中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (25): 4637-4643.doi: 10.3969/j.issn.1673-8225.2012.25.016

• 材料生物相容性 material biocompatibility • 上一篇    下一篇

胶原/透明质酸膜与明胶海绵支架材料力学及组织相容性的比较

肖荣冬1，翁国星2   

  1. 1福建医科大学省立临床学院心外科，福建省福州市 350001；
    2福建省立医院心血管外科，福建省福州市 350001
  • 收稿日期:2011-11-05 修回日期:2011-11-05 出版日期:2012-06-17 发布日期:2013-11-04
  • 作者简介:肖荣冬☆，男，1973年生，福建省武平县人，汉族，2006年福建医科大学毕业，博士，副主任医师，主要从事组织工程血管研究。 xrddoctor@ yahoo.com.cn
  • 基金资助:

    福建省自然科学基金资助项目(2010J01131)

Comparison of material mechanics and histocompatibility between collagen/hyaluronic acid membrane and gelatin sponge scaffold

Xiao Rong-dong1, Weng Guo-xing2   

  1. 1Department of Cardiac Surgery, Provincial Clinical College of Fujian Medical University, Fuzhou 350001, Fujian Province, China;
    2Department of Cardiovascular Surgery, Fujian Provincial Hospital, Fuzhou 350001, Fujian Province, China
  • Received:2011-11-05 Revised:2011-11-05 Online:2012-06-17 Published:2013-11-04
  • About author:Xiao Rong-dong☆, Doctor, Associate chief physician, Department of Cardiac Surgery, Provincial Clinical College of Fujian Medical University, Fuzhou 350001, Fujian Province, China xrddoctor@ yahoo.com.cn

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731

被引次数

14

摘要：

背景：课题组前期实验证明胶原/透明质酸膜具有良好的力学性能和组织相容性。
目的：观察复合材料胶原/透明质酸膜及明胶海绵的生物学性能。
方法：应用材料复合交联的实验方法构建胶原/透明质酸膜并测定胶原/透明质酸膜、明胶海绵支架的力学性能。将支架材料种植于兔皮下，按照2，4，6，8，12周不同时间点评价材料在体内的降解情况和组织相容性。
结果与结论：①成功制备了胶原/透明质酸膜。②胶原/透明质酸膜具有较好的韧性和抗张强度，明胶海绵的力学性能不够理想。③两种材料在体内的降解均符合生物材料的组织反应过程，胶原/透明质酸膜在体内12周可完全降解，明胶海绵约6周完全降解。④胶原/透明质酸膜与平滑肌细胞的黏附率高，细胞的增殖和代谢状况较好，而明胶海绵的细胞黏黏附和增殖率相对较低。说明胶原/透明质酸膜具有较好的生物学性能。

关键词: 胶原/透明质酸膜, 明胶海绵, 生物学性能, 组织相容性, 降解, 增殖, 生物材料

Abstract:

BACKGROUND: Our previous studies have confirmed that collagen/hyaluronic acid membrane has good mechanical properties and histocompatibility.
OBJECTIVE: To explore the biological properties of collagen/hyaluronic acid membrane and gelatin sponge scaffold.
METHODS: The collagen/hyaluronic acid membrane was constructed by compound cross linking method. The mechanical property of the collagen/hyaluronic acid membrane and gelatin sponge scaffold was detected. The scaffold materials were implanted subcutaneously in rabbits; the in vivo degradation and mechanical properties were evaluated at 2, 4, 6, 8 and 12 weeks after implantation.
RESULTS AND CONCLUSION: Collagen/hyaluronic acid membrane was constructed successfully. Collagen/hyaluronic acid membrane had a better toughness and tensile strength while the mechanical property of gelatin sponge was not ideal. The in vivo degradation of the two materials was compatible with the tissue reactions of biomaterials. Complete degradation of the collagen/hyaluronic acid membrane could be done within 12 weeks, while the gelatin sponge within 6 weeks. The adhesion rate between the collagen/hyaluronic acid membrane and smooth muscle cells was high; the cell proliferation and the metabolic status were good. The adhesion rate and proliferation rate of the gelatin sponge was relatively low. These findings indicate that gelatin sponge has better biological properties.

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