中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (47): 8817-8820.doi: 10.3969/j.issn.2095-4344.2012.47.017

• 生物材料基础实验 basic experiments of biomaterials • 上一篇    下一篇

三种不同方法制备去细胞神经支架的比较

李晓峰，赵劲民，秦义武，罗世兴，程建文   

  1. 广西医科大学第一附属医院创伤骨科手外科，广西壮族自治区南宁市 530021
  • 收稿日期:2012-01-17 修回日期:2012-03-11 出版日期:2012-11-18 发布日期:2013-03-15
  • 通讯作者: 赵劲民，博士，教授，广西医科大学第一附属医院创伤骨科手外科，广西壮族自治区南宁市 530021 zhaojinmin@ 126.com
  • 作者简介:李晓峰☆，男，1974年生，吉林省德惠县人，汉族，2011年广西医科大学毕业，博士，主治医师，主要从事创伤外科手外科、组织工程研究。 xiaofengli74@ 163.com

Comparison of acellular nerve grafts prepared with three different methods

Li Xiao-feng, Zhao Jin-min, Qin Yi-wu, Luo Shi-xing, Cheng Jian-wen   

  1. Department of Traumatic Orthopedics and Hand Surgery, First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China
  • Received:2012-01-17 Revised:2012-03-11 Online:2012-11-18 Published:2013-03-15
  • Contact: Zhao Jin-min, Doctor, Professor, Department of Traumatic Orthopedics and Hand Surgery, First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China zhaojinmin@126.com
  • About author:Li Xiao-feng☆, Doctor, Attending physician, Department of Traumatic Orthopedics and Hand Surgery, First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China xiaofengli74@ 163.com

PDF

442

被引次数

7

摘要：

背景：去细胞异体神经移植物具有完全仿真的三维结构，为细胞附着提供了较理想的三维空间，是目前所有人工合成材料所无法比拟的。
目的：对比分析液氮冷冻法、化学法及改良法3种方法对大鼠坐骨神经去细胞化的优劣。
方法：切取SD大鼠10 mm坐骨神经，分别以液氮冻融法、TritonX-100处理化学去细胞法、改良法处理的化学去细胞法分别制作去细胞神经支架，行苏木精-伊红染色，并在扫描电镜下观察支架超微结构。
结果与结论：液氮冻融法去细胞支架内有较多许旺细胞残留，有较多轴突及髓鞘，基膜完整，基膜管欠通畅；TritonX-100处理化学法去细胞神经支架内无明显许旺细胞，管内未见明显轴突及髓鞘残留，基膜完整，基膜管通畅；改良法处理的化学去细胞神经支架内许旺细胞及轴突、髓鞘成分几乎完全消失，去细胞效果更好，基膜完整，基膜管更通畅。表明TritonX-100处理化学去细胞法、改良法处理的化学去细胞法脱细胞效果较好，其中以改良法处理的化学去细胞法最好。

关键词: 神经支架, 去细胞, 同种异体, 人工神经, 组织工程

Abstract:

BACKGROUND: Acellular allogeneic nerve graft has a three-dimensional structure which is emulational completely, and provides an ideal three-dimensional space for cell adhesion. These are unmatchable for all synthetics at present.
OBJECTIVE: To contrast and analyze the pro and con of the liquid nitrogen cryopreservation, chemical method and improved chemical method for preparation of acellular sciatic nerve.
METHODS: 10-mm sciatic nerve segments of SD rats were selected, and treated with liquid nitrogen cryopreservation, TritonX-100 chemical, and improved chemical methods. The acellular nerve grafts were prepared for hematoxylin-eosin staining. The ultrastructure of the grafts was observed under the scanning electron microscopy.
RESULTS AND CONCLUSION: Acellular nerve grafts prepared by liquid nitrogen cryopreservation were found more residual schwann cells, more axons and myelin. Grafts prepared by chemical method with TritonX-100 had no various schwann cells, as well as no significant residual axons and myelin were seen; the inophragma was intact and inophragma tube was clear. In the acellular nerve grafts prepared by improved chemical method, Schwann cells, axons and myelin almost disappeared; the acellular effect was better, and the inophragma was intact, besides, nophragma tube was clearer. These findings suggest that the acellular effects of chemical method and improved chemical method are better, and the improved chemical method is the best.

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