs (0, 50, 100, 150, 200 mg/L). 
RESULTS AND CONCLUSION: After stimulation at 12 days, a small amount of lipid droplets was found in the control group or  50 mg/L RLPs stimulated ADSCs by oil red O staining. Lipid droplets increased gradually with the increase of RLPs concentrations from 100 to 200 mg/L and reached the peak in 200 mg/L RLPs stimulated ADSCs. The mRNA expressions of PPAR-γ and APN increased with the increase of RLPs concentrations from 50-200 mg/L (P < 0.05). APN mRNA expression in 200 mg/L RLPs group was significantly weaker than that in 150 mg/L RLPs group (P < 0.05), while it showed no significant difference when compared with that in 100 mg/L RLPs group. It indicates that RLPs induce adipogenic differentiation of human ADSCs through activating PPARγ, and up-regulate APN expression.

中图分类号: