中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (5): 774-777.doi: 10.3969/j.issn.1673-8225.2011.05.004

• 肝移植 liver transplantation • 上一篇    下一篇

大鼠肝移植急性排斥反应模型的建立

樊  斌，李德卫，冯  渊，杨晓波   

  1. 重庆医科大学附属第一医院肝胆外科，重庆市 400016
  • 收稿日期:2010-07-05 修回日期:2010-08-09 出版日期:2011-01-29 发布日期:2011-01-29
  • 通讯作者: 李德卫，副教授, 重庆医科大学附属第一医院肝胆外科，重庆市 400016 lidewei406@ sina.com
  • 作者简介:樊斌★，男，1984年生，湖北省安陆市人，汉族，重庆医科大学在读硕士，主要从事肝胆外科研究。 fb824@126.com

Establishment of an acute rejection model of rat orthotopic liver transplantation

Fan Bin, Li De-wei, Feng Yuan, Yang Xiao-bo   

  1. Department of Hepatobiliary Surgery, the First Affiliated Hospital, Chongqing Medical University, Chongqing  400016, China
  • Received:2010-07-05 Revised:2010-08-09 Online:2011-01-29 Published:2011-01-29
  • Contact: Li De-wei, Associate professor, Department of Hepatobiliary Surgery, the First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China lidewei406@sina. com
  • About author:Fan Bin★, Studying for master’s degree, Department of Hepatobiliary Surgery, the First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China fb824@126.com

PDF

887

被引次数

6

摘要：

背景：研究表明Wistar和SD大鼠普遍为封闭杂交系而非近交系，有一定的遗传稳定性，同时也有一定的基因多态性，因此Wistar和SD大鼠之间的肝移植模型可能不是研究大鼠急性排斥反应的理想模型。
目的：建立Lewis-BN大鼠肝脏移植急性排斥反应模型。
方法：采用改良的“Kamada”二袖套法，分别进行同基因Lewis-Lewis间肝移植及Lewis-BN间肝移植。移植后3，5，7 d观察受体肝脏组织病理变化，测定谷丙转氨酶和总胆红素水平变化及大鼠存活时间。
结果与结论：同基因移植组大鼠无急性排斥表现，平均存活时间超过100 d，肝功能损害较轻。异基因移植组大鼠存活 (12.75±1.25) d，移植后第7 天肝脏病理检查有明显的排斥反应，肝功能损害较重，移植后各时相点谷丙转氨酶和总胆红素水平均明显高于同基因移植组(P < 0.05)。提示Lewis-BN大鼠肝移植组合为稳定的大鼠肝移植急性排斥模型，但近交系大鼠对手术耐受性差，建模难度大，熟练的显微外科技术和细心轻柔的操作是模型成功的关键。

关键词: 排斥反应, 肝移植, 大鼠, 近交系, 器官移植

Abstract:

BACKGROUND: Some research indicates that the Wistar and Sprague Dawley rats belong to closed colony strains, rather than inbred strains, which not only has some hereditary stability but also genetic polymorphism. Thus, the Wistar to SD rat liver transplantation models may not be the ideal models to study the acute rejection of rat liver transplantation.
OBJECTIVE: To establish an acute rejection model of Lewis-BN rat orthotopic liver transplantation.
METHODS: The model was established by modified Kamada’s two-cuff technique. The rats were performed Lewis to Lewis or Lewis to BN rat liver transplantation. The pathological changes of transplanted livers were observed and levels of alaine aminotransferase (ALT) and total bilirubin (TBIL) were detected at 3, 5 and 7 days after transplantation. The survival time of rats was determined. 
RESULTS AND CONCLUSION: There was no rejection in isogene group from Lewis to Lewis rat, and the average survival time was over 100 days. Liver function was mildly impaired. Conversely, the average survival time was (12.75±1.25) days in the allogene group. The pathological examination showed a typical acute rejection at 7 days. Liver function was severely impaired. At the same time point after the operation, levels of ALT and TBIL in the allogene group were significantly higher than those of the isogene group (P < 0.05). The results indicate that, Lewis to BN rat combination is a stable acute rejection model of rat liver transplantation, but there may be technical difficulties due to its poor tolerance. The sophisticated microsurgical technique and the delicate surgical manipulation is the key to achieve success.

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