中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (10): 1794-1798.doi: 10.3969/j.issn.1673-8225.2011.10.018

• 干细胞移植 stem cell transplantation • 上一篇    下一篇

鼻腔内长期输送脐血单个核细胞对大鼠脑梗死体积及行为学的影响

曾宪智1，沈慧娟2，王  洋3，朱群娥4，郭连军4，叶先才4   

  1. 嘉兴学院医学院，1解剖学教研室，  3临床医学系，4医学实验中心，浙江省嘉兴市314001；2嘉兴学院机电学院机电工程实验中心，浙江省嘉兴市314001
  • 收稿日期:2010-12-24 修回日期:2011-02-07 出版日期:2011-03-05 发布日期:2011-03-05
  • 作者简介:曾宪智☆，男，1968年生，福建省莆田市人，汉族，2006年首都医科大学毕业，博士，讲师，主要从事中枢神经解剖学，干细胞，神经损伤机制和防治基础研究。 frankzengxzh@yahoo.cn
  • 基金资助:

    浙江省嘉兴市科技计划项目(2010AY1097)；浙江省大学生科技创新项目(2010R417021)。

Effects of long term intranasal delivering umbilical cord blood mononuclear cells on cerebral infarct volume and behaviors in rats

Zeng Xian-zhi1, Shen Hui-juan2, Wang Yang3, Zhu Qun-e4, Guo Lian-jun4, Ye Xian-cai4   

  1. 1Department of Anatomy, College of Medicine, Jiaxing University, Jiaxing  314001, Zhejiang Province, China; 2Laboratory Center of  Machinery and Electronics Engineering, College of Machinery and Electronics Engineering, Jiaxing University, Jiaxing  314001, Zhejiang Province, China; 3Clinical Department, College of Medicine, Jiaxing University, Jiaxing  314001, Zhejiang Province, China; 4Medical Laboratory Center, College of Medicine, Jiaxing University, Jiaxing  314001, Zhejiang Province, China
  • Received:2010-12-24 Revised:2011-02-07 Online:2011-03-05 Published:2011-03-05
  • About author:Zeng Xian-zhi☆, Doctor, Lecturer, Department of Anatomy, College of Medicine, Jiaxing University, Jiaxing 314001, Zhejiang Province, China frankzengxzh@yahoo.cn
  • Supported by:

    the Science and Technology Program of Jiaxing City of Zhejiang Province, No. 2010AY1097*; the Science and Technology Innovation Program of Undergraduate of Zhejiang Province, No. 2010R417021*

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被引次数

1

摘要：

背景：通过鼻腔输送细胞和具有生物活性的因子到大脑内是一种新的非侵入性输送方法，克服了其他给药方法受到血脑屏障限制和产生外周不良反应的缺点。
目的：观察鼻腔内长期输送人脐血单个核细胞大鼠脑梗死梗死体积及行为学的变化。
方法：直接切断Wistar大鼠一侧大脑中动脉制作脑梗死模型，将神经功能评分相当的模型大鼠，抽签随机分成对照组、单个核细胞组，随即单个核细胞组从鼻腔滴入培养的第3代人脐血单个核细胞，对照组滴入磷酸盐缓冲液，1次/d，共6周，每周进行1次行为学评价；最后1次行为学评价后行脑梗死体积量化检测。
结果与结论：与对照组比较，治疗后3周单个核细胞组脑梗死大鼠改良的神经病学严重程度评分开始改善，之后进一步改善到5周(P < 0.05，P < 0.01)；治疗后1~5周单个核细胞组Morris水迷宫实验平均潜伏期明显缩短(P < 0.05，P < 0.01)，并且随着时间的延长，效果越明显。与行为学改善相一致，单个核细胞组脑梗死体积(9.15±4.36)%较对照组(30.56±4.65)%显著缩小(P < 0.01)。说明经鼻腔早期长期输注单个核细胞治疗大鼠脑梗死能改善其行为能力和缩小脑梗死体积，是治疗脑梗死的一种简便易行治疗方法。

关键词: 脑梗死, 脐血, 单个核细胞, 大鼠, 干细胞

Abstract:

BACKGROUND: It was a new non-invasive method for intranasal delivering cell or cytokine into the brain, which could overcome the peripheral side-effect of other delivering methods.
OBJECTIVE: To explore the effect of long term intranasal delivering human umbilical cord blood mononuclear cells (UCBMCs) on changes in behaviors and infarct volume of brain infarct in rats.
METHODS: Middle cerebral artery of Wistar rat was directly cut to establish models of cerebral infarction. Rat models with equal neurological score were randomly assigned to control and mononuclear cell groups. In the mononuclear cell group, UCBMCs of the third generation were intranasally injected. In the control group, phosphate buffer saline was infused, once a day, totally 6 weeks. Behavior was assessed once a week. Brain infarct volumes were quantified following the last behavior assessment.
RESULTS AND CONCLUSION: Compared with control group, modified neurological severity score was improved in the mononuclear cell group at 3 weeks after treatment, and lasted at 5 weeks (P < 0.05, P < 0.01). In the mononuclear cell group, the mean latency in Morris Water Maze Test shortened significantly at 1-5 weeks following treatment (P < 0.05, P < 0.01), and the effect was obvious over time. Identical to behavior improvement, the cerebral infarct volume in the mononuclear cell group (9.15±4.36)% was smaller compared with (30.56±4.65)% (P < 0.01). Early and long term of intranasal delivering UCBMCs to treat brain infarct model rats could improve their behavior score and shrink their infarct volume, which could be considered as a simple and feasible method to treat brain infarct.

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