中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (6): 1075-1078.doi: 10.3969/j.issn.1673-8225.2011.06.027

• 干细胞基础实验 basic experiments of stem cells • 上一篇    下一篇

急性淋巴细胞白血病患者骨髓单个核细胞TAp63基因的表达

潘阳琼1，刘延方1，2，孙  慧1，王桂叶1   

  1. 1郑州大学第一附属医院血液科，河南省郑州市450052
    2河南省高等学校临床医学重点学科开放实验室，河南省郑州市  450052
  • 收稿日期:2010-08-31 修回日期:2010-11-01 出版日期:2011-02-05 发布日期:2011-02-05
  • 通讯作者: 刘延方，教授，主任医师，郑州大学第一附属医院血液内科，河南省郑州市450052
  • 作者简介:潘阳琼★，女，1982年生，河南省信阳市人，汉族，郑州大学第一附属医院在读硕士，主要从事造血系统疾病基础与临床研究。 panyangqiong@126.com

TAp63 gene expression in bone marrow mononuclear cells derived from acute lymphoblastic leukemia patients

Pan Yang-qiong1, Liu Yan-fang1, 2, Sun Hui1, Wang Gui-ye1   

  1. 1Department of Hematology, First Affiliated Hospital, Zhengzhou University, Zhengzhou  450052, Henan Province, China
    2Key Laboratory of Clinical Research for Henan University, Zhengzhou  450052, Henan Province, China
  • Received:2010-08-31 Revised:2010-11-01 Online:2011-02-05 Published:2011-02-05
  • Contact: Liu Yan-fang, Professor, Chief physician, Department of Hematology, First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, Henan Province, China; Key Laboratory of Clinical Research for Henan University, Zhengzhou 450052, Henan Province, China
  • About author:Pan Yang-qiong★, Studying for master’s degree, Department of Hematology, First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, Henan Province, China panyangqiong@126.com

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317

被引次数

3

摘要：

背景：据作者查新检索，国内外有关急性白血病患者骨髓单个核细胞TAp63基因表达的报道罕见。
目的：观察急性淋巴细胞白血病患者骨髓单个核细胞TAp63基因的表达及其意义。
方法：50例急性淋巴细胞白血病患者，其中32例急性B淋巴细胞白血病，18例急性T淋巴细胞白血病。同期选择27例非恶性血液病患者作为对照。取肝素抗凝的骨髓液2~4 mL，用Ficoll液分离骨髓单个核细胞，半定量反转录聚合酶链反应法检测TAp63的表达。
结果与结论：50例急性淋巴细胞白血病患者有49例表达TAp63，急性淋巴细胞白血病组明显高于非恶性血液病组(P < 0.05)，急性B淋巴细胞白血病表达水平显著高于急性T淋巴细胞白血病(P < 0.05)。动态观察了5例初治急性淋巴细胞白血病化疗后不同阶段TAp63的表达变化，发现初治时TAp63表达，缓解后低表达或不表达，复发后又表达。结果表明TAp63在急性淋巴细胞白血病患者骨髓单个核细胞的表达明显高于非恶性血液病患者，尤其在急性B淋巴细胞白血病患者中高表达。

关键词: 急性淋巴细胞白血病, 骨髓, TAp63, 表达, 半定量反转录聚合酶链反应

Abstract:

BACKGROUND: The expression of TAp63 gene in bone marrow mononuclear cells derived from acute lymphoblastic leukemia (ALL) patients has been rarely reported.
OBJECTIVE: To investigate the expression and significance of TAp63 gene in bone marrow mononuclear cells of adult ALL.
METHODS: A total of 50 cases of ALL, including 32 cases of B-ALL and 18 cases of T-ALL, were enrolled. A total of 27 cases of adults without malignance disease served as control group. A total of 2-4 mL heparin anticoagulant bone marrow was collected. Bone marrow mononuclear cells were isolated using Ficoll liquid. TAp63 expression was examined by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR).
RESULTS AND CONCLUSION: A total of 49 cases of 50 ALL cases expressed TAp63. The expression of TAp63 in ALL group was significantly higher than in control group (P < 0.05). The expression of TAp63 in B-ALL group was significantly higher than that of T-ALL group (P < 0.05). By sequential observation of TAp63 expression in different stages of ALL in 5 cases, TAp63 mRNA expression in patients who were in de novo and relapsed stages was significantly higher than that in complete remission stage. These results demonstrated that the expression of TAp63 in ALL patients was significantly higher than that of control group, especially in B-ALL.

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